We take frozen section from formalin fixed tissue and placed them in
30% sucrose overnight before mounting on chucks.
But never tried the other way round frozen tissue then fixed formalin, i
heard that it helps in falling of the tissue. But no experience.
Amita
From: "Sherwood, Marg
Does anyone have a letter from there waste water municipality accepting any of
the chemicals used in the histo lab (alcohol or neutralized 10%NBF)? Does any
one treat there 10%NBF and dispose of it down the sink?
Have A Great Day!
Sherri Neely
_
Job Title: Histology Technician fulltime Porter campus - $3000 Sign On Bonus
Department:
40100 -- Laboratory
Job Type:
Full Time
Shift Info:
Mon-Fri/ 2 half day Saturday shifts/mon
Expertise:
Allied Health
Job Description:
Performs histo
We use the expiration date on the formalin as the use by date. By the time the
solution would be out of date, the tissues should be very well fixed, so the
formalin becomes just a holding solution.
Joe Saby
NAMSA
From: "amitapan...@torrentpharma.com"
To: hi
Peggy,
We fix ours prior to freezing and cryosectioning routinely. You can use 10% NBF
(commercial prep) or you can make your own using paraformaldehyde, 4% in PBS
buffer. After fixation, you will need to cryoprotect in sucrose/PBS. We fix,
then rinse, and then place in 15% sucrose/PBS until th
Formalin fixed FS before embedding and sectioning? That is quite confuse.
Your FS should be fixed before H&E staining (acetone or even 10% NBF will do).
If you refer to the specimen that was frozen to do the FS, before being
embedded and cut, has to be fixed to become a FFPE specimen.
René J.
Fr
Not before embedding in OCT, but we have 10%nbf as our first station in the
staining process. The slides stay in there for 1 minute, water rinse, then
proceed with your stain as usual.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utso
If you have a fumes hood, and a body emergency shower you should QC them along
with your drying oven microtomes and tissue processor. Any balance you may have
also should be QC'd.
René J.
From: Amber McKenzie
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, May 18, 2011 3:36 PM
Subject: [
Does anyone fix their frozen tissue first in formalin before embedding and
sectioning? I know one investigator in our lab did that previously, but was not
sure of the strength of formalin (I don't believe it was 10%). Another
investigator inquired about doing that.
I would appreciate hearing fro
I am updating my QC Manual and wanted to know if you QC your Processors
and Microtomes? These are the only 2 pieces of equipment that I
haven't been QC'ing.
Here's what I QC each month, am I covering everything?
1. Eye wash station
2. Fridge temp
3. 10%formalin
4.
Tim,
The thickest paraffin sections I have cut are 50 microns and they curled so
tightly right off the blade I can't imagine what you might get with a 100
micron section. Even if you do get this slab intact, I think the tissue would
show cracking artifact, not to mention the big chunks taken ou
Kathy
We have an Aperio Scanscope XT and scan for a fee
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado
Hello,
I am looking for a slide scanner like Aperio to scan my lung tissue slides at
high quality and inclusive of increasingly higher magnification objectives. I
have the lite version of Aperio to view slides I scanned in when training at MD
Anderson in Houston using their Aperio and would
For those who stain smears, if you stain with PAS/PAP, would/do you charge for
one stain or two. And if you have more that one smear, do you charge for each
smear?
Thank you
Candy
Candy Bales, HT
Chief Histologist
The University of Texas Dental Branch at Houston
Diagnostic Sciences-Oral Pathol
Last year I was lucky to be a part of a medical mission in Belize. While there
I assisted a dermatologist. This year we are looking for an additional derm dr.
(maybe one who feels comfortable reading out slides) and a plastic surgeon to
expand on the medical part. There are family practice and E
What CPT code is everyone using for stomach sleeve reduction surgery for
morbid obesity?
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
Hi Histonetters
I am testing the Leica Bond III immunostainer. How is everyone handling
processing the Hercept test from Dako on the Bond III? I cannot use either
the approved pretreatment or visualization system, not to mention the
approved buffer. I currently run my immunos on the Dako so it has
We treat certain antibodies (including E-Cad, and many other breast markers) by
putting the slides into formalin for 15 minutes after baking. Then, rinse in
tap and put on the machine. It helps keep tissue on the slides. Also, as an
aside, I heard directly from someone who works for Erie (who
We've always had problems with the tissue falling off on the E-Cad
(Ventana). Normally, we put the patient tissue on the same slide as the
control tissue - but for the E-Cad, we always put the patient tissue on
a slide all by itself. This doesn't completely solve the problem, but
it's better.
Lau
We also experienced tissue adhesion problems. We have done several
things to address the issue:
We wear gloves when cutting controls to decrease any grease from our
fingers, we also dip the slide into the water bath upside down as to
leave the portion of the slide for the patient section untouched
Sounds to me like you may want to check your slide lots to make sure the charge
is consistent. I have purchased slides were there was variability within the
back from slide to slide. Also, if you use CC1 and CC2 I would check to make
sure that the solutions were not inadvertently switched, since
Good Morning,
I'm posting this for a non-member. Does anyone know of a lab that does
nonspecific esterase staining? A client needs it done on mouse tissue, and we
don't perform that test here in our histo lab.
Jan Shivers
University of Minnesota
Veterinary Diagnostic Laboratory
shive...@umn.e
Hello Histonet,
We've been having a recurring problem with E-cadherin from Ventana on Benchmark
Ultra. It's spanned two different lots of antibody. Our tissues are suddenly
falling off the slides. Negative controls are ok, just seems to be after
antibody treatment. The protocol hasn't chan
Hi Dr Dubey
Thanks for your message regarding choice of filter papers. Here in the U.K.
Whatman No 1 is almost universally used for filtration of all reagents and
solutions used in microscopy or general chemistry preparation. Other grades
of filter paper are available for other applications, but N
You can use Histogel as well from Richard Allan (Fisher or VWR)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA
SREBOTNIK KIRBIS
Sent: Wednesday, May 18, 2011 4:51 AM
To: kst...@mcw.edu; histonet@lists
we made a cell block from cell line by following procedure:
- centrifuge cell suspension
- fix the sediment overnight in formalin (for tissue fixation) - optional, the
button will be anyway fixed by subsequent procedure
- centrifuge and decant the formalin
- add few drops of liquid agar (not too
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