Hi Randi
I use 0.5% Iodine in 70% Alcohol as a pretreatment to the Warthin-Starry
method to enhance Leptospira, Brachyspira and Lawsonia sp.
I find it works very well.
After bringing sections to deionised water treat with 0.5% Iodine in 70%
Alcohol (prepare fresh) for 5 min.
Treat in 5% Sodium Th
WOW is my bottom lip ever puffy! (Sad face) That thing was awesome. Does
anyone know of anything similar? I had been wanting to get an extra rack
just in case. That reminds me of my favorite Mae West quote "He who
hesitates is a d@mned fool."
Amos
On Tue, Sep 13, 2011 at 4:42 PM, Bea DeBrosse-Ser
It is discontinued. I went on line, and that is what it said.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-bou
I tried ordering this system a few months ago, and it was discontinued?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[m
Hi,
Shandon Sequenza staining racks and slide clips. They are great. You
don't need the whole system (timers & boxes to hold the reagents). Basically
this is a rack that holds slides and slide clips vertically and the reagents
are dropped in at the top of the slide and it displaces the reagent
-Original Message-
From: Carol Fields
Sent: Tuesday, September 13, 2011 4:18 PM
To: 'mad...@verizon.net'
Cc: 'mailto:histonet-boun...@lists.utsouthwestern.edu'
Subject: RE: [Histonet] embedding centers
I bought 3 microtomes from Medite and they would not take them back
(they would swap
You can search it in groups too...it has and H&E heartwell actually
it's some kind of lumen in the shape of a heart. I don't remember the
specific organ =)
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900
Under medical community
How is this site listed? I searched for Histo-Ville, but nothing came up.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
mad...@verizon.net
Sent: Tuesday, September 13, 2011 3:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding centers
I would stay
Good instument, been working with it for about 6 months, using the Fast Flex
system for rapid tissue processing. What did you want to know specifically?
Tim
Message: 8
Date: Tue, 13 Sep 2011 09:11:53 -0700
From: Davide Costanzo
Subject: [Histonet] STP420
To: histonet@lists.utsouthwestern.edu
Me
Daphne,
We do EM work on formalin-fixed, paraffin-embedded tissue occasionally. I
will send you a brief protocol under separate cover.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Av
I agree! We just sent our brand new Medite back to vendor because of problems.
The cold spot was icing over and dispenser was dispensing paraffin when I
wasn't even using it! We just purchased the Sakura tec which has been my
favorite since I've been a tech.
Sent from my iPhone
On Sep 13, 20
I would stay away from Medite. THey are poorly designed and do not
talk to each other. Temps are bad and service even under warranty does
not exist. The embedding mold bin was at the highest temp and still
could not mel the paraffin.Tried to chat with a rep at a conference
Hey there Histonetters!
Does anybody have any feedback regarding the Dako CoverStainer? This is an H&E
stainer with a glass coverslipper attached. We are looking at one and I would
be interested in getting feedback from someone who is using or has used it.
How does it compare to the TissueT
I suspect it will be somewhere between rather ugly to totally worthless,
more likely the latter.
The only was to know is to find out for yourself. Thaw, rinse multiple
times in buffer, osmicate in buffer, rinse, dehydrate, clear and embed
in your favorite epoxy.
Good luck.
Geoff
On 9/13/2011
How is this site listed? I searched for Histo-Ville, but nothing came up.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax
Daphne,
While I can appreciate wanting to try something just to see if it might be
helpful, I really fail to see any benefit from trying to look at ultrastructure
of tissues that have been fixed and stored in this way. The ultrastructure will
be compromised, and it will be difficult to get any
Last October, there was a discussion about being able to bill specials
and Immunos (CPT codes 8312, 88313, 88342, 88360, 88361) per block and
not per specimen. There was a paragraph explaining the change and the
source cited was "CMS /NCCI Updated October 1, 2009." My manager and I
would like to
Hi
I'd like to try some TEM work on rat brain hippocampus. However, the
tissue is less than ideal. Does anyone have any information/insight on
using old (harvested ~2 years ago) tissue that has been subjected to 20
minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
48 hours, a
Final hour - still hoping to find users of the Thermo STP420 to provide me
with some feedback. Any comments on this unit??
