RE: [Histonet] Poor quality frozen sections, feline skin.

We fix our tissues first in 4% PFA, wash it 3x 15min each with pbs, then place it directly onto 30% Sucrose to cryoprotect until it sinks -usually over night. I am not sure if the Sucrose helps in the quAlity of the sections, but I have dealt with issues similar to what you are dealing with, mos

Re: [Histonet] Poor quality frozen sections, feline skin.

Hi Peter, We use 20-25% sucrose on our fresh specimens for immunofluorescence (IF). We only deal with human skin samples so I will not comment on feline skin. I sit the specimen in three changes of sucrose for 10 minutes each. Before embedding in the cryostat, I gently blot it on a tissue. My unde

Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

Thanks. Thank God that the company that supplied the product contacted her and set her straight by telling her the same information you guys have told me. I even pointed the chapter from the Frieda Carson's book again, when it clearly says they are the same thing. Now she understood! Thank God I wa

RE: [Histonet] "formol"

Formal is what Dr. Lilklie says, FORMOL is methanal (HCHO). The name started in Germany in 1891 as the trade name for the 37-40% aq. sol. of methanal or formaldehyde. It was adopted in Spain and all Hispano American countries after Dr. Santiago Ramón y Cajal (Nobel Proze in Medicine along with

[Histonet] Poor quality frozen sections, feline skin.

I have some feline skin samples for frozen sections that were sent to us because our client was not able to section them themselves. Unfortunately we are having the same problems trying to make good sections ourselves. The samples seem dry and unsupported when we try to cut them. The tissue brea

[Histonet] Antibodies (IHC) on sheep /goat models

Looking for anti-cytokine antibodies (dete cting IL-1α, IL-1β, IL-6, TNF-α, and TNF-β) and a HAM-56 macrophage marker that react in sheep /goat models. This immunohistochemical analysis would be performed on parafin-embed ded sections and may be outsourced if preferable . Any help/ recommendatio

RE: [Histonet] "formol"

Not to put too fine a point on this interesting discussion (or "lecture series", as it were), I add a little quote from R.D. Lillie, Histopathologic Technic and Practical Histochemistry, 3rd ed, 1965: "The word 'formal' [sic], sometimes erroneously used as a synonym, refers properly to quite anothe

Re: [Histonet] "formol"

ALL laboratories in Spain and ALL laboratories in Hispano America keep the German name for the 10% formaldehyde solution and call it "FORMOL". René J. --- On Thu, 10/6/11, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] "formol" To: histonet@lists.utsouthwestern.edu Date: Thursday, O

[Histonet] open HT/HTL position

HI all, I currently have an opening for a fulltime HT/HTL in Atlantic City. We work Monday through Friday days only, no holidays or weekends.If you know someone who is looking for a position in South Jersey please let them know. This is a modern lab doing aprox. 12K cases/year. I’ve been the superv

[Histonet] Florida Society of Dermatologic Surgeons

The Florida Society for Dermatologic Surgeons will be holding there 30th annual meeting at the Peabody Hotel in Orlando, Fl Dec 2-4, 2011. They are looking for venders or members who are intersted in setting up a booth. The physicians interests are in: Pharmaceutical. Equipment. Pathology labs. Mo

[Histonet] Fontanta Masson on frozen sections

Has anyone performed a Fontana Masson of frozen section skin specimens. If so, would you mind sharing your protocol, especially the fixation step? Thanks, Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/

RE: [Histonet] Tissue left in processor

That is precisely why we take the cassettes out of the molten paraffin (embedding center) if they cannot be embedded right away. I felt they were being "cooked" as well. We have had not problems sectioning cassettes that have hardened and re-melted. Peggy Peggy Sherwood Lab Associate, Photopa

RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4%

Formaldehyde is the concentrated form and formalin the diluted form of formaldehyde. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.ed

[Histonet] ATP 10's for muscle biopsies

I'm having issues with the ATP 10's we run on our muscle biopsies and am hoping those who are familiar with this stain are willing to share their methods?! Any help or advise is greatly appreciated. Thanks Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179

RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4%

I like this article's discussion of the history of usuage and terminology: citation Fox, C., Johnson, F., Whiting, J., and Roller, P. Formaldehyde Fixation, The Journal of Histochemistry and Cytochemistry, Vol. 33, No.8, pp. 845-853, 1985. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu,

RE: [Histonet] Tissue left in processor

Some prefer to take the cassettes off the processor and let them harden until embedding can be done, rather than leave in molten, hot paraffin. This does not damage the tissue, and placing the cooled cassettes into the embedding center holding area allows the paraffin to re-melt in a shorter time b

RE: [Histonet] Floaters

This was an excellent article, I archived it, interesting that your own experiments confirmed this results. Joelle Weaver MAOM, BA, (HTL) ASCP > From: timothy.mor...@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 6 Oct 2011 08:19:25 -0700 > Subject: RE: [Histonet] Floater

[Histonet] CMV/HSV Protocols

Does anyone have protocols for CMV, HSV I, and HSV II (from Cell Marque) that they would be willing to share for the Benchmark Ultra (i-View)? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org | 575 N.

