Great opportunity for a Histotechnician in a brand new laboratory! We
are a 5 physician gastroenterology practice located in Las Vegas, NV
looking for a certified HT or HTL. Candidate must meet CLIA grossing
requirements. The candidate will be responsible for routine histology
duties. This is
Hi Wassan,
you may depart the FISH procedure in a tissue-dependant first part and a
probe-dependant second part.
The first part comprises the pretreatment of the tissue to retrieve and
permeabilises it. The amount of pretreatment is dependant on the
fixation-duration of the tissue.
The longer the f
Dear members
We just started doing FISH using HER2 FISH pharma Dx Dako kit, Code number
K5331
on malignant breast tissues.
I was wondering what is the success ratio or percentage for say a one run?? my
hospital is the first in our region to start FISH
and I am the first to run it. I have no
If you haven't made up your mind yet on this issue, here are a couple of
references that may - or may not - help:
Veterinary Diagnostics:
http://www.ncbi.nlm.nih.gov/sites/entrez?orig_db=PubMed&db=pubmed&cmd=Search&term=%22Journal%20of%20veterinary%20diagnostic%20investigation%20%3A%20offici
Allison and Dr. Richmond:
We are using Novec 7000 successfully in our freezing bath and have been
doing so for about 6 months with no major problems.
It does have some annoying traits ; however the safety benefits outweigh
those issues , in my opinion. The issues are: a little more time required
I accidentally put my renal slides in the Methanimine Silver (after
Periodic Acid) before putting them in the Thiosemicarbizine. Slides
take on a yellow hue which makes the H&E counterstain muddy. Is there a
way to correct this?
Thank you,
Sally Norton
Histotech
CONFIDENTIALITY
Allison Scott after a flash fire with the stuff asks about
alternatives to 2-methylbutane for freezing specimens.
There is indeed a safe alternative, though I haven't had a chance to
try it. I've posted about it on Histonet more than once - here's a
copy of an earlier post of mine:
3M(tm) Novec(t
I believe the only place you can buy p63 is through Biocare, www.biocare.net.
Lynne Bell, HT (ASCP)
Histology Team Leader
802-371-4923
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Dear Miss Margaret Blount:
Congratulations on your retirement!! May you enjoy good health and many years
doing the things you WANT to do!!
God Bless!
-Original Message-
From: histonet-request
To: histonet
Sent: Mon, May 23, 2011 1:00 pm
Subject: Histonet Digest, Vol 90, Issue 28
Biocare Medical
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo
I need to find a source for p63 antibody. Where does everyone purchase theirs
from?
Joanne Clark,HT
Pathology Consultants of New Mexico
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Hello to all in histoland. What type of solutions are being used if any
to freezze your chucks for frozen sections. Our sister hospital is
using methyl butane and there was a flash fire last week. We use the
same solution. Any suggestions will be greatly appreciated.
Allison Scott HT(ASCP)
His
Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that
had already been in formailin two weeks (was FFPE). Normally would do
on a frozen 1:40? We have background everywhwere on first
run...suggestions? Neg is also shining??? Crazy researcher's! Got to
love them...when will they
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Sunday, May 22, 2011 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 90, Issue 27
Also Sprach Zarathustra
René J.
From: Carrie Disbrow
To: histonet@lists.utsouthwestern.edu
Sent: Sunday, May 22, 2011 10:00 PM
Subject: [Histonet] Immuno controls
Hi all,
After consideration I disagree with the consensus and also agree with Curt's
pathologist. More supplies and labor wil
Good Moorning to everyone:
I am Carmen Garcia a PhD student from Valencia, we are know interesting
in make in vitro culture of mouse embryos to do toxicity tests and in
our lab we have not experience. someone can give me some advise? or
recommend me some bibliography?
how long can I have the
To everyone in histoland,
This is my last full week at work. From next Tuesday I will be retiring
from my job at Cambridge University. I have thoroughly enjoyed my chosen
profession as a research technician specialising in histology. I realise
that because I have always worked in research my ex
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