Thank you.
--
*David Costanzo, MHS, PA (ASCP)*
Project Manager
*Blufrog Path Lab Solutions*
9401 Wilshire Blvd. Ste 650
Beverly Hills, CA 90212
__
Dear colleagues,
I am bringing your attention to the following job opening at the Stowers
Institute in Kansas City, MO. It is a wonderful place to work, great Principle
Investigators and research staff, fantastic physical location and beautiful
facility. You can read all about it at www.stowers
Hi Histoland,
We are trying to validate the MMR antibodies MLH1, MSH2 and 6 and PMS2. We
have negative control slides. Would anyone be willing to send us positive
controls for MLH1, MSH 2 and 6 and PMS2. I know this is a huge request but
these are very hard to find controls. If you can
If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE on
facebook.
We have over 100 member so far and hoping to expand. Come by and say hi.
Nicole Tatum, HT ASCP
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists
Stephanie,
I prefur the Sakura Tissue-Teks. III being my fav, but it is becomming out
dated and hard to find parts for. V is good to. The Leica brand is always
a winner. Lots of people have these, so there easy to get parts and have
maintenced. Make sure you check and see how many cassettes the w
Hi Leah,
I've done both of these for quite a number of years. You can write me
privately with more questions or requests, if you like.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Av
CC1 standard; antibody 16 minutes @ 37c
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Tuesday, September 13, 2011 9:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histon
Honestly, there are differences ergonomically. We purchased one in the past
before realizing that the way the cassette holding bath was situated you had to
pull the cassettes across the cold plate to get them to the area you needed..
It was very awkward. This unit was also over-engineered wit
As you correctly point out, all are of similar configuration and foot-print.
All use the same technology (a cooling area, a heating bath and a
hot compartment).
As to reliability TissueTek if the best for me. There are some more than 25
years old still providing a good and reliable service.
René
Double ditto. Sakura TEC
~Sean McBride
Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124
412-268-8275 (o)
412-915-1683 (m)
412-268-8275 (fax)
smcbr...@andrew.cmu.edu
On Sep 13, 2011, at 9:40
HEY THANKS HISTONETTEERS
For all your help on controls we have the Ventana benchmark LT, ran the
controls and they are not working maybe we need to tweek our protocol can
anyone help with this ?
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sb
Ditto. Sakura's Tissue-Tek
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org
-Original Message
Sakura's Tissue-Tek
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weaver,
Stephanie
Sent: Tuesday, Sept
Hello histonetters!
I know this has been asked before, but there's not much in the recent archives.
I'd like to see what everyone thinks is now the best paraffin embedding
center. They all seem very similar now, and I don't see any one instrument
that looks much different from the others. My
Ditto the Sequenza
I work almost exclusively with eyes and they wash off the slides easily. The
washes of the automated machines are too rough for them. The Sequenza is all I
use!!!
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK
I love to use the Thermo Shandon Sequenza system. You touch the slides one time
as you place a cover-plate on them and then you just add your reagents at the
top and let gravity and capillary effect do its job. If you would like more
information just contact me directly. Thanks!!!
Kind Regards!
We use the Sequenza immunostaining center available through Thermo. The slides
are inserted upright, with an attached coverplate. This keeps the slides from
drying out and also reduces the amount of reagents needed.
Nancy Thomas
-Original Message-
From: histonet-boun...@lists.utsou
Dear Colleagues,
We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment, we do
everything manually (washing, incubating, ) but I would like to make
things run more smoothly. So my question is, is there an
Hello every Histonet - I could really need your help!
Our lab is currently obsevring pathlogical changes of aortic disease,
especially
elastic fiber and collagen in the background of abdominal aortic aneurysm
(AAA).
The aortic is from C57BL/6N mice.
First, I paid attention to the n
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