I've been known to leave a fake spider in an embedder for the morning
person.although it wasn't april fools just for fun it got a great
reaction :)
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Not to put a damper on your fun but something to think about.
I have played my April fools pranks and also been at the other end on many
occasions.
Let us not forget in these high stress times that perceptions vary considerably
and what one person considers as a harmless and amusing prank to them
Dear Histonet Colleagues,
We have a per diem position to fill for a new lab in the Beverly Hills area
of Los Angeles. Looking for an experienced histotech, ASCP certified, to
fill in when our staff is short. We will not be opening for a couple of
months, however I would really like to get a couple
We have a part-time histotech position available and anyone who is
interested, please send me your resume by fax or email. The work days are
Tuesday through Saturday starting at 5 am.
Thank you,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA 92708
Fax: 714-755-2984
___
Fellow Histonet Subscribers,
I have been noticing a LOT of misinformation being passed around this site,
much coming from one respondent in particular. This is very troubling, as
our position in healthcare, and the work we do, is so very critical to
patient care and patient outcomes. I would hop
Tim-
Antigen retrieval is off-line... you need the decloaker or other device.
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
-Original Message-
From: histonet-boun...@l
Joseph:
ALL commercial antibody diluent carriers contain the same ingredients (bovine
albumen, Tween 20 and sodium azide as preservative in pH7 PBS buffer).
Every 100 mL of antibody diluent contains:
Bovine albumen - 1 mL
PBS pH7 buffer - 99 mL
Tween 20 - 50 µL
s
To those with the Biocare intelliPATH system, Is the antigen retrieval part of
the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom
Truscott
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Neutral buffered formalin for 36 hours at room temperature.
René J.
--- On Wed, 3/21/12, LaSalette Charette wrote:
From: LaSalette Charette
Subject: [Histonet] Fixatives for breast
To: "histonet@lists.utsouthwestern.edu"
Date: Wednesday, March 21, 2012, 12:59 PM
What are the best fixatives
Both but especially the place where the diagnosis takes place.
René J.
--- On Wed, 3/21/12, Cynthia Pyse wrote:
From: Cynthia Pyse
Subject: RE: [Histonet] Block Storage-another question
To: "'Rene J Buesa'" , "'Ann Angelo'" ,
histonet@lists.utsouthwestern.edu, "'FeltonNails'"
Date: Wednesday
Regardless where the blocks are generated, that private lab has to follow state
regulations. That will be a legal matter related to any possible law suit after
an alleged misdiagnosis.
If the private lab has no available space, there are companies that can store
blocks and slides for as long the
Hi,
We are searching for a fee for service lab experienced in cryostat sectioning
of animal and human tissues. Any recommendation is appreciated.
Thanks,
Shu-Cheng
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse
Amen!!
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Exactly, we are trying to educate our staff on this currently. It is
important to understand because the regulatory bodies do focus on this
issue.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly
Boyd
I would be very careful when you say you need a CLIA certified tech to do gross
(high complexity testing). You actually need someone who meets the CLIA
requirements to perform High Complexity Testing. You can have a tech who is
certified, but they may not meet the educational requirements. That
I have used the Renaissance Background Reducing Diluent from Biocare Medical.
It works great on human and rodent tissues. Biocare also had a wide selection
of diluents and blocking reagents when used together can significantly reduce
background and enhance staining intensity.
Best Regards,
Da
You dont send your blocks out when you send your slides to be interpreted for
the professional component do you? Ive never seen that done personally.What I
have seen is the lab that created the blocks stored themand just sent slides
out to be read. And if it were me in this position I'd say keep
What are the best fixatives for breast specimens? Also what the most commonly
used fixatives out there?
LaSalette
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Thank you for your input. We always go by the most strict regulating body
which is NY and we save our blocks 20 years. Ann
-Original Message-
From: Cynthia Pyse
To: 'Rene J Buesa' ; 'Ann Angelo' ;
histonet ; 'FeltonNails'
Sent: Wed, Mar 21, 2012 11:25 am
Subject: RE: [Histonet] Bl
I know to hold them for 20 years, but where? At the lab where the blocks were
processed or at the lab where the slides were read? Ann
-Original Message-
From: McMahon, Loralee A
To: Cynthia Pyse ; 'Rene J Buesa' ;
'Ann Angelo' ; histonet ;
'FeltonNails'
Sent: Wed, Mar 21, 2012 11:
I like the DAKO antibody diluent.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2588 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
I guess everyone's idea of 'fun' can be different. A lot of these things sound
like a lot of work to 'clean up'. Funny is different than making a mess to
me--just 1 cent worth :-)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsout
I'm loving it!
Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I'm with you Kara. No harm no foul.
Lee Ann Baldridge
IUSM
Indpls., IN.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Wednesday, March 21, 2012 12:19 PM
To: dstilli...@unipathdx.com; s...@
Nope. a fun work environment breeds better workers.
> From: dstilli...@unipathdx.com
> To: s...@stowers.org; karabo...@hotmail.com; sbree...@nmda.nmsu.edu;
> tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu
> CC: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] April fool
I second that. I hope you are kidding! Hm.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon
Sent: Wednesday, March 21, 2012 9:13 AM
To: 'Kara Lee'; sbree...@nmda.nmsu.edu; tajib...@echd.org;
There are a few I remember from the past:
Telling the pathologists that the processor has been delayed or stopped
running in the alcohols. If you ever want to see them go bonkers - that
one will do it.
