[Histonet] Question about HT examination

2012-03-16 Thread Tuyen Nguyen
I don't have enough credits to renew my HT license before the expired day. I wonder that I could re-take the HT examination before my license is expired ?  Thank you,Mai Truong,Histotechnician Los Angeles California ___ Histonet mailing list Histonet@li

[Histonet] chemical exposure

2012-03-16 Thread Tuyen Nguyen
I am wondering how much the tech exposes to the formalin and xylene during working with recycle equipment? my friend works with recycle equipment, and he still smell  a lot of chemicals( he always wears special carbon filtered mask).Thank you,Mai Truong,Histotechnician, Duarte los Angeles Califo

[Histonet] water bath

2012-03-16 Thread Amber McKenzie
Is there a difference in using DI water vs tap water in the water bath? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: PAS Diastase

2012-03-16 Thread Bob Richmond
Jennifer MacDonald asked: >>At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this?<< If I want an u

[Histonet] MMP2

2012-03-16 Thread Chiriboga, Luis
Hi Any recommendations for a MMP2 antibody for use in FFPE human tissues? Great weekend to all !! Luis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] unsubscribe

2012-03-16 Thread Deb.Krol
Please unsubscribe this address. Deborah Krol Histology Section Head MidMichigan Medical Center 4005 Orchard Dr. Midland, Mi 48670 phone (989) 839-1836 fax (989) 839-3042 deb.k...@midmichigan.org ___ Please note that this email message and any attachments may

Re: [Histonet] Calponin

2012-03-16 Thread Mark Tarango
I think normal breast staining of myoepithial cells is the best control for this antibody. We use BioGenex's antibody (product # MU333-UC) at 1:200 on the Benchmark XT (CC1 mild, 8 mins primary antibody and ultraview DAB detection). Mark On Fri, Mar 16, 2012 at 7:34 AM, Rene J Buesa wrote: >

Re: [Histonet] Medical Exams

2012-03-16 Thread Rene J Buesa
A pre-employment physical is required in many hospitals regardless what you are going to be working with. It essentially refers to your employer health insurance. René J. --- On Fri, 3/16/12, lau...@blufrogpath.com wrote: From: lau...@blufrogpath.com Subject: [Histonet] Medical Exams To: "Hi

[Histonet] Medical Exams

2012-03-16 Thread Laurie
Does anyone know if a pre-employment physical is required if youwill be working with formalin? I have that stipulation in a procedure= that I've had for years, and I don't remember where I got that info. = Laurie

Re: [Histonet] Validation of new equipment

2012-03-16 Thread Rene J Buesa
Your client and immediate supervisor is the pathologists, and s/he is also the one who has to answer directly to your inspecting authority, be it CAP or any other. Therefore, is s/he is satisfied with the way the tissues you process in a new tissue processor turn out, that is sufficient. Any dis

Re: [Histonet] Calponin

2012-03-16 Thread Rene J Buesa
DAKO monoclonal antibody at 1:1000 for 30 minutes, HIER citrate at pH6, colon cancer (+) case as control. René J. --- On Fri, 3/16/12, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] Calponin To: histonet@lists.utsouthwestern.edu Date: Friday, March 16, 2012, 9:14 AM

Re: [Histonet] Staining GAGs in fetal membranes

2012-03-16 Thread Geoff McAuliffe
1. Try an alcoholic fixative like formalin-alcohol-acetic acid 2. Try putting Safranin O in the fixative. Shepard and Mitchell, J. Ultrastruc. Res. 54:451-460, 1976. or same authors used Toluidine Blue O J. Histochem. Cytochem. 24:621-629, 1976. Geoff On 3/15/2012 8:41 PM, Jennifer Scholler wr

[Histonet] Calponin

2012-03-16 Thread Chakib Boussahmain
Hello Histonet Does anyone use Calponin stain? if so can you share the protocol with us? What do you use for epitope retrieval? Many thanks in advance. Chakib Boussahmain Histotechnologist HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern