I've used pHH3 to mark proliferative cells and it works pretty well.
Active-caspase-3 and cleaved-parp have worked well for staining apoptotic
cells as well.
-Mehlika
> From: l...@premierlab.com
> To: b427...@aol.com; nmargar...@childrensmemorial.org;
> histonet-requ...@lists.utsouthwestern.
Anyone know if there is or has used a good Anatomical Path LIS that is
compatible or user friendly with Greenway. I really don't know a whole lot
about this stuff , but could use some ideas. Thanks Histojoe
___
Hi
I agree with Jackie, we use Ki-67 all of the time and I have never seen it
stain apoptotic cells, could you possibly be dealing with some background
staining due to the detection system used? I do not know what type of samples
you are staining? We have 4 different Ki-67 antibodies we use depen
Are using any adhesive on the slide or a charged slide? We used to dip slides
in a water/elmer glue solution and allow to dry. Then place a section on it
and it seemed to work...
Sent from my HTC on the Now Network from Sprint!
- Reply message -
From: "Kienitz, Kari"
Date: Thu, Apr 1
Ki67 does not stain apoptotic cells. Why do you have that impression?
Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as
well as necrosis, but not proliferation. I've used Ki67 for proliferation
and Caspase 3 for apoptosis routinely in cancer research for years.
Hi
I'm trying to switch from cacodylate buffer to Hepes in my EM-fixative.
PBS buffer is not an option.
My fixative contains sucrose, CaCl2, 2,5 % formaldehyde and 2,5% GA in
addition to cacodylate (later Hepes). Formaldehyde is "homemade" stock
25 % (no methanol)
After storage for some time
Hi histonetters,
I am looking for the good markers to detect (separately) proliferation and
apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic
bodies as well as proliferated cells; and the tunnel assay shows both apoptotic
body and proliferation.
Any suggestions for th
Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco asks:
>>Does anyone know where to get specimen boards that you can pin specimens to
>>and then submerse in formalin? I ordered them a long time ago and cannot
>>remember where I got them.<<
I've solved this problem several
After cutting, try putting the slide into a coplin jar of formalin. Introduce
to heat for about 30 minutes or so, remove and let air dry before staining.
Kari Kienitz HT, (ASCP)
Histology Laboratory
Portland Gastroenterology
The Oregon Clinic
NE 99th Ave
Portland, OR 97220
503.935.8311
kk
Hello to all in histoland. We have a stubborn toenail that keeps coming off
when we try to do a GMS stain on the ventana machine. Any suggestions on how
to keep the section on the slide during the staining procedure.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONF
Marston Technical from Cincinnati, Ohio has supplies. Their phone is
513-563-8100.
On Wed, Apr 18, 2012 at 3:35 PM, Lyn Stadler wrote:
> Anyone out there using a VIP 3000? I need some replacement gaskets that
> Sakuara no longer manufactures. Anyone know of an alternate vendor or
> supplier th
Our client is a leading clinical laboratory currently seeking a
Histology/IHC Manager, Monday thru Friday, to oversee operation &
administration of several departments. Based in southern California, this
individual will work closely with pathologists & other medical professionals
to effectively me
Yes, I wish for that too.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> CC: tgo...@mt.gov; nko...@chw.org; histonet@lists.utsouthwestern.edu
> From: one_angel_sec...@yahoo.com
> Subject: Re: [Histonet] RE: Unregistered HT testing
> Date: Thu, 19 Apr 2012 13:31:07 -0400
> To: joellewea...@hotmail.com
As usual we all have our own opinion.
High complexity acceding to CLIA is a defined measurement. In other words
things like is the task heat, ph , time dependent, accurate measuring ? Those
examples make a task high complexity.
The above us exactly why grossing is considered high complexity b
Tresa I do see your point. I guess to me setting up IHC and validation takes
some insight and knowledge, but once it is running on a platform, it is pretty
much the same as far as your hands on and gets pretty routine. Those that get
to still do manual IHC get a little more challenge. Since I
I disagree with your assessment of complex staining. IHC staining is like
"cookie-cutter" staining - one does the same steps every single time with a
different (but very similar) set of reagents. The quality of special stains on
the other hand are determined by a unique chemistry - one can get
I have a grossing position open, I have a non certified. Non degreed awesome
tech with 27 years. Anyway I can have her Gran-fathered in by CAP regulations?
I'm pretty sure I know the answer I am hoping to get around this, Cheri
Cheryl A. Miller HT(ASCP)cm
Histology/Cytology Prep Supervisor
Physi
I am wondering if any anatomic pathology labs out there have "leased" a FTE,
histology tech, to a GI practice to run a histology lab in the Endoscopy
clinic? How did you logistically accomplish this? You can contact me offline
for more details. Thank you in advance for your responses.
Jason McGoug
Sounds like the specimens are not fixed well enough!
Josie Britton HT(ASCP)
Cheshire Medical Center
Keene, NH 03431
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Peterson, Dan
Sent: Wednesday, April 18, 2
Do any of you know of companies or individuals that can repair an old Shandon
Hypercenter XP processor? They don't even make parts for it anymore.
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After spending 7 1/2 years as a technical specialist with Ventana, I would also
like to say that putting the control on the bottom of the slide and the patient
at the top is just added insurance that the patient will receive the bulk of
the reagents vs. the control which will help ensure against
I still use batch controls, but I am one of the few left that is not automated.
I do everything by hand. I think you are right though, placing a control
tissue on each slide is the only way to be sure that everything was dispensed
correctly...especially if you are using a Ventana...
Sarah Goe
All,
I place a positive control on each slide, next to the patient tissue for all of
the reasons already mentioned, but we are missing the obvious one.
Many of us use some kind of automated immunostainer where there is no
"gaurantee" that, because the CD3 in position #4 (batch control) wo
At previous institutions I purchased rolls or sheets of cork and that allowed
us to pin and float samples like neck dissections, segments of colon etc I
know Fisher sells the sheets and the roll of cork we purchased from an internet
retailer. Just do a Google or Amazon search.
Making the pa
Hi Karen -
Are yoru eferring to the boards that have the detachable mats? I know they
still make them and the last one I heard of was through MOPEC.
I'm probably aging myself here, but we also used to make paraffin blocks
for large specimens and pin them flat for overnight fixation - it wasn't
p
We used to make our own. We would get a large shallow cardboard box, fill it
with paraffin. Let it cool. Then peel the cardboard away
You can submerse that in formalin. And you can custom cut them to whatever
size you need.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Stro
Hi Eric
Thanks for your input regarding the Citrate buffer pH2.0. Yep this pH came
as a surprise to me too. The data sheet from the company specifies 0.01M
citrate at pH2.0 and a publication which used the same antibody also
indicates a "modified citrate buffer" but doesn't give the exact p. I wil
Does anyone know where to get specimen boards that you can pin specimens to and
then submerse in formalin? I ordered them a long time ago and cannot remember
where I got them.
Thanks,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca
Hi all,
I would like to get some advice from the experts. We are a research lab
currently working primarily with mouse and zebrafish tissues. One of our
researchers has access to tissues from local hospitals which have been
processed and embedded. Having been in research and away from the cl
Hi all,
Just thought I'd share this with y'all. I have just received a booklet
from Leica Microssytems "101 steps to better histology", which i think is
an excellent overview and ready reference for practical histology. It has
great photos of common problems such as sections contaminated with sq
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