[Histonet] "grossing" again...

2011-06-30 Thread Bruce Gapinski
Is anyone anticipating new CAP changes this year in their lab with respect to "grossing" tissue. We are not in the same site as the doctors and we've been submitting specific surgical specimens for the Pathologist for 20 years. GI bxs, prostate needle bxs, etc. We've also been grossing d

RE: [Histonet] Luciferase

2011-06-30 Thread Connolly, Brett M
All kidding aside, assuming your talking about something tagged with firefly luciferase you can try using monoclonal MA1-16880 from Thermo Fisher. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501

Re: [Histonet] Luciferase

2011-06-30 Thread Angela Bitting
You can, but the retrieval involves a young priest and an old priest. ff McAuliffe 6/30/2011 12:42 PM >>> It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: > ok there is a really BAD religious joke coming soon, isn't there? > > Rene J Buesa 6/30/2011 12:00 PM>>> >

[Histonet] p16 Protocol....

2011-06-30 Thread Tom McNemar
Hello all, I was wondering if anyone would be willing to share a protocol for p16 (Biogenix pre-dilute) antibody on the Benchmark XT. I'm having a little trouble getting anything to stain. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (74

[Histonet] UV decontamination of cryostats in the US

2011-06-30 Thread Jones, Lynne
Hello - I've been following discussion thread regarding a suitable cryostat for use with human specimens. Like Saro (who is in Canada), we also work in a research setting, but primarily with rodent tissues, and only section human tissues intermittently. (We may go for weeks without using our l

[Histonet] Re: Could you please recommend? Cryostats

2011-06-30 Thread Robert Richmond
Amos Brooks observes: >>I have used cryostats clinically as well as in research, and one thing I >>noticed absent from most cryostats is the ability to adjust your block. For >>the life of me I cannot conceive of a reason why a company would have the >>audacity to sell a cryostat that does not a

[Histonet] blade donations

2011-06-30 Thread Bell, Mandy
I just wanted to thank everyone for the overwhelming response we got for microtome blade donations. I think that we are all set now. Thank you so much for your help. Here is a link to a youtube clip of the hospital where our pathologist volunteers if anyone is interested: www.youtube.com/

Re: [Histonet] Luciferase

2011-06-30 Thread Geoff McAuliffe
It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: ok there is a really BAD religious joke coming soon, isn't there? Rene J Buesa 6/30/2011 12:00 PM>>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu,

RE: [Histonet] Benchmark Information

2011-06-30 Thread Rathborne, Toni
You might want to ask for a per diem position, justifying it for use during vacations. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 30, 2011 12:26 PM To: histonet@lists

[Histonet] Cutiing of dextran beads (snap frozen)

2011-06-30 Thread Barone, Carol
HistonettersWe have received some dextran beads (coated with cells) that are "snap frozen"...for cutting. We have done these beads before in histogel/paraffin...but are curious as to how best to handle them snap frozen. We have a nice bead with no cells on our first to snap frozen sample block

Re: [Histonet] Luciferase

2011-06-30 Thread James Hart
Question: Can you stain Luciferase in tissues? Answer: Yes, but the background noise is hell! On Thu, Jun 30, 2011 at 12:23 PM, Tom Wells wrote: > > Question: Can you stain Luciferase in tissues? > Answer: Yes, but it's devilishly hard to do. > > Sorry, I couldn't help myself. > Tom > > Tom Well

Re: [Histonet] Benchmark Information

2011-06-30 Thread Rene J Buesa
According with my experience your complement is adequate for your workload and if I were the one you have to convince to get an additional HT, I would give you a very hard time. René J. --- On Thu, 6/30/11, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Benchmark Informat

[Histonet] Luciferase

2011-06-30 Thread Tom Wells
Question: Can you stain Luciferase in tissues? Answer: Yes, but it's devilishly hard to do. Sorry, I couldn't help myself. Tom Tom Wells BSc, ART Faculty School of Medical Laboratory Sciences British Columbia Institute of Technology Burnaby, BC Canada Phone: 604-412-7594 Cell: 778-228-4102__

RE: [Histonet] Luciferase (OK here it is)

2011-06-30 Thread O'Donnell, Bill
OK here it isYou can stainfor it, but my policy is to only do it if "If the devil makes me do it" (Flip Wilson) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, June 30, 2011

RE: [Histonet] Luciferase

2011-06-30 Thread sgoebel
Someone just responded “only if it’s possessed” I laughed =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Angela Bitting [mailto:akbitt...@geisinger.edu] Sent: Thursday, June

[Histonet] Benchmark Information

2011-06-30 Thread Scott, Allison D
Hello to all in histoland. I am currently trying to find a hospital that is comparable to ours for benchmark info. We a 260 bed county hospital and we are currently processing 10,000 plus cases and have 4 techs,1 supervisor and 1 assistant. If there is anyone comparable to us I would like to hav

RE: [Histonet] Luciferase

2011-06-30 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
We can only hope, LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, June

Re: [Histonet] Luciferase

2011-06-30 Thread Angela Bitting
ok there is a really BAD religious joke coming soon, isn't there? >>> Rene J Buesa 6/30/2011 12:00 PM >>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com Subject:

[Histonet] RE: Luciferase

2011-06-30 Thread Elizabeth Chlipala
You can, we have done this in the past. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504

Re: [Histonet] Luciferase

2011-06-30 Thread Rene J Buesa
You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can yo

[Histonet] Luciferase

2011-06-30 Thread sgoebel
Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwester

[Histonet] Grossing benchmarks

2011-06-30 Thread Rene J Buesa
Read the attachments for the information you have requested. René J. --- On Thu, 6/30/11, aaron...@telus.net wrote: From: aaron...@telus.net Subject: [Histonet] Grossing benchmarks To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 9:59 AM    Histonetters,    I need hel

[Histonet] Grossing benchmarks

2011-06-30 Thread aarondan
Histonetters, I need help finding a reference for the amount of specimens/cases a PA should be able to gross in a day. We use the L4E system and have approxamately 10% Level 5 cases and 10% Level 6 cases. Also, have you found any references for the productivity benchmark

RE: [Histonet] How many tissues an histo tech is suppose to cut per

2011-06-30 Thread Molinari, Betsy
SorryI meant 300 in a 8hr day..including embedding, etc -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, June 30, 2011 6:10 AM To: histonet@lists.utsouthwestern.edu S

RE: [Histonet] How many tissues an histo tech is suppose to cut per

2011-06-30 Thread Molinari, Betsy
I once went to an interview at a research lab , will remain unnamed, and they asked if I could cut 300 /hr. I said "Is this a trick question?" They just stared back. I withdrew my app. LOL Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 83

Re: [Histonet] Could you please recommend? Cryostats.

2011-06-30 Thread Emily Sours
You can't get any better than a Leica 3050. That is top of the line to me. Unless there's some really expensive brand out there I'm not aware of. I love our 3050. I guess your problem is you need a UV lamp, and the 3050 doesn't have one. I would say stick with Leica, their cryostats are awesome