Re: [Histonet] Embedding beads

Hi All Thanks for the correction Dr Richmond. It is Hama Beads. We keep ours in used rectangular cover slip boxes, which hold many beads of individual colours in each box. When in use each embedder has their allocated box at the back of the embedding centre, close to the dispensing spout, so th

[Histonet] Re: Embedding beads

I've never seen beads used for identifying individual embedders by marking blocks with the beads. I never heard of Hama Beads (that's how it's spelled), which I think may be called Perler Beads in the US, but I never heard of them either. I think you can get them from Amazon. Bob Richmond Samurai

[Histonet] (no subject)

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[Histonet] Thank you to all who responded

To my Microm HM 500 OM User manual request!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE: Muscle Biopsy Procedure

I have done the same thing with acetone instead of alcohol... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] RE: Muscle Biopsy Procedure

One place I was at didnt get many muscle biopsies so we didnt keep liquid nitrogen and isopentane, which is what I was trained to use initially. Here's what we did when we got a muscle biopsy and it worked fine.   After cutting the biopsy into 3-4 mm portions, we wrapped it in aluminum foil and

RE: [Histonet] Embedding beads

Another plus for barcoding and going paperless. Scanning the cassette logs who embedded the tissue and you can see what type of tissue it was, how many pieces should have been there and if there were any instructions such as on end or on edge. Victor Victor Tobias HT(ASCP