[Histonet] Las Vegas Position

2011-05-23 Thread Hale, Meredith
Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is

AW: [Histonet] FISH

2011-05-23 Thread Gudrun Lang
Hi Wassan, you may depart the FISH procedure in a tissue-dependant first part and a probe-dependant second part. The first part comprises the pretreatment of the tissue to retrieve and permeabilises it. The amount of pretreatment is dependant on the fixation-duration of the tissue. The longer the f

[Histonet] FISH

2011-05-23 Thread wassan alkadhumi
Dear members   We just started doing FISH using HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant breast tissues.  I was wondering what is the success ratio or percentage for say a one run?? my hospital is the first in our region to start FISH   and I am the first to run it. I have no

[Histonet] IHC Control Tissues

2011-05-23 Thread Goins, Tresa
If you haven't made up your mind yet on this issue, here are a couple of references that may - or may not - help: Veterinary Diagnostics: http://www.ncbi.nlm.nih.gov/sites/entrez?orig_db=PubMed&db=pubmed&cmd=Search&term=%22Journal%20of%20veterinary%20diagnostic%20investigation%20%3A%20offici

Re: [Histonet] Re: Freezing bath solutions

2011-05-23 Thread mwhite
Allison and Dr. Richmond: We are using Novec 7000 successfully in our freezing bath and have been doing so for about 6 months with no major problems. It does have some annoying traits ; however the safety benefits outweigh those issues , in my opinion. The issues are: a little more time required

[Histonet] Jones stain boo boo

2011-05-23 Thread Norton, Sally
I accidentally put my renal slides in the Methanimine Silver (after Periodic Acid) before putting them in the Thiosemicarbizine. Slides take on a yellow hue which makes the H&E counterstain muddy. Is there a way to correct this? Thank you, Sally Norton Histotech CONFIDENTIALITY

[Histonet] Re: Freezing bath solutions

2011-05-23 Thread Robert Richmond
Allison Scott after a flash fire with the stuff asks about alternatives to 2-methylbutane for freezing specimens. There is indeed a safe alternative, though I haven't had a chance to try it. I've posted about it on Histonet more than once - here's a copy of an earlier post of mine: 3M(tm) Novec(t

[Histonet] RE: p63

2011-05-23 Thread Bell, Lynne
I believe the only place you can buy p63 is through Biocare, www.biocare.net. Lynne Bell, HT (ASCP) Histology Team Leader 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histonet Digest, Vol 90, Issue 28

2011-05-23 Thread boneslides
Dear Miss Margaret Blount: Congratulations on your retirement!! May you enjoy good health and many years doing the things you WANT to do!! God Bless! -Original Message- From: histonet-request To: histonet Sent: Mon, May 23, 2011 1:00 pm Subject: Histonet Digest, Vol 90, Issue 28

[Histonet] RE: p63

2011-05-23 Thread McMahon, Loralee A
Biocare Medical Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo

[Histonet] p63

2011-05-23 Thread Joanne Clark
I need to find a source for p63 antibody. Where does everyone purchase theirs from? Joanne Clark,HT Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histon

[Histonet] Freezing bath solutions

2011-05-23 Thread Scott, Allison D
Hello to all in histoland. What type of solutions are being used if any to freezze your chucks for frozen sections. Our sister hospital is using methyl butane and there was a flash fire last week. We use the same solution. Any suggestions will be greatly appreciated. Allison Scott HT(ASCP) His

[Histonet] RE: Histonet Digest, Vol 90, Issue 27

2011-05-23 Thread Barone, Carol
Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that had already been in formailin two weeks (was FFPE). Normally would do on a frozen 1:40? We have background everywhwere on first run...suggestions? Neg is also shining??? Crazy researcher's! Got to love them...when will they

[Histonet] RE: Histonet Digest, Vol 90, Issue 27

2011-05-23 Thread Barone, Carol
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, May 22, 2011 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 90, Issue 27

Re: [Histonet] Immuno controls

2011-05-23 Thread Rene J Buesa
Also Sprach Zarathustra René J. From: Carrie Disbrow To: histonet@lists.utsouthwestern.edu Sent: Sunday, May 22, 2011 10:00 PM Subject: [Histonet] Immuno controls Hi all, After consideration I disagree with the consensus and also agree with Curt's pathologist. More supplies and labor wil

[Histonet] In vitro mouse embryo culture

2011-05-23 Thread Carmen Maria Garcia Pascual
Good Moorning to everyone: I am Carmen Garcia a PhD student from Valencia, we are know interesting in make in vitro culture of mouse embryos to do toxicity tests and in our lab we have not experience. someone can give me some advise? or recommend me some bibliography? how long can I have the

[Histonet] This is goodbye

2011-05-23 Thread Margaret Blount
To everyone in histoland, This is my last full week at work. From next Tuesday I will be retiring from my job at Cambridge University. I have thoroughly enjoyed my chosen profession as a research technician specialising in histology. I realise that because I have always worked in research my ex