[Histonet] LCM on Paxgene fixed tissue

Does anyone have any experience working with paxgene fixed tissue on membrane slides for LCM? If so - how do you deal with tissue adherence without heating slides longer. Thx Kim Donadio wrote: Oops. 10% bleach to clean. They do have some stuff called tuberculicide < spelled wrong I'm sur

RE: [Histonet] EPHI

That are strict guidelines given for this on the HIPPA website. I know because somehow I was delegated to write the procedure. Just check out their website and look at "physical safeguards". Kaye Ryan North Florida Dermatology Associates, PA Lab Manager -Original Message- From: histonet

[Histonet] 2012 California Society for Histotechnology Symposium in San Diego

Subject: CSH Meeting May 3-6   This year’s meeting will be at the Bahia resort hotel on Mission Bay.  2 Blocks from the Ocean.  We are Presently lining up an outstanding list of lecturers.  Classes will be held on 2 paddle wheel boats.  Take a look at the hotel website and start planning now.  D

[Histonet] EPHI

I'm wondering what others do when selling used instruments that contain EPHI (electronic protected health information). Do you destroy the hard drive which also has the software programs, or is there another alternative? I have an old Dako Autostainer that we have replaced and would like to sel

[Histonet] Re: Efficacity of fluorochrome in Immunofluorescence

Hi Natalia, What type of tissue are you working with, and how was it processed? Are these paraffin sections or cryosections? What fixative did you use? These details can help narrow down a potential problem. If you post your protocol that will help. You might be seeing autofluorescence from the t

RE: [Histonet] OCT embedded frozen tissue for paraffin embedding

Yes, just wash out the OCT and fix as normal...Just remember that these tissues might not look the same as normal fixed tissues because they were frozen first. Sometimes you can get cracking or freezing artifact. Good Luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutic

Re: [Histonet] OCT embedded frozen tissue for paraffin embedding

One of our investigators wants to take his frozen tissue (embedded in OCT) and now fix in formalin for paraffin embedding. I assume he needs to wash out the OCT (?phosphate buffer that formalin is prepared in) and then fix in 10% formalin. I would appreciate feedback from the listers. Thanks! Pe

RE: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently.

Thanks for the advice! We have been worried that wax was the issue. I do leave them in 3 sets of xylene for 5 minutes each, you would think that would get rid of the wax. Is it ok to leave them in the xylenes for longer than 5 minutes each? Thanks again for the advice to everyone who resp

Re: [Histonet] Efficacity of fluorochrome in Immunofluorescence

You are likely getting some cross-reactivity with your secondary antibodies (one of them may be crossing to the other primary).  We always run a battery of control slides when validating a double-IF stain.  Run these controls and you will likely figure out where the problem is:   Slide Set Anti

[Histonet] job opening

Arkansas Children's Hospital has a position for a full time histology tech. This is a 8:30 am until 5:00 pm shift Monday through Friday. We are a small friendly lab who works well together. ACH offers excellent benefits. Apply online at www.archildrens.org Haze

[Histonet] (no subject)

Hello All, I have completed my 2011 statistical analysis of our ER/PR results, comparing them to 'published benchmarks' as per CAP/ASCO guidelines. Has anyone also performed this for their Her2 testing? What benchmarks did you use? Can you site your source? We are currently just running Her2 FISH.

[Histonet] MCP antibody?

Good morning everyone, Does anyone use MCP antibody (Merkel Cell Polyoma Virus) from a vendor other than Santa Cruz Biotech? If so, would you please let me know your source? Thank you! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248

[Histonet] Dermatology software

HI, We are beginning to look at a computer program for our private Dermatology organization and I was wondering if you would share your ideas on a good derm program to explore? We have five offices in our organization but only one lab and right now we do all accessioning by hand. I am looking fo

[Histonet] Efficacity of fluorochrome in Immunofluorescence

Hi everybody! Maybe you can help me. I'm trying to do immunofluorescences to see the colocalisation between two proteins (PSD + Oligomeres) using Oregon Green (goat anti mouse) and Texas Red (goat anti rabbit). But my probleme, is that the analysis of the slides reveals too much colocalisa