[Histonet] RE: Cutting frozen sections from visceral fat

Hi Sarah, Thank you for the response. We are a research lab so whatever the researchers want to be done we do it with all kinds of tissues. In this case they are looking at the size of the fat cells in the animals. I will try to lower the temperature to -30 as was also suggested to me and if

RE: [Histonet] RE: DAPI on thicker sections, problem with gradient

Hello, Maybe this method was meant to be used on Floating sections instead of mounted sections. That way the binding and penetration would go from both sides of the tissue. Helen L. Fedor http://tmalab.jhmi.edu/ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ma

[Histonet] RE: DAPI on thicker sections, problem with gradient

Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections a

[Histonet] Imidazole buffer

Hi, I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find con

[Histonet] control for Hemoglobin?

Hi Histonetters, What control should I use for Okajima's Stain for hemoglobin? Thank you for your help! Kelly Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University VTPB Histology Lab VMA bldg. 214 College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 _

RE: [Histonet] Tissue left in processor

I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbu...@yahoo.com > To: histonet@lists.utsouthwestern.edu; rchar...@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > >

[Histonet] "formol"

Formol is an old brandname, I think for a desinfection reagent. Not really custom any more. Bye (another) Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Donnerstag,

RE: [Histonet] (no subject)

Very good question Cindi.Jan > Date: Thu, 6 Oct 2011 09:26:18 -0400 > From: robin...@mercyhealth.com > To: mamaw...@hotmail.com; histonet@lists.utsouthwestern.edu; > christiego...@msn.com > Subject: RE: [Histonet] (no subject) > > It is a good thing this vendor does not work in a histo lab beca

RE: [Histonet] Floaters

The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragmen

[Histonet] Ken Marissael is out of the office

I will be out of the office starting 10/06/2011 and will not return until 10/12/2011. I will be away on 10/06 and return 10/12. I will have limited e-mail and cell phone access, but will try to get back to you as quickly as possible. While I am away, please contact VWR Healthcare Customer Servic

RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4%

Now, wait just a minute! Don't tell me that after 40+ years of being a histotech that I've been WRONG all this time??? FORMALDEHYDE is FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this will be my last day at work..

Re: [Histonet] (no subject)

I too spoke to one vendor who claimed that the floaters are not an issue and that their bottles drew below the level of the "floating" floaters. I didn't feel insulted, but did feel that this salesperson obviously had no actual lab experience or they never would have made such a ridiculous claim

SV: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4%

I suppose René meant to say: Formalin is a 37-40% solution of formaldehyde. 10% formalin is a 3,7-4,0% solution of formaldehyde. I've never heard the use of "Formol", but then again, I'm scandinavian, not som much european... ;-) Gudrun, Norway -Opprinnelig melding- Fra: histonet-bou

RE: [Histonet] Tissue left in processor

I don't think you want to leave it in the processor for 2 hours. Even at the embedding center, if someone can't come to help orient the tissue, we then take the cassettes out of the paraffin station and leave on a paper towel. Have done that for sometimes 2-3 days. Peggy Peggy Sherwood Lab A

Re: [Histonet] Tissue left in processor

I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. René J. --- On Thu, 10/6/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] Tissue left in processor To: "Histonet (histonet@

[Histonet] JOB OPENING

Hi All, We are currently searching for a part time person. The Hospital of Central Connecticut at New Britain General has an excellent opportunity for an experienced Histology Technologist to practice in our full service laboratory which consists of hematology, chemistry, microbiology, molecula

[Histonet] Tissue left in processor

Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing

[Histonet] FW: Job Opening

We are looking for a certified HT or HTL, to join our pathology lab in Manitowoc, WI. We currently have two (2) fantastic 1st shift positions open. We are seeking an experienced lab manager for a Monday-Friday position. This would be a salaried position and pay would be commensurate with exp

[Histonet] Floaters

This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the

RE: [Histonet] (no subject)

It is a good thing this vendor does not work in a histo lab because with that comment/attitude he/she would not last long. I have heard the water bath theory but upon investigation this has never been the source because every histotech I work with cleans it each and every time. We have traced fl

Re: [Histonet] Cutting frozen sections from visceral fat

niper if this mail (ID 01FFoF7eL) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=n Forget vote: http://admin.s

[Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

Jenny: Formaldehyde is the GAS methanal that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin"

RE: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

Jenni, I am sending this link that should clarify it to your supervisor, although you are in a difficult position. Yes they are the same thing. Citation:" A fixative labeled as 10% buffered formalin is actually only a 4% solution of formaldehyde. This is because 10% buffered formalin is an ex

RE: [Histonet] (no subject)

I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the p

[Histonet] Cutting frozen sections from visceral fat

Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has e

[Histonet] DAPI question

Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is tha

RE: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

The answer, get another supervisor, she is obviously not up to it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: 06 October 2011 00:04 To: histonet@lists.utsouthwestern.edu Subject: [His