Loosening or taking out of bulbs from microscopes - not a very nice thing
to do but I've heard
Please tell me you are kidding about all this stuff!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Wednesday, March 21, 2012 10:43 AM
To: sbree...@nmda.nmsu.edu; tajib...@echd.org;
I would think that if NYS is involved in anyway you have to hold those blocks
for 20 years. When they inspect you do they inspect both labs? Do both labs
hold a NYS permit?
For cetain I would call NYS before you discard anything.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
St
While I'm new at histology, I am a master of pranks.
Here's a list of some fun ones I've done to co-workers over that years...that
haven't gotten me fired or killed yet ;)
1. fill their desk space with packing peanuts
2. If you have the time, post-it note their entire office, office wall, ceil
Hi all, I would like to get a commercially prepared antibody diluent with
carrier proteins. I have been told Dako has a decent one but I am trying to
find a different vendor. I see a few places with diluent but none that have
background reducing substances/carrier protein type thing.
Nick M
What a horrifying idea to put charcoal -or any material that produces dust- on
a microscope.
I like the blank slides idea though.
Van: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] namens Breeden, Sara
[sbree...@nm
I would be very careful when you say you need a CLIA certified tech to do gross
(high complexity testing). You actually need someone who meets the CLIA
requirements to perform High Complexity Testing. You can have a tech who is
certified, but they may not meet the educational requirements. That
I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's
eyepieces. Vaseline on the coffee cup handles (don't all pathologists
consume Mass Quantities of the stuff???). A tray of completely blank
slides, all properly numbered for the day's work?
Feed me some good ones, too. I'm dow
If the pod lab is in NJ and the "reading" lab is in NY, which guide lines do
you follow. NYS requires us to save our blocks for 20 years. Due to storage
issues, I would rather the pod lab store the blocks.
Cindy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:h
Karen writes:
To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana
Benchmark:
I have been trying to stain a nerve (cross and longitudinal section) and it
keeps falling off of the slide after the neurofilament stain. It's been cut
onto a charged slide and has been baked 20 minutes
Rene I think this question is eluding to a Physician owned lab and who is
responsible for storing the blocks not how long they are retained?
From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Wednesday, March 21, 2012 9:20 AM
To: 'Ann Angelo'; histonet@lists.utso
Any ideal for April fool's prank?
Tunde Ajibade BS, HTL(ASCP)QIHC
CONFIDENTIALITY NOTICE: The documents accompanying this email transmission
contain confidential information belonging to the sender that is legally
privileged. This information is intended only for the use of the individual o
Allied Search Partners is currently looking for a qualified applicant for a
Histotechnologist for a 3rd shift Lead position available in a Fort Myers,
FL laboratory.
Position: Histotechnologist
Schedule: 3rd shift/full time permanent position
Summary:
Perform a variety of routine and specia
Our DI tanks are provided and serviced by Seimens. They do the testing.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@no
When you introduce the words "reagent quality" it means that a chemical
manufacturer has done specific tests to assure that the water complies with
such designation. Deionized water is usable for solutions preparations but the
issue resides in the deionizer that has to be tested as to its perfor
In regards to CAP's Lab General Checklist questions regardin= g water
quality:
Is deionized water consid= ered CLRW (Clinical Laboratory Reagent
Water)?
Is water testin= g necessary for a Histology Lab? If so, how do
small labs test f= or resisi
Depending on the state regulations and the laboratory policies,blocks are
stored during different periods of time before being discarded. I used to store
them for 9 years.
René J.
--- On Wed, 3/21/12, Nails, Felton wrote:
From: Nails, Felton
Subject: RE: [Histonet] Block Storage
To: "'Ann An
Use it as a regular mountant and coverslip.
René J.
--- On Wed, 3/21/12, Caitlin Hesketh wrote:
From: Caitlin Hesketh
Subject: [Histonet] Thermo Scientific "Aquamount" procedure?
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 21, 2012, 9:08 AM
I have a bottle of Thermo Scientif
I am looking for a used VCP cassette printer from Leica/Surgipath. My print
head finally went out. Thanks.
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To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana
Benchmark:
I have been trying to stain a nerve (cross and longitudinal section) and
it keeps falling off of the slide after the neurofilament stain. It's
been cut onto a charged slide and has been baked 20 minutes prior to IHC
I have a bottle of Thermo Scientific Aquamount and a path who is insisting
that I start doing AEC chromogen on our immuno slides. We're still hand
staining immunos, although we have a Benchmark Ultra now, the doctors are
dragging their feet on reading the validation slides.
Anyway, how exactly do
We do keep "minimum volumes" of alcohol and xylene in the safety cabinet. But
we can't order so much that some are stored on the floor outside the
cabinet--keep just enough that you use in a reasonable time frame. We get
alcohol in hospital, weekly, so it is not a problem.
Peggy
Peggy Sher
This is a very sticky issue, when I setup inhouse labs I always present the
argument that during inspections the lab that produces a report should have
access to all test material which includes blocks and slides per the CAP
checklist.
Ann trying approaching it from that angle.
-Original M
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