Re: [Histonet] difficult cross section to cut

Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at

Re: [Histonet] Quality Management Monitors

On this specific function I've always used a form that goes out with the first few trays. On that form about an Average of 10% daily cases is picked from these first few trays. The sheet ask. Microtomy good or poor with a comment area for cases chosen as poor.has who cut it as well The pathologi

[Histonet] out of office today

I will be out of the office starting 01/10/2012 and will not return until 01/11/2012. I will be at a meeting at the Harbor City facility so will be on cell phone if needed. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cel

Re: [Histonet] bromoform ingestion

What a interesting subject. I'm afraid I have no answer for you but wow did I go from whooping cough to drinking water to sea weed all the way to the desalinization of the artic on this chemical compound. I hope someone else has your answer. For me. I just want to thank you for a good subject to

[Histonet] Cryostat Temp settings?

Hi, We are currently sectioning mostly mouse lung and liver tissue embedded in OCT. Our cryostat temp. settings are as follows: 1. OT -13°C 2. CT -17°C These temperatures were set during initial setup of the instrument by the field tech. Our cutting is ok (obviously, lung is a bit more pro

[Histonet] CD marker help [particularly CD68]

Hi, It sounds like there are a number of antibodies with similar problems here if you are having troubles with CD68, F4/80 and CD11b. If you are getting variable results it could be that the tissues are not fixed and processed sufficiently. If they are not fixed sufficiently there will be incon

Re: [Histonet] Sodium Barbital

There is a substitute that has been around for a long time. I have used this substitute for immunoelectrophoresis and gotten good results. The reference is Clin Chem, 1978, 24(10):1825-1827. I can send a copy if you don't have access to the on-line journal. You might also search the archives.

[Histonet] Used Tissue Embedding Station

Hello Histonetters, I am looking for a used Tissue Embedding Station.If anyone is interested in selling ,kindly contact me. Thank You Regards Sowmya Kedarnath ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mai

Re: [Histonet] Quality Management Monitors

I have used QA feedback/documentation/randomized block and slide audit. Would include occurence, root cause, technical variables, patient impact, correction, training, competency etc. I would think you develop scaling like FMEA maybe for subjective items. The pathologist feedback is always a goo

Re: [Histonet] RE: Sodium Barbital

Tried Sigma-Aldrich/Fluka? /D On Tue, Jan 10, 2012 at 11:02 PM, John Shelley wrote: > Hi Carol, > > I know that a PI here has been able to get sodium barbital from Premier > Pharmaceutical Labs. I do not have information but I am sure you can Google > it. Hope this helps! > > Kind Regards! > >

[Histonet] Quality Management Monitors

In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality o

[Histonet] RE: Sodium Barbital

Hi Carol, I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! Kind Regards!   John J Shelley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:

[Histonet] Sodium Barbital

Histonetters, Anyone out there still doing ATPase's on muscles these days? Where are you ordering your sodium barbital from? We have always used Spectrum. They no longer carry it. If we cannot find another vendor, is there an alternate protocol someone might share with us? Thanks guys!

[Histonet] bleaching melanin

Hello Histonetters I need to bleach melanin out of some paraffin sections. The only bleaching solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2 solution for 1 to 2 days. How long do you think it will take to bleach the section in a 3% solution? Of course everyone wants it

[Histonet] (no subject)

http://dickcolewatercolors.com/images/3rd-level-pages/xmas.php?sound128.jpg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Digest users please read

Thank you thank you! Have a great day everyone! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] difficult cross section to cut

Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10º, go to 30º angle. René J. --- On Tue, 1/10/12, DiCarlo, Margaret wrote: From: DiCarlo, Margaret Subject: [Histonet] difficult cross s

[Histonet] Position Opening: Clinical laboratory based in the Trumbull, CT area seeking an experienced Histotechnician

Fine clinical laboratory based in the Trumbull, CT area currently seeking an experienced Histotechnician to perform routine & non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s). The shift is Monday – Friday, 8a-5p. Accountabilities wil

[Histonet] stripping antibodies

Anyone know a buffer or method (not using heat) to strip antibodies off cells used in an immunocytostaining procedure? The cells are in a multi-well tissue culture dish and have only been labeled with primary (unconjugated) antibody. Thanks. Merced M Leiker Cardiovascular Medicine Biomedical Re

[Histonet] RELIA HOT HOT HOT Histology Job Alert - 01/10/2012 Run your own private speciality lab in Atlanta, GA

Hi Histonetters! I hope everyone is having a great day! I have a new position that I am pretty excited about. This opportunity is with a private specialty lab in Atlanta, GA. This is a full time permanent position and my client offers excellent pay and benefits. They are looking for someone who

[Histonet] difficult cross section to cut

Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a

[Histonet] Cryostats

Hi Everyone,   We are looking for a new cryostat and are wondering what everyone has, how they like them, what they recommend, etc! Thank you,   Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsou

[Histonet] CD marker help [particularly CD68]

Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I am focusing on the CD68 for now)on some mouse adipose tissue and have been getting varying results. I don't have any experience working with CD marker or doing IHC on adipose tissue. Without getting into the whole long proc

[Histonet] Test

Hmm Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/li