RE: [Histonet] Re: Histostar Embedding Station

2011-10-26 Thread Marcum, Pamela A
We have two. One is about ten months old, the other a month and not a problem with either. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Wednesday, October 26, 2011 2:

[Histonet] Histotech Needed in Naples, FL for Day Shift-M-F (Permanent)-HOT JOB

2011-10-26 Thread Melissa Phelan
Allied Search Partners is currently looking for a qualified histology professional for a full time/permanent position. Location: Naples, FL Schedule: Full Time, 6am-2:30 pm, Monday-Friday. Work one weekend per month. Every 3rd weekend. Requirements: 2 years of formal education beyond HS O

[Histonet] RE: Abs for detecting immune cells in mouse FFPE sections

2011-10-26 Thread Anatoli Gleiberman
CD3 Rabbit monoclonal from Fisher (MA1-39552) works fine in IF on mouse paraffin sections, B220 rat monoclonal from eBioscience (14-0452-85) works fine in IF on mouse paraffin sections. Used both in 1:100 dilution. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High

[Histonet] Abs for detecting immune cells in mouse FFPE sections

2011-10-26 Thread Jan Shivers
CD3 - rabbit polyclonal Ab from ThermoScientific (cat. # RB-360) works on mouse FFPE tissue. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@

[Histonet] Abs for detecting immune cells in mouse FFPE sections

2011-10-26 Thread Yu, Jian
Dear all, It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited

[Histonet] B220 and F4/80 on Ventana Discovery

2011-10-26 Thread Olive Pitt
Hello,   Is anyone using B220 (RA3-6B2) or F4/80 (C1:A3-1) on the Ventana Discovery? Any protocols or tips would be greatly appreciated.  I am new to using Ventanas and would like to use these abs on a Discovery instead of a Dako.  Also, do all antibodies that work on Dako eventually work on the

[Histonet] Re: Histostar Embedding Station

2011-10-26 Thread Tim Higgins
Hey Jessica, We have two Histostar's that have been here since March and have not had any issues with them. Can't say anything bad about them, yet. Thanks, Tim Subject: [Histonet] Histostar Embedding Station To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1319563285.61798.yahoomail...@w

RE: [Histonet] removal me from histo net

2011-10-26 Thread Sherwood, Margaret
To all who want to be removed from the Histonet List--you have to do it yourself! Go to the website and follow the directions. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724

[Histonet] removal me from histo net

2011-10-26 Thread Raymond Stone
Please removal me for histo net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Antibody questions

2011-10-26 Thread Sheila Fonner
Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath specimens, and who is your vendor? Do you like the products, and what platform are you using? Thanks for any information you may have. These are new requests from our pathologist. Sheila __

[Histonet] EBER ISH

2011-10-26 Thread Sheila Fonner
Histonetters, Is anyone out there using the EBER ISH probes from Ventana on the Ultra Platform? I have a few questions. Did you need to get new software installed? Was there any training involved? And do you have successful protocols that you are willing to share? I have asked the quest

Re: [Histonet] saving slides between levels for IHC?

2011-10-26 Thread Kim Donadio
I think it depends on what the specimen is. All of the places Ive worked have always kept extras(levels) on sentinal nodes for keratins, also for prostate needle biopsies for p63/504s or other IHC's.   Really doesnt it come down to what the Pathologist needs to make a diagnosis for the patient?

[Histonet] RE: Please remove my name

2011-10-26 Thread Bea DeBrosse-Serra
If you want to be removed from the list, you have to do it yourself by going to the website below. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.ut

[Histonet] Please remove my name

2011-10-26 Thread Vanessa J. Phelan
Hi, please remove my name from the mailing list. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Please remove my name

2011-10-26 Thread Roger Brown
Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbr...@mst.edu) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Re: Seeking Beta-Amyloid not needing FA pretreatment

2011-10-26 Thread Connolly, Brett M
Interestingwe see APP on frozen sections with 6E10, but not in paraffin sections after citrate HIER. We use the monoclonal from Covance, 1:12000 overnight at 4C. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@me

RE: [Histonet] saving slides between levels for IHC?

2011-10-26 Thread Sheila Fonner
Sandy, I don't think it is a "standard" procedure. However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted. We are a dermatopathology lab. When it is requested, we cut the whole case