Sara- We stained many tissues for chromogranin and found that the
Churukian stain worked a lot better than the Grimelius. It double
impregnates the cells with the silver. Directions are in Carson's book,
but if you don't have it, I can send them to you.
Cheryl
Cheryl Crowder, BA, HTL(ASCP
The best approach is to increase the fixation time to make sure that the
tissues are really fixed.
Then cut the dehydration times of the lower alcohols by 20% and add that 20% of
saved time as increase in the clearing agent (supposedly xylene or alkane
substitute) times.
René J.
From: Sheila Ad
Please respond to everyone. I would like to have the information also.
Thank you,
Carol
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey
Sent: Friday, June 10, 2011 4:23 PM
To: histonet@lists.
Hello Everyone:
I am looking for a processing schedule for fatty specimens.
Could anyone share theirs?
Thanks in Advance.
:)
Sheila
Bluewater Health
___
Histonet mailing list
Histonet@lists.utsouthwester
I'm wondering which baskets were used (the ones with the spacers?) and which
cassettes (the ones with the solid sides, or slotted?). Does the problem occur
without using these recommended items?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@
Yes, I am also using pH 6 HIER with 10mM sodium citrate, 0.05% Tween-20. I
do 20 minutes at 95 degree C to retrieve BrdU on mouse embryo tissues. I
use Abcam ab6326. Their product sheet states that you may used heat for
antigen retrieval.
The BrdU epitope needs to be revealed by denaturing the dou
Diane:
Under separate cover I am sending you the Grimelius protocol I used (even when
I never worked in New York and even when that is of no relevance!).
René J.
From: "diane.cr...@amcny.org"
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, June 9, 2011 6:43 PM
Subject: [Histonet] Grimelius
We did diif -quik or Alcian Yellow.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison,
Sandra C.
Sent: Friday, June 10, 2011 9:10 AM
To: Setlak, Lisa; histonet@lists.utsouthwestern.edu;
histonet-requ
We do either IHC or a Modified Steiner, depending on the Pathologist's
preference. We switched to Newcomer Supply's Steiner-Chapman Modified
Silver Stain Kit about a year ago, because it eliminated the use of
Uranyl Nitrate. We have been delighted with the consistency of the
stain.
Sandy Harriso
Micromount (Surgipath- now a part of Leica). 21 gauge needle. No problem.
Make sure volume set is correct for the media.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Thursday, June 09,
Good Morning,
University of Arkansas for Medical Sciences is looking for a PRN Histologist
(must be HT registered) for days with an occasional possible night shift.
Please contact me with your information to be considered. E-mail is preferred
as I am rarely in my office. This will be an ongo
Hi All,
Just wanted to know if anyone had any suggestions on how I could improve the
sensitivity of this stain as my last effort was very weak, also does anyone
have any good methods for a chromogranin on mouse tissue.
Kind Regards Sara
---
Hi, I am volunteering in a 300 bed Kenya hospital for a month (Kijabe), and we
have a problem with numerous shiny red artifacts, some of which are the shape
and size of TB (which are very distracting), apparently caused by processing
with 70% alcohol. Does anyone know a way to eliminate these w
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