[Histonet] IF on lung tissue

2011-12-07 Thread Richard Cartun
Does anyone still do direct immunofluorescence on frozen lung tissue looking for immune-complexes, and do you find it helpful clinically? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Patho

[Histonet] Re: Histonet Digest, Vol 97, Issue 11

2011-12-07 Thread Matt Mincer
Hey Eric, We make a universal, ergonomic microtome handle. You can find it at our site or from Newcomer Supply. http://www.newcomersupply.com/products/laboratory-equipment http://store.techonebiomedical.com/store/products/histo-comfort-grip.html Hope this helps. If you have any questions, fee

[Histonet] Leica/Jung 2030 Microtome Ergonomic Handle Extension

2011-12-07 Thread Matt Mincer
Hey Eric, We make a universal, ergonomic microtome handle. You can find it at our site or from Newcomer supply. http://www.newcomersupply.com/products/laboratory-equipment http://store.techonebiomedical.com/store/products/histo-comfort-grip.html Hope this helps. If you have any questions, fee

Re: [Histonet] Histo Aide Duties

2011-12-07 Thread Kim Donadio
I'm sure there are worse comments that could have been made to your "opinion" that someone should be licensed to coverslip. Obviously you realize the cost for labs for such an act? Coverslipping is not technical in my opinion. (or CLIA's and CAP's )   Yes, supervisors are mandated with the respo

Re: [Histonet] RE: Histology lab assistant

2011-12-07 Thread joelle weaver
This sounds like an ideal mix. Thanks for sharing-all people can help for sure. I appreciate that you expect people to strive for certification, still think that is important to the future field as a whole moving forward. Sent from my Verizon Wireless BlackBerry -Original Message- From:

[Histonet] RE: Histology lab assistant

2011-12-07 Thread D'Attilio, Shelley
Hi all, Matt asked about the duties of a non-certified Histology aide. We have had this position for about 4 years and I consider it absolutely vital to the running of the lab. We originally started with a half-time assistant working in the afternoons (the time with the busiest call volume).

[Histonet] Looking for Brain Tissue with MS plaques

2011-12-07 Thread Bull, Jennifer L.
I'm looking for brain tissue containing ms plaques for use as a control for the Luxol Fast Blue Stain. Would anyone out there be willing to part with a block or know of somewhere I can order such control slides? Thanks for your help! Jenny mailgate.hinet.org made the following annotations --

[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.

2011-12-07 Thread Diane . Tokugawa
I will be out of the office starting 12/07/2011 and will not return until 12/08/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Client services 8-421-5408, Maria (5408), Mario at 8-421-4961,

RE: [Histonet] LILLIE

2011-12-07 Thread WILLIAM DESALVO
No worries and no fast one assumed. There is no issue w/ anyone trying to create value and get what they can for a valuable item. The book, who offers the book and it's use will always provide value to the recipient. I stand by my previous statement -This book is a must for all Histology labs!

[Histonet] RE: Histonet Digest, Vol 97, Issue 9

2011-12-07 Thread Foshey, Annette
Histonetters: I'm looking for a pediatric institution that charges for autopsy services. What is the charge for a routine pediatric autopsy? What is your charge for a complex autopsy? Thanks in advance for your responses, Annette Foshey, HT Histology Department Children's Hospital of Wisconsi

RE: [Histonet] Histo Aide Duties

2011-12-07 Thread Rene J Buesa
I think that using the qualifier "silly" is not called for. Regardless of how things were done years ago it does not mean that they ought to continue that way. As the last step I personally think (even if you think it is "silly") that cover-slipping should NOT be completed by an unlicensed person

RE: [Histonet] Histo Aide Duties

2011-12-07 Thread Nicole Tatum
Most professional labs require a person who has completed an accrediated histology programs, but CLIA states that a person can work within a histology lab if they have a combination of 12hrs of college chemistry or biology. In any combination. Then that person needs to be trained in each area they

Re: [Histonet] Histo Aide Duties

2011-12-07 Thread Kim Donadio
I guess my first reply was too confusing. It's a confusing subject when your dealing with multiple states to which some are goverened by a requirement for a state license and some are not.   Hopfully this will clear it up or muddy it even more, not sure.   Labs that are accredited and get  reimb

