RE: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain

2011-12-08 Thread Connolly, Brett M
Doug, Hopefully you are using the CryoJane slides (not the subbed slides) and the UV glue activator and keeping both slides and tape cold in the cryostat. Be aware that you can get CryoJane slides with different amounts of adhesive (0.5-4x). I would suggest going to the 4x slides. When peeling

Re: [Histonet] pathology grossing stations

2011-12-08 Thread Joe Nocito
Bob, try Mopec. When I was at this year's NSH in Cinci, they had a few different sizes and configurations. Joe the Toe - Original Message - From: "Bob Richmond" To: "Histonet@lists.utsouthwestern.edu" Sent: Thursday, December 08, 2011 2:19 PM Subject: [Histonet] pathology grossing s

[Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain

2011-12-08 Thread Douglas M Burns
Hello, Histo-users, Follow-up to our original posting about fixed-brain cryosections falling off of subbed slides. We fished out our Cryo-Jane apparatus, and using this apparatus has resulted in a great improvement in sections and getting sections stuck onto the slides. This works pretty well

[Histonet] MOHS Histotech Needed in Dover, DE

2011-12-08 Thread Brannon Owens
Allied Search Partners is looking for a qualified professional to fill the below position. Position Title: MOHS Histotech Schedule: Full Time, Monday-Friday, Day Shift. This is a permanent/long term position. Location: Dover, DE Summary: · Perform Histology and Cytology preparation proc

RE: [Histonet] pathology grossing stations

2011-12-08 Thread Rathborne, Toni
Check the Mopec website ( www.mopec.com ). They have a nice selection of stand-alone and counter-top models that use filters and do not have to be vented to the outside. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

[Histonet] pathology grossing stations

2011-12-08 Thread Bob Richmond
A laboratory I'm doing some work for is planning to remodel within two months. We see about 5,000 specimens a year, with a moderate number of hospital major specimens including placentas. Currently we have no grossing station, and this may be an opportunity to get one. It will be installed in a win

[Histonet] Histology (MOHS) Technician Needed in Georgetown, TX

2011-12-08 Thread Brannon Owens
Allied Search Partners is searching for a qualified MOHS Technician (Histology) for a laboratory in Central Texas. Location: Georgetown, TX. Just North of Austin, TX. Shift: Full Time. Monday-Friday. This is a permanent/long term position. Duties & Responsibilities: · Assist MOHS Surgeo

[Histonet] Tissue processors

2011-12-08 Thread Bilger, Andrea
Histonetters, Do any of you have any experience with the Lieca Peloris II tissue processor? Andrea Bilger Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally p

[Histonet] RE: lab chairs

2011-12-08 Thread Knutson, Deanne
Amber, We recently purchased chairs from VWR International LLC and are pleased with them. They are solid black, no arm rests, have adjustments for ergonomic settings, on rollers, etc The catalog numbers are 80086-438 and 80086-440 if you want to look them up on their website. The price w

[Histonet] RE: lab chairs

2011-12-08 Thread Goins, Tresa
We use backless drafting stools made by Office Star ordered from www.cymax.com. The saddle seat gets funny looks but we wouldn't use anything else now - they roll, tilt, go up and down and eliminate the pressure on the thighs from the front edge of a regular chair. -Original Message-

[Histonet] RE: EM fixative composition

2011-12-08 Thread Hawkins, Hal K.
McDowell-Trump fixative is an inexpensive formulation of glutaraldehyde and formaldehyde and is available ready-made from EMS Diasum. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of idimi...@mun.c

RE: [Histonet] RE: EM fixative composition

2011-12-08 Thread Sherwood, Margaret
We are a research lab, but we use K2 (Karnovsky's fix) as well. Since paraformaldehyde is hazardous (and a bit involved to make up), we simply buy the glutaraldehyde and paraformaldehyde in sealed ampules and make it up in a 0.1M cacodylate buffer. We also check the pH (7.4), not the osmolality.

RE: [Histonet] Histo Aide Duties

2011-12-08 Thread Sheila Fonner
WELL SAID!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Wednesday, December 07, 2011 5:47 PM To: Rene J Buesa; joelle weaver; histonet@lists.utsouthwestern.edu; Nicole Tatum Subject:

[Histonet] RE: EM fixative composition

2011-12-08 Thread idimitro
Hi Ronnie, For EM on clinical samples we use Karnovsky's fixative, which is a glutaraldehyde/paraformaldehyde solution in 0.1M cacodylate buffer. We prepare our own but you can buy it. We found it does the best job in preserving the cell organelles and membranes. We do not check the osmolality

[Histonet] EM fixative composition

2011-12-08 Thread Houston, Ronald
Trying to get a sense of how people are making up their glutaradelhyde for EM on clinical specimens. Is anyone buying it in premade, and if so from whom? What buffer system are you using? phosphate or cacodylate (or something else)? Are you checking the osmolality of the fixative before use? Tha

[Histonet] ASCP Membership

2011-12-08 Thread Diana Martinez-Longoria
Hello, Thank you to all that responded to my question regarding ASCP membership, now I can make my decision. Have a great Merry Christmas! Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongo...@ecrmc.org

[Histonet] pharmacia

2011-12-08 Thread Edwards, Richard E.
And a related question, who is distributing Pharmacia's antibodies at the present?, many thanks. -Original Message- From: Edwards, Richard E. Sent: 08 December 2011 10:47 To: Histonet Subject: antibody to eosinophil EG2 Looking for a supplier of the above antibody, I am aware that P

[Histonet] antibody to eosinophil EG2

2011-12-08 Thread Edwards, Richard E.
Looking for a supplier of the above antibody, I am aware that Pharmacia did supply it at one time, but contacting them to date has been fruitless, many thanks. Cheers Richard Edwards Leicester Univ