We are using Xylene sulphur free- LR grade( from Rankem, India). I don't
know about its synthetic nature but i will inquire this to manufacture.
Mountant DPX from Merck .
Do you have any suggestion on this combination?
Thanks for all your feed back.
Amita
From:
To:
Cc:
Date: 0
Hi everyone,
Maybe because I had been using the same type all along (low profile) but I was
just wondering what is the technical reason for having the two types of blades?
One cuts better than the other? Price? Longer lasting?
Thank you in advance for your feedback,
Liette Tougas, RT, B.Sc.,
Hi again everyone,
I also wanted to ask if anyone has, or new if there was ever, a regular knife
(not blade) holder for the Reichert Yung 2030 microtome and/or the Leica
microtome series.
thank you again in advance,
Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Da
Are you sure the alcohol covers the slides well? Sounds like water running from
the top of the slide.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-
Make sure the level of the alcohols after the eosin covers the tops of
the slides. It's most likely water dripping down the slides, and this
cause the eosin on the sections to bleed so they looked striped.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[
All very good suggestions and definitely things to consider. Thank you
Jesus.
-Original Message-
From: Jesus Ellin [mailto:jel...@yumaregional.org]
Sent: Thursday, July 07, 2011 10:05 AM
To: 'Carol Bryant'; 'Elizabeth Chlipala'; Rodriguez, Arnold;
histonet@lists.utsouthwestern.edu
Subject
The only problem here is that the Cell Marque antibody is currently on
backorder until at least the end of August from what I've heard.
Mark
On Thu, Jul 7, 2011 at 12:36 PM, Angela Bitting wrote:
> CC1 standard, 32 min incubation with heat disabled. I use Cellmarques
> predilute
> and UltraView
Thank you for the prompt reply. I was also told that the Sakura and
Leica printers are the same. Speed of printing is close to the top of
our least, so thank you for that bit of information as well.
Have a wonderful day.
Arnold Rodriguez, HT (ASCP)
Supervisor Anatomic Pathology
Eisenhowe
Hi,
I would like to know if you finally optimized your staining with the
anti-goat and the open secondary kit. If yes, can you tell me which antibody
and dilution you used?
I'm currently trying to do the same thing here. So far I only tried
secondary antibodies from santa-cruz (the same that we us
We have a website at our hospital that is accessible to all physicians and
nursing staff for collection of all specimens. We call the website LabSource.
> From: trathbo...@somerset-healthcare.com
> To: mpe...@grhs.net; histonet@lists.utsouthwestern.edu
> Date: Thu, 7 Jul 2011 19:36:15 +
>
We have the basic specimen requirements (taken from our procedure manuals) from
each of the Lab areas, and place them in a binder for each of the Nursing
units. So whether it is a fluid or a tissue specimen, or if it requires special
handling or collection, it is in this manual. If something arr
Histology position in Las Vegas, Nevada with national reference lab. The
position is a night shift and includes $5k sign-on bonus as well as
relocation package. Pease contact me for immediate consideration.
Kaitlin Webster
Account Manager
Prometheus Healthcare
Office (301) 693-9057
Cel
Hi All,
Over the past year we have had a recurring problems with our H&E's. The come
out of our Gemini Stainer stripped but not on every run; we can go for months
without a problem and then all of a sudden it is back again. The stripping is
in the Eosin, our pathologist confirms the the hemot
CC1 standard, 32 min incubation with heat disabled. I use Cellmarques predilute
and UltraView DAB detection.
>>> Dorothy Glass 7/7/2011 3:28 PM >>>
Does anyone have a working protocol for pax2 on the Ventana Ultra or XT? Can
you please share on Histonet?
Dorothy Glass
Supervisor, SEPA LABS
Br
Does anyone have a working protocol for pax2 on the Ventana Ultra or XT? Can
you please share on Histonet?
Dorothy Glass
Supervisor, SEPA LABS
Brunswick,Ga.
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You DO have to tell physicians exactly how to collect specimens. They
know how to do their job but they have no idea how we do ours, even if
they did a rotation thru pathology. Sometimes, you may have to tell them
each time they want to send a specimen if they don't do it routinely. We
still
How has everyone handled this question GEN.40104
Instructions are distributed to physicians and paramedical personnel for
proper collection, handling, transportation, and preparation of
cytologic and tissue specimens.
I feel this question is asking me to spell out to a Dr how to collect
his/her
We have been using the Bond III for about 4 months and have starting
experiencing staining on our negative control slide.
We inserted and additional blocking solution and it help for the IHC stains but
we are still experiencing the problem the with insitu.
Has anyone experienced this problem bef
Are you fixing the eyes in Davidson's? We found that using this fixative which
I would have to look up since it has been so long since I did rat eyes helped
maintain better morphology.
> From: himanshu.gu...@ucdenver.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 7 Jul 2011 11:37:39
We process rat eyes all of the time. I know that the collection of the eyes is
important and I can not comment on that since we do not collect the eyes, but
anytime you are handling both mouse or rat eyes for retinal work you need to be
as gentle as possible. For retinal work you need to fix i
Hi
I need an advice from all experts there on histology of Rat eye.
I need to take clear section of eye (cross section) so that I can see each
layer of cells clearly. Please advise me step by step process to prepare the
histology slides/sections of rat eye.
