RE: [Histonet] RE: H. Pylori - IHC

We use Biocare antibody with great results on the IntelliPath from Biocare and the Bondmax from Leica. Karen Lahti Arizona Digestive Health -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent:

Re: [Histonet] Paraplast X-tra

I had to believe someone else out there was seeing this too!! Our rep contacted us today and is going to replace it and investigate...I too wonder how this gets past the QC dept., it is super obvious as soon as it starts melting Sent from my HTC on the Now Network from Sprint! - Reply

RE: [Histonet] Paraplast X-tra

We are having problems again too. Have not changed a thing and convinced that it is a bad lot again. Wonder sometimes on the QC that is done on the paraffin. Connie G. > Date: Wed, 16 Nov 2011 13:47:28 -0800 > From: one_angel_sec...@yahoo.com > To: cls71...@sbcglobal.net; histonet@lists.utso

[Histonet] broken chuck holder

Is your tech using anything like ammonia or soap on her ice to soak the blocks?  This would chew through the spring much more quickly than just ice water...??? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time.  281.852.9457 Office 800.756.3309 Ph

[Histonet] microtomes

The settings in a rotary microtome may indicate the thickness of sections but... how do you know that it indeed cuts at the indicated thickness, particularly if it is say an old device that you inherit from somebody else's lab junk? Is there a way of measuring the magnitude of advances after each

[Histonet] AUTO: is out of the office. (returning Wed 10/19/2011)

I am out of the office from Thu 11/17/2011 until Fri 11/18/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mi...@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewe...@leica-micros

Re: [Histonet] Brass cryostat chucks

For what cryostat? On Nov 17, 2011, at 1:37 PM, Goodwin, Diana wrote: > Greeting, Histonetters. > > I am in search of those brass cryostat chucks with the holes in them. Any > ideas? Googled my brains out with no luck. > > > Diana G. Goodwin, BS, HT(ASCP)QIHC > > Department of Pathology

[Histonet] Source of IgG4 for Isotype control

Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Brass cryostat chucks

Greeting, Histonetters. I am in search of those brass cryostat chucks with the holes in them. Any ideas? Googled my brains out with no luck. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton One Hamilton Health Place Hamilton, N

[Histonet] HTL Lead Job Opening

The University of MN Medical Center, Fairview has an exciting and immediate job opportunity as an HTL Technical Lead in Minneapolis, MN. Check out the Fairview web site www.fairview.org and look for job 11-36731. Or contact me, Jennifer Morocho, HTL (ASCP)CM at jmoro.

RE: [Histonet] to cut or not to cut mouse brains

When the blocks are not croyprotected you can cut them at warmer temperatures. But when they are cryoprotected with the 30% sucrose, they are soft at -18, that is why you need the colder temperature. they will compress at the warmer temperatures and give you a lot of wrinkles. Helen -Origi

[Histonet] RE: to cut or not to cut mouse brains

Forgot to mention that we keep the cryostat around -16 to -18 C Brett -Original Message- From: Connolly, Brett M Sent: Thursday, November 17, 2011 2:58 PM To: 'McLaughlin, Terry '; histonet@lists.utsouthwestern.edu Subject: RE: to cut or not to cut mouse brains Terry, Here what works

[Histonet] RE: to cut or not to cut mouse brains

Terry, Here what works for us - Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat. Very gently press the cold slide onto the cut section so it makes contact and the section sticks to the slide. Flip slide over and use your finger to warm the back of the slide und

Re: [Histonet] to cut or not to cut mouse brains

I'm interested in this answer because this has happened to me - but not always. I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and they are usually handled in the same manner before being brought to the lab frozen in OCT. Andi On Nov 17, 2011, at 12:24 PM, McLaughlin,

[Histonet] RE: to cut or not to cut mouse brains

Hello Terry, These are very difficult. The colder the better. (-27). keep the slides in the cryostat. Cut the section. Place section on a cold slide, and keep the slide in the croystat during the following procedure. Hold slide in left hand and brush in right. Place finger under one edge of the

[Histonet] to cut or not to cut mouse brains

Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a

[Histonet] RE: Histonet Digest, Vol 96, Issue 26

In response to the broken chuck holders, I have seen it twice and both times it is with a tech who uses chemicals on blocks (RDO, FORMICAL or AMMONIA WATER ETC) and then not rinse the blocks before putting them on the chuck holder. The chemicals erode the metal mechanism holding the spring and

RE: [Histonet] RE: Validation

So can you do validation backwards? Stain the slides on the new instrument 1st and then stain on the old one to compare instead of looking up old cases to re-run on the new instrument? I have an XT (old) and Ultra (new). From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] Sent: Wednes

[Histonet] RE: IHC

Tonsil is the control we use Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Origi

[Histonet] IHC

So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 W

[Histonet] Manual or parts list for a Lipshaw autopsy table

All, I was hoping that someone could help me locate an owner's manual or parts list for a Lipshaw Model LM-5-A Autopsy Table. It seems like the hydraulics are going out, but we need the manual to try to address the problem. Thanks In Advance, Glen Dawson BS, HT(ASCP) & QIHC Janesville, W

[Histonet] RE: H. Pylori - IHC

We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mai

Re: [Histonet] broken chuck holder

You should observe this tech to see what he/she is doing to break 3 in 2 years. Then you can have a training session in the proper use of the chuck. Either that or let them know that eating spinach before cutting is not a good idea On Thu, Nov 17, 2011 at 6:42 AM, Essex, David wrote: > On

[Histonet] RE: H. Pylori - IHC

We use BioCare's H.Pylori for IHC and it is consistency is excellent. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message--

RE: [Histonet] broken chuck holder

Only when I used to work with the Incredible Hulk. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of yes...@comcast.net Sent: 17 November 2011 11:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet

[Histonet] broken chuck holder

Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsou

[Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing

I just talked to Joel who reminded me. Mouse livers are often hard/ brittle, even under the best of circumstances. Avoid heat when processing, Room temp only Gradual dehydration 50/50/70/95% alc. 1 Hour per station. Steve 631 361 4000 ___ Histonet mail

[Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other methods

Not appropriate for your current mamafufu, But here are two 're-processing' methods used in our lab for small fixed tissues. Method 1 where the tissue was bit too large for a short processing cycle and the center of the block is sub-optimally infiltrated or soft and needs more paraffin time. C

[Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing

This sounds like a complicated problem- Generally fixed tissues left in the processor overnight can just be re-hydrated in formalin for a few hours and then run (we've made that mistake) Exceptions abound in histology methods, but Unless the tissue was well-fixed to begin with, Re-processing ge

RE: [Histonet] Fatty mamma tissue keeps on washing off

Hi histonetters Here is an update on my struggle to keep the fatty mamma tissue on the slides. I got a few responses on my mail so I have tried a few other things. Post fix the slides. Deparaffinize the slides using xylene and hydrate to water, post fix the slides in 10% NBF for 10 minutes