Hi,
You can detect NKT cells with CD57. They are also labelled with Granzyme
B too, but I think CD57 will be a bit more specific.
Amos
On Tue, Nov 8, 2011 at 12:32 PM,
wrote:
> Message: 12
> Date: Mon, 7 Nov 2011 09:48:01 +0100 (CET)
> From: "Carmen Maria Garcia Pascual"
> Subject: [Histonet]
In the state of Connecticut, the only agency licensed to handle human tissue or
remains (other than hospitals) is a funeral home. Therefore, our policy is to
only release tissue or remains to a funeral home after the patient or family
has made the appropriate arrangements with them.
Richard
R
I should add, believe includes placental tissue or POC
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: joelle weaver
Date: Tue, 8 Nov 2011 17:42:41
To: ; ;
Subject: RE: [Histonet] micro wave processors
>From my research I have found that the molecular water (polar) c
I should add, believe includes placental tissue or POC
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-Original Message-
From: joelle weaver
Date: Tue, 8 Nov 2011 17:42:41
To: ; ;
Subject: RE: [Histonet] micro wave processors
>From my research I have found that the molecular water (polar) c
CAP retention guidelines of course, but some states have laws allowing the
parents to receive tissue, of course in 70 percent ETOH, for the purposes of
religous rites and burial. Waiver/form needed. This extends/allows for legal
burial rights previously extended to fetuses of further gestation.
Allison Hutton asks:
>>We had a question come up regarding giving patients back their placentas
>>(patient's request) after delivery. Our general rule is not to return
>>tissues, except for religious reasons. We are now trying to come up with a
>>concrete SOP for (or not) returning tissues. I
Yes Victoria, Canadian laboratories can participate in HistoQIP as well.
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada
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Tim Morken notes:
>>I was listening to the Wait Wait Don't Tell Me radio show this weekend and
>>they had to come up with the derivation of "True Blue" as a saying. Turns out
>>it had to do with blue dye colorfastness. Later they would call it Fast Blue
>>- but that does not convey the later me
At most places where I have either served as supervisor or tech, this is what
we did.
Followed the CAP guidelines for retention of wet tissues. Which is 2 weeks
after sign out.
http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_window
Ohio has laws regarding this release- rather recent legislation. This gives
rights to fetal tissue that extends earlier in gestation than previously, and
by my recollection expands upon the legal rights of burial, autopsy provides to
a fetus in previous statutes. I would search your state's rev
Huntington Hospital in Pasadena, CA has a supervisor's position
available immediately. The position oversees Histology and the grossing
room with a total staff of 11. The hospital provides full benefits,
including a 401(b) plan. Huntington is an awesome hospital to work for.
The position sho
I agree that in house developed metrics and benchmarks are always the most
useful. As you pointed out, just productivity measures and metrics without task
analysis and consideration of work flow should be used with some caution. Also
agree that speed pales when it is compromised by errors and a
We had a question come up regarding giving patient's back their placentas
(patient's request) after delivery. Our general rule is not to return tissues,
except for religious reasons. We are now trying to come up with a concrete SOP
for (or not) returning tissues. I was curious what other inst
Like all other things 'histology', blade choice depends on so many different
things! Tissue type, fixation, paraffin type, mircrotome and associated blade
holder, humidity in the workroom, etc. I know there are people who will
poo-poo this opinion--so try it for yourself!
Contact your vendor
Hi Guys-
We know this is a sensitive subject! I realize my response, below, doesn't
answer your question--you'll get the numbers from other Netters. Please be
cautious in using them out of context for your lab and your staffers!!
depend on so many moving parts that in a small lab (like your
67 is too hot for vacuum-pressure assisted conventional processing. MW
processors use up to 84 to evaporate the IPA, but the time periods are often as
little as 5 minutes.
Joelle Weaver MAOM, BA, (HTL) ASCP
http://www.linkedin.com/in/joelleweaver
> Date: Tue, 8 Nov 2011 09:30:13 -0800
> Fro
>From my research I have found that the molecular water (polar) content and
>insulating fat content of the tissue types are added considerations. If you
>look at the physics and how microwaves penetrate and generate heat, this
>really helps in customizing schedules for different tissues and usi
You will always have a recommended protocol. It is up to us as HT/HTL to
determine what exactly will work. That is where TROUBLE SHOOTING falls in.
As I posted earlier, I use a certain type of microwave processor and because
the recommended temps did not work on the type of tissue I was processi
Thanks Gayle for the tip, wouldn't hurt to improve. I will test it and post the
results.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:
This message and
Hi Zoe-
The melting point of your paraffin depends on the paraffin! Each type has a
different additives so the melting point vs high temp point varies and too
much heat can break down the structure. The package should have the correct
set point for the product it contains--generally 3-5 d
I currently use a TBS for a GI lab and my temps for paraffin do not exceed 67 C
during processing. I know it is recommended to be at 84 C, but at that temp it
would fry everything and chatter was a serious issue even after soaking.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CO
You would think that if it's foiled it would be hard to see with the microscope.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Tuesday, November 08, 2011 11:11 AM
To: Cynthia Pyse; Has
I know I've been around a l-o-n-g time, but what the heck is a "foiled"
slide? That's a new one on me! Rats! Foiled again!
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I am in a mirotomy class, and we had a discussion about mircorwave processing.
We need an accurate temperature. Freida states it should be at 84 degrees
however we think she is incorrect. please respond asap. thank you leticia
alvarez
_
Martin
We currently have a Leica CV35030, which is a glass cover slipper. My 15
Pathologists prefer the glass covers slips for the optical quality. We are
required to archive our slides for 20 years, which is another reason we
chose glass cover slips.
The Leica CV5030 if cleaned nightly and mainta
The purpose of getting the paraffin up to 84C is to evaporate out the
Isopropanol that is used instead of Xylene. This does not mean that the
paraffin should be kept at that temperature when it is not being used for the
infiltration step. It should be in an oven or a paraffin pot at a temperat
Dear list members,
we have funding to buy an automated slide stainer and coverslipper and are
considering to go for the Tissue-Tek® Prisma.
If you had the choice: Would you opt for a glass coverslipper or a foil
coverslipper? Feedback of pathologists on the optical quality of the foiled
slides
Because your boss said to leave it at 84 degrees?
Emily
The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson
___
Our paraffin melts at 60C with pressure from a vacuum. I don't know if
there's actually enough pressure to affect the melting point of the
paraffin, but we always check the thermometer hanging inside the oven to
make sure it stays at 60C.
If you have a certain brand you're not sure of, I would che
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