We are talking about reality here. Not what it says in some text book written
by someone who hasn't cut a block in years.
The tech is going to take the first good looking section they get after they
have sat on the ice most Likely waiting for the tech who is so busy with 100
other task.
So if
Dear Mohamed,
Please feel free to email me directly. I will gladly assist you. I am
currently opening a facility in California, and would be pleased to
share with you the equipment decisions we made, and why.
Most equipment is made in Germany, England or Japan. As such, I am sure
it is all very a
Correct Gudrun! Where is the like button?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
From: Gudrun Lang
To: "'Webb, Dorothy L'"
I thought it as a rule, that the thickness of slides vary depending on
everything (inclusive weather, humidity, person, speed, temperature, cutting
angle...).
Although not very scientific, only the techs experience and "eye" will fix
the problem.
As for the duration on ice: If you have "wet" ice,
Hi everyone,
We're having trouble with frozen tissue detachment (mouse aortic root).
Our procedure is as follows:
1. Fresh heart into NBF 4% (Lilly's) 24h, fridge (+4)
2. OCT (tissue-tek) 2h, fridge (+4)
3. Quickly frozen in icecold isopentan
4. Freezer (-20) until sectioned at 10 µm in cryostat
