Re: [Histonet] Regarding cartilage and bone staining

Hi Pathik, perhaps you could give us some background on what it is you are researching? On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology < kraniofacialbiol...@gmail.com> wrote: > Hello Histonetters > I am new to this site. I am a clinician and very fresh to research. I was > doing whole mo

[Histonet] Cat Scratch fever

Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: Open - - Histology Supervisor, UC San Francisco

We have just opened the Histology / IHC Supervisor position at UC San Francisco. Apply online at: http://careers.ucsfmedicalcenter.org Search "Histology" to bring up postion. Posting reference number: 2651 Job Title: IPOX Lab Supervisor (HISTOTECHNO

RE: [Histonet] Regarding cartilage and bone staining

You can always decal the sample and process to paraffin and stain for a wide variety of bone and cartilage stains. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.pre

[Histonet] Regarding cartilage and bone staining

Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be us

[Histonet] RE: staining for endothelial glycocalyx

sounds like a job for Lectin Histochemistry Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feathers

Re: [Histonet] Drying oven temps

59 °C, 1h, for all slides. > Is anyone willing to share their slide drying temperature and times? Does > anyone know of a particular standard concerning this? > > Kimberly Artim, AST, HT (ASCP) > Technical Coordinator, Anatomic Pathology > St Lukes Hospital & Health Network > 801 Ostrum Street >

Re: [Histonet] staining for endothelial glycocalyx

On 10/11/2011 2:21 PM, Featherstone, Annette wrote: Does anyone have a staining protocol that will stain sugar structures on the surface of the endothelial cells?? Commonly structures are visable using SEM but I would like to know if it is possible to see it in a paraffin section. Annette

[Histonet] staining for endothelial glycocalyx

Does anyone have a staining protocol that will stain sugar structures on the surface of the endothelial cells?? Commonly structures are visable using SEM but I would like to know if it is possible to see it in a paraffin section. Annette ___ Histonet m

[Histonet] Microwave processor the Histo 5 for rodent tissue

Hello histonetters, I'm wondering if anyone out there doing animal work has used the milestone histos 5 or other instrument to process rodent tissues. I would like your options on the quality. I would be interested to hear if this works well for IHC and bone decaling.. Thanks, Jamie Erickson

[Histonet] Annexin V IHC staining in mouse xenograft

Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue b

[Histonet] RELIA Special Job Alert!! Exciting Opportunities from Coast to Coast 10-11-11

Hi Histonetters!! Coast to Coast the histology field is blossoming again.If you were looking for a new opportunity but the timing wasn’t right or the location wasn’t right or the employers were moving wy t slooowww. Well things are different now!! I am very excited about the position

RE: [Histonet] Cold plate temperatures on embedding station

We calibrate yearly our embedding center both the paraffin chambers and the cold plate, we record temp when used as a part of daily QC. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303)

[Histonet] Cold plate temperatures on embedding station

Hello All,   We just got the Thermo Histostar. Curious to know if there is a CAP requirement for taking the cold plate temperatures on an embedding station? Need to know for quality control purposes. Thank you.   Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital __

[Histonet] Thick cryosections

Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is

Re: [Histonet] 10% sucorse in Sodium Cacodylate

On 10/10/2011 1:37 PM, sarah Tabatabaei wrote: Hi histoneters, I have a question regarding Sodium Cacodylate. In our lab, after fixing our human tissue, we usually leave it in 10% sucrose in double distilled water for a couple of days to remove all the fixatives out of it. Recently, I used a

[Histonet] Drying oven temps

Is anyone willing to share their slide drying temperature and times? Does anyone know of a particular standard concerning this? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital & Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confide

[Histonet] Whole ovary immuno

Good morning: I want to perfom an immunohistochemistry with fluorescence in a mice whole ovary, but I am new in this and in my lab there is nobody with experience in that. If someone has some protocol that I can follow, I would appreciate it. Thank you very much kind regards from Spain ;)