RE: [Histonet] IHC pos. & neg. control question

This is my understanding about this CAP checklist item - CAP is looking for 2 types of negative controls: 1. Negative reagent control - this is satisfied by running a negative control slide from the same patient block through the whole process without the primary antibody. 2. Negative tissu

[Histonet] PCNA stain

One of our pathologist has ask me to find somewhere to do this stain. I would like to send unstained slides for staining only, he will do the intreperation. Thanks for your help! Pam DeFazio, HT (ASCP) Athens Regional Medical Center Athens, GA ___ Hi

[Histonet] Help me again please

What dilution do you guys use on a rabbit polyclonal Ki-67 antibody...I just did 1/500 and got a crap ton of noise in the areas where the positive staining was. There are definite negative "rivers" flowing through the samples, but even the tonsil control has this same fuzziness to it. Any suggest

[Histonet] Follicular staining of Melan A in lymph nodes

Good afternoon everyone, I am having a strange staining pattern that I have not seen before and I was hoping someone in histo land may be able to help. I am seeing Melan A staining in the follicles only in a sentinel node. There is no background and the staining is cytoplasmic. The node is a

[Histonet] Histology Technologist 2 Job Opening at Northwestern University, Chicago Campus

Histology Technologist 2 Job ID 17253 Northwestern University Chicago Campus This position is for a small Core Laboratory that offers histology service University wide on both campuses and only works with mouse and other rodent tissue. Please review the complete job description requiremen

Re: [Histonet] WEt mount

Instead of getting feedback that could be incomplete or even conflicting, why don't you consul a references book, like Bolles Lee vade-mecum? You will be better off. René J. From: Candice Smoots To: Histonet Sent: Tuesday, May 24, 2011 1:04 PM Subject: [Histonet] WEt mount Hi guys. I am new to

histonet@lists.utsouthwestern.edu

Hello Jim - We too are hoping to purchase a new TEM. I have collected quotes from JOEL, FEI/Phillips, and Hitachi, which range from $295,585(plus the cost of a camera) to $459,960. We did not seriously investigate Zeiss TEMs, as their new generation of scopes is geared towards nanotechnology,

[Histonet] EM purchase

Fortunate enough to purchase and use several TEM and SEM's over the ye= ars, I always found Zeiss to have the best prices and products for TEM and = Hitachi for the SEM's. Last one I bought was 200K plus. Good Luck and= let me know if you know of anyone looking for an EM

[Histonet] part-time position Portland Oregon

A friend has asked me to post an opening for an HT or HTL partime position. It would be about 20 hours per week in Portland, Oregon. If you have an interest, you can contact me via e-mail. kbl...@digestivehlth.com Thank you, Konni Black ___ Histonet

Re: [Histonet] Coronavirus antibody

Hello, We have successfully stained ferret tissue using an antibody to Feline Infectious Peritonitis Virus (which also cross-reacts with swine Transmissible Gastroenteritis Virus). We buy our antibody from Custom Monoclonals, Inc. and use heat-induced epitope retrieval (pressure cooker and t

[Histonet] WEt mount

Hi guys. I am new to this.. Can someone explain to me how to wet mount properly. What is it?  I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Pathology Transmission Electron Micrscope

We are in the market to purchase a new TEM. Our old Hitachi instrument is 31 years old and is getting tired. The use for transmission em in pathology has somewhat diminished in our hospital environment. We still however use TEM routinely on all renal biopsies and nerve biopsies. Has any

[Histonet] Coronavirus antibody

Hi Histonetters, We are interesting in doing immunostain on ferret tissues that have been fixed in formalin and embedded in paraffin. I am just wondering if anyone used this antibody in their lab, if so, can you please share with us your staining protocol? antigen retrieval protocol as well? You

[Histonet] Processor power supply Back-up

Does anyone have any suggestions on power supply back-up for Leica ASP 300?  We are an independant lab and are not hooked up to back-up if we have an outage.  Thought it might be a good idea :)   Thanks. ___ Histonet mailing list Histonet@lists.utsouthw

Re: [Histonet] decal

For tissues containing bone, or bone specimens I always used the commercial brand RDO.  For bone marrow biopsies I used EDTA (prepared in the lab). René J. From: Cynthia Pyse To: 'Histonet' Sent: Tuesday, May 24, 2011 10:56 AM Subject: [Histonet] decal Hello Histonetters What is everyone usin

[Histonet] decal

Hello Histonetters What is everyone using to decal specimens. I haven't had to use it in a few years, before that I just purchased formic acid and made a 10% solution. Just wanted to see if the criteria has changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP)

Re: [Histonet] Re: Freezing bath solutions

Melanie, In our Clini-RF rapid freezer we have a device for keeping tissue submerged in Novec 7000. Regards Alan Bright www.brightinstruments.com Sent from my BlackBerry® wireless device -Original Message- From: mwh...@mcleodhealth.org Sender: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] p63

Biocare has a very good, and fairly inexpensive, p63 that we have had good results with: http://biocare.net/products/antibodies/p/163/ From: Joanne Clark [mailto:jcl...@pcnm.com] Sent: Mon 5/23/2011 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histone

[Histonet] Thank you

Hello everyone! Thank you all for your good wishes on my retirement. I knew you were all great people: you have just proved it big time! Thank you all from the bottom of my heart. Keep smiling! Much love Margaret ___ Histonet mailing