[Histonet] endogeneous peroxidase blocking

Morning All Posing this question for a colleague. Suggestions for immunohistochemistry blocking for endogeneous peroxidase probably caused by red blood cells in mouse lung tissue. Using 1% hydrogen peroxide/dist.water for 15mins and still getting non specific staining. Thank you Marilyn

RE: [Histonet] (no subject)

The reason for using diastase is to remove the glycogen from the tissues. AND if you do not heat the methenamine silver solution for the GMS it will take forever to stain, in fact all you will get is background spots. This I can verify, have you ever forgot to turn on the water bath before stain

[Histonet] New DNA Probes for Kappa and Lambda

In the past we have used a cocktail DNA probe (ISH) for Kappa and Lambda. Has anybody had any experience validating and setting up the new probes from Ventana which we have to mix the four probes together and form our own cocktail? Please share with me your thoughts. I am seriously consideri

[Histonet] QA by pathologists

Akemi Allison asks: >>What % QA do most pathologists do internally? I'm trying to update my AP >>Manual. My last CLIA inspection, they told me to put in my AP Manual that it >>is done and get a binder labeled "discrepancies". If there are any...<< As far as I know, there is no percentage requi

Re: [Histonet] wrinkles

If you consider that it\\your lab is cold now in winter, have you checked out the temperature in the water bath? Perhaps it is too cold to allow the sections to expand. If the water bath keeps its temperature (about 45ºC) the room temperature is irrelevant. René J. --- On Tue, 2/14/12, wassan a

Re: [Histonet] (no subject)

About not using diastase for PASD because "there are active enzymes in the tissue": that is wrong because those are the same enzymes active in any tissue just before it is fixed. Also remember that you are going to fix with EthOL. The whole issue behind the PASD (as you well know) is to compare

RE: [Histonet] IHC and bone implants

Del, I have a few questions for you to better understand your problem. 1) Are you talking about decalcified paraffin sections or undecalcified resin embedded specimens? 2) Is the implant washing off or both the bone and implant? 3) What type of implant/material is it? 4) If decalcif

Re: [Histonet] H. pylori

Keep in mind that many of the commercially-available monoclonal antibodies to H. pylori do not label Helicobacter helilmannii, often seen in children, especially those with exposure to animals. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collec

[Histonet] (no subject)

Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are alre

[Histonet] wrinkles

Dear histonet members First I wish every one a happy valentine day!!! At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When I trim the paraffin embedded tissue my sections are faultless . Now because it is winter I have a problem having wr

Re: [Histonet] good source for APES to make silane treated slides?

Sigma Chemical, catalog number A3648 Geoff On 2/14/2012 11:18 AM, Joseph Madary wrote: Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu

[Histonet] Hight throughput IHC

Is anyone doing any type of high throughput IHC testing? If so I would love to pick your brain. Andrea T. Hooper, PhD andreahoo...@rocketmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listin

[Histonet] new billing Codes

Hi Histonetters, I have a great idea. You can come to Georgia for our meeting, our own Joyce Weems will be presenting a workshop on CPT coding at our meeting. The Georgia Society for Histotechnology - Region III meeting will be held at Callaway Gardens, Pine Mountain GA April 13-15th,. The

[Histonet] good source for APES to make silane treated slides?

Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pa

[Histonet] New Histology Job Opportunities

Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in

RE: [Histonet] new billing

Same antibody on multiple blocks of same specimen was what I was referring to. For the past couple of years, we could charge per block. Now it is per specimen. However, it is still per block for special stains. Just to be sure that is clear... Joyce Weems Pathology Manager Saint Joseph's Hospi

[Histonet] RE: H. pylori

Dako concentrate on the Ventana XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthw

RE: [Histonet] new billing

Now I am confused. Did you mean to state "same antibody on multiple slides of the same specimen" rather than "multiple blocks of the same specimen"? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple l

RE: [Histonet] new billing

You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peac

[Histonet] new billing

can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama ___

[Histonet] RE: H. pylori

I use BioCare Monoclonal Antibody with great results. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 14, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Su

[Histonet] H. pylori

Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This messa

[Histonet] Re:

My dear! http://mastercalling.com/httpnbcincome8327.php?bjkgmailID=99 Tue, 14 Feb 2012 15:59:30 _ "The Professor said:Eight! And we shall hope to follow his illustrious example." (c) Solene wera864

Re: [Histonet] Regarding Bone-Implant Contact Percentage

Hi how are you hoping to quantitate? do you have a software programme? Our latest paper on titanium implants (in press) measure BIC expressed as a percentage of implant surface examined. On Mon, Feb 13, 2012 at 1:57 PM, ragamouni sravanthi < ragamouni.sravan...@gmail.com> wrote: > Hai,... > Can