I try it many times but I am not abl
I agree with the previous comments and we are in the process of installing
Vantage. During our testing we found that Ventana will work with any vendor,
the key issue is interfacing to your LIS. As part of the process, we tested
several vendor cassette printers. We chose Thermo's new Printmate
The Sakura and the Leica are the same system as they share a patent (this is
what I am told). We use the Leica IPC for our cassettes and we have the Vantage
system. We did a demo of the Thermo printer but were not happy with the speed
and the fact that we would need to change who we order cas
I do not have Vantage, so I cannot comment on that. But you have to take into
account the environment and the scanning quality of the print. I would also
look at space, location, downtime, workflow, even clarity of barcode meaning
Datamatirx, ect. There are also issues with specific cassette c
We have used Sakura for years now --- cassette and slide. They do a great job.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwe
I am intersted in this info also. I would like to know what printers everyone
are using with the Vantage system or a bar coding system.
Thank you,
Carol
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E
I second Jesus's comments especially if you are moving towards bar coding. You
will need a cassette printer that can print a high quality 2D bar code.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682
I would look at Thermo, Leica and General Data printers .
Jesus Ellin
Yuma Regional Medical Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rodriguez,
Arnold
Sent: Thursday, July 07, 2011 9:38 AM
T
We assist with bone marrows either at bedside or in Outpatient Observation,
never in the OR. All other bone marrows are performed at the Oncologist's
office.
Marcia Fisher
Histology Supervisor/Lab Safety Officer
El Centro Regional Medical Center
1415 Ross Ave
El Centro, CA 92243
760-339-7267
7
Hello All,
We are interested in purchasing a new cassette printer and I would
sincerely appreciate any recommendations for this instrument. We are
particularly looking for reliability, print speed, LIS connectivity and
barcode technology.
Thank you very much.
Arnold Rodriguez, HT (ASCP)
Super
I believe that this could be a multifactorial problem.
There is a tendency for us, "in the interests of efficiency" to process tissue
and slides as rapidly as possible. In a lot of cases I suspect that the
absolute minimum time in reagents causes some carry over that can result in
fading of the
Here is the EM job I recently mentioned would be open. See the HR website at
http://jobs.ucsfmedicalcenter.org/
Job Description
Job ID:
2257
Job Title:
Electron Microscopy Lab Supervisor (HISTOTECHNOLOGIST, SUPVR)
Job Code:
9068
Department:
Pathology-Surgical /
Bouin's and PFA are completely different in their action an results.
If you get good results with Bouin's use it and try to overcome your cutting
difficulties.
There should be no differences using PFA or 37% formaldehyde. Methanol is only
used to prevent formaldehyde polymerization and does not i
If you have an overnight step it is better to prolong the formalin fixation
instead of leaving the tissues in 70EthOL.
Since formalin will require at least 24 hours to completely bind and 48 to
crosslink, if you leave your tissues overnight in 70EthOL the unfixed tissue
will be fixed by the alco
It seems that your mounting medium is acid. Try to correct that rather than
changing the staining.
René J.
--- On Thu, 7/7/11, amitapan...@torrentpharma.com
wrote:
From: amitapan...@torrentpharma.com
Subject: [Histonet] Routine H & E stain,
To: "Histonet" ,
histonet-boun...@lists.utsouthwes
Amita,
We are a neurotoxicology lab that also provides histology services for
other researchers inside & outside Rutgers, and we use Gill's #3
hematoxylin for our routine H&Es on mouse and rat tissues. No fading that
I have noticed in all the years that I have been here. We get ours from
Thermo
Yes we do. We assist with all bone marrows regardles of where they are
(not hematology). We dress out in scrubs, etc.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l
I should also have added not all synthetic Xylenes do this and I also use a
Microwave Processor. These variables may have been a factor. Wonderful world of
Histology, TRIAL AND ERROR.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, C
Are you using synthetic xylene in your H&E/COVER SLIPPING protocol. If yes, you
might want to consider ending your protocol with the real thing (XYLENE). I had
the same situation until we switched to real XYLENE. No more H&E fading.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY
I do, as well as several of my coworkers. We assist with bone marrows in
inpatient rooms, pediatric sedation, outpatient surgery center, and
occasionally in the main OR. What is your question?
Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State
Are there any Histo Techs out there who assist with bone marrows IN the
operating room?
Lisa
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Storing your samples overnight in 70% ethanol after fixation won't harm your
samples, but they should be adequately fixed prior to this. I do not like
xylene for mouse tissues, it makes them too brittle. Instead I used histoclear
11 from National Diagnostics. Histoclear 11 is less expensive than
Thank's,
Did you used it for mouse liver section ?
Can you please post a your fixation protocol ?
2011/7/7 Edwards, Richard E.
> I have no experience of using Bouin’s fixed tissue for immunos, tho’
> it is an excellent fixative for many tinctorial procedures providing the
> fixation time
I had a problem with liver fixation with PFA, most of the time I've got no
signal, so I've switched to Bouin's, and got good resalts, although it is
harder to cut bouin's sections with microtom.
Could it be because that I've switched from Paraformaldehyde powder to
formaldehyde solution (37%) (tha
Dear All,
Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C
during the fixation process, will be better for the tissue fixation, and
does not harm the sample ?
Does the fixation process should be done straight forward from the first
step to the last one without any over ni
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