[Histonet] Leica/Jung 2030 Microtome Ergonomic Handle Extension

2011-12-07 Thread Gagnon, Eric
I'm looking for vendors who sell microtome handle extensions (vendors welcome to respond to list or directly to me). Has anyone purchased these? I'm looking for contact information and details/costs. We need to make microtomy as ergonomic as possible for microtomists who have difficulty movi

[Histonet] RE: Tissue Loss

2011-12-07 Thread Rathborne, Toni
Is there water left under the sections before they go into the oven? Are they left in the oven they same length as the other techs slides? I remember having a seasoned tech that was put off by questioning also. I tried a different approach by meeting with her privately and discussing the matter.

[Histonet] Histotechnician Needed for the 3rd Shift in New York, NY

2011-12-07 Thread Brannon Owens
Allied Search Partners has an opening for a qualified applicant to work for a large organization in New York City. We are inviting the best of the best in the field of histology to apply for this unique opportunity! Position: Histotech Shift: Monday-Friday 12am (midnight) ­ 8am. No Weekends! Pe

[Histonet] RE: Histonet Digest, Vol 97, Issue 9

2011-12-07 Thread Mayer,Toysha N
Deanne, When using automation, you should always keep a short supply of your most requested reagents for manual staining on hand. You just never know when the instrument will break down. Automation is great in the long run, in the beginning though you may spend a bit more than expected, just bec

RE: [Histonet] Histo Aide Duties

2011-12-07 Thread joelle weaver
I don't want to seem nit picky, but I tend to strongly agree with Rene's point about coverslipping. It is not really the act or task of coverslipping to me, but rather the fact that it is one of the last opportunities to assess the slide for technical quality, overall presentation, and informat

RE: [Histonet] Lillie, 3rd Edition

2011-12-07 Thread Blazek, Linda
That alone is worth her weight in gold. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, December 07, 2011 12:39 PM To: WILLIAM DESALVO Cc: histonet Subject: Re: [Histonet] Lil

[Histonet] Re: Lillie, 3rd Edition

2011-12-07 Thread Bob Richmond
You'll get my copy of the third edition of Ralph D. Lillie's Histologic Technic and Practical Histochemistry when they pry it from my cold dead fingers. I wish I'd bought the fourth edition, late 1970's I think. It's a great rarity and I've seen very high prices for it. Bob Richmond Samurai Patho

[Histonet] lab chairs

2011-12-07 Thread Amber McKenzie
Where do you recommend buying lab chairs? I'm looking for some w/o arm rests, solid black or tan, on rollers, etc.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] LILLIE

2011-12-07 Thread Breeden, Sara
Well, you Crafty Individuals who found a 3rd edition Lillie at Amazon or the like and for cheaper, make it necessary for me to withdraw my offer of sale. I can't sell it for $8 and I'm not sure even about $40 (those online prices). No hard feelings. Deal's off. And I wasn't trying to pull a fa

Re: [Histonet] Lillie, 3rd Edition

2011-12-07 Thread Victor Tobias
But they're not autographed by you know who. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-744-2735 206-744-8240 Fax ==

RE: [Histonet] Coverslipper

2011-12-07 Thread Rathborne, Toni
StatLab coverglass. It also comes in a 2oz size which is nice for the coverglass dispensers on the CV5030 that we have. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Wednesday, December

RE: [Histonet] Looking for a very good refurbished rotary microtome to purchase.

2011-12-07 Thread Ken Piech
Check the Microtome category on LabX, we are a marketplace just like eBay but for lab professionals wanting to purchase new and used lab equipment mainly. http://www.labx.com/v2/newad.cfm?catID=39 You can post a free Equipment Wanted ad as well. Best Regards, Ken Piech www.labx.com -Origina

RE: [Histonet] Lillie, 3rd Edition

2011-12-07 Thread WILLIAM DESALVO
I just checked and Amazon has used, good condition, hard cover for $6.00. No matter the cost, the book is priceless if you work in a Histology lab. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 7 Dec 2011 12:14:51 -0500 > From: mcaul...@umdnj.edu > To: histonet@lists.utsouthwestern.edu > Sub

