Re: [Histonet] CE certification

According to the CMP (certification maintenance program) booklet from ASCP http://ascp.org/pdf/CMPBooklet.aspx Page 1: "The Certification Maintenance Program is required for all individuals who became newly certified on or after January 1, 2004 and for all active NCA certificants who are not A

[Histonet] CE certification

Question to all you ASCP rule gurus - I got my certification before the stipulation involving having so many CE's in order to get recertified every 3 years. In order to be "current" with my certification, do I still need to get recertified every 3 years or am I grandfathered in to the old rules

[Histonet] Fwd: Article on the Tornado in the Joplin hospital

A must read article on code Gray. Very sad story but heroic. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Sirius Red Stain

Dear all, Does anyone have a protocol on how to prepare Sirius Red stain with known concentration. We are unsure about which concentration to use. We are trying to stain collagen. The Sirius Red is in powder-form. Thank you. Jesse Hernandez University of Texas - San Antonio Department of Bio

[Histonet] AL state meeting?

Does anyone know if there's an AL state meeting this year? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Larry Farrand is out of the office

I will be out of the office starting 06/22/2011 and will not return until 06/27/2011. I will not have access to e-mail. Please contact customer service with any questions. If you have an Urgent issue that needs to be addressed, Karen Longo my regional manager can be contacted to assist you (ka

Re: [Histonet] Frozen tissue question

I run a Mohs lab that processes skin by frozen section. There is no need to use any fixative before hand. But, if you are looking for melanoma or melanocytes, freezing can cause artifact and make it difficult to read slides. Limit the amount of nitrogen you use to freeze the specimen. Some places u

[Histonet] Removing Paragon/Multistain

Dear all, I was looking for a way to remove my paragon stain from this magnesium implant that had been embedded in methacrylate. It looks pretty dark and I had decided not to use acetone to try to remove it due to the sample possibly cracking. Jesse Hernandez ( Grad Student) University of Texa

Re: [Histonet] Frozen tissue question

Your "new doctor" is absolutely wrong. You do not need to formalin fix or sucrose wash before freezing the specimen in OCT. You can go directly from the fresh specimen → OCT freezing → cutting. You are partially right about the "cross-linking" of the antigens; only "partially" because cross-link

[Histonet] Frozen tissue question

We have a new doctor in our lab who swears that all frozen tissue must be fixed in formalin with a subsequent sucrose treatment before freezing in OCT because not fixing it will cause the structures to be distorted and you can't get good antibody attachment. In my previous experience, we have d

[Histonet] Ventana ISH

I am looking for others who have switched to the new Ventana ISH probes. We are having variable staining and Ventana is unable/unwilling to assist us. We have already cleared up the precipitate and are now looking for reproducibility in the staining. Any help would be appreciated. Thanks Miche