[Histonet] Coverslipper

2011-12-07 Thread Marcia Funk
Hello from the North land- We are having coverslipper issues with our Lecia. We have noticed that they coverslipes are not coming in clean as before and dropping off the

RE: [Histonet] Histo Aide Duties

2011-12-07 Thread Mandy O'Connor
Hi guys and gals, I have to comment on this subject. This is where I wish there was a clear cut answer in deciphering what duties an aide vs. HT vs. HTL should and shouldn't do. I work in a private pathology lab where we have a little of everything-an HTL with BS in science, an HT with a 2 yr,

Re: [Histonet] Lillie, 3rd Edition

2011-12-07 Thread Geoff McAuliffe
I wonder what my 4th edition (1976) is worth? Geoff On 12/7/2011 10:27 AM, Breeden, Sara wrote: In honor of my impending RETIREMENT on April 1, 2012, I have decided to offer for sale my copy of Histopathologic Technic and Practical Histochemistry by R.D. Lillie, 3rd edition (1964). It is compl

Re: [Histonet] Histo Aide Duties

2011-12-07 Thread Rene J Buesa
Cover-slipping involves several decisions about size of the covers-lip, cleaning excess mounting medium and the like. Is the final step in the preparation of the slide and could also include a quality control about the staining that could result in returning the slide to re-stain. That type of d

Re: [Histonet] adding eosin to tissue

2011-12-07 Thread V. Neubert
I cannot see cons right now, but histoland: please feel free to correct me. Eosin is soluble in water, and as dewaxing most certainly will end in water, the eosin will be washed out by 70% EtOH and distilled water. Check your eosin to be sure. Am 07.12.2011 17:17, schrieb Carol Bryant: > What ar

Re: [Histonet] Histo Aide Duties

2011-12-07 Thread SHANE NELSON
Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts.   HAPPY HOLIDAYS,   PATTI RUBEN-NELSON  H.T.(ASCP) SUPERVISOR/DGC P.O. BOX 412 CABAZON,

RE: [Histonet] Histo Aide Duties

2011-12-07 Thread Nails, Felton
This sounds like union rules not CAP rules -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 07, 2011 9:55 AM To: histonet@lists.utsouthwestern.edu; histonet-requ...@l

[Histonet] adding eosin to tissue

2011-12-07 Thread Carol Bryant
What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com

[Histonet] IL-5

2011-12-07 Thread angela smith
Does anyone know of a contract lab or facility that is performing IL-5 IHC on paraffin embedded tissue   I have contacted US Labs, Labcorp and Genzyme and they do not. Thank you Angela Smith Histology Supervisor Children's Mercy Hospital   ___ Histonet

Re: [Histonet] elastic fiber stain in dermis of skin

2011-12-07 Thread Rene J Buesa
Use the van Gieson stain. Just make sure that ALL the reagents are fresh (specially the iron hematoxylin). Try ARTERY as a (+) control. René J. --- On Wed, 12/7/11, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] elastic fiber stain in dermis of skin To: histonet

Re: [Histonet] Histo Aide Duties

2011-12-07 Thread Rene J Buesa
Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with

[Histonet] elastic fiber stain in dermis of skin

2011-12-07 Thread Kalleberg, Kristopher
I am currently running a study to determine the increase in elastin in the dermis of skin due to certain treatments applied to skin. These samples will be FFPE after the study concludes. Does anyone have suggestions on which type of elastin stain works best for FFPE skin samples? Are there any c

Re: [Histonet] Non-Certified Histo Aide

2011-12-07 Thread Kim Donadio
You're probaly going to get a variety response to this depending on the state the person is in. Remember that CAP is an acreditation organization made up of peers that help each other stay within the guidlines of other state or federal regulatory agencies, such as CLIA and AHCA. < My version of

[Histonet] Lillie, 3rd Edition

2011-12-07 Thread Breeden, Sara
In honor of my impending RETIREMENT on April 1, 2012, I have decided to offer for sale my copy of Histopathologic Technic and Practical Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and in very good condition; pages unbent, minor page darkening (age) and comes with a FREE autog

[Histonet] Non-Certified Histo Aide

2011-12-07 Thread Matthew Lunetta
Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to kn

[Histonet] Histo Aide Duties

2011-12-07 Thread Matthew Lunetta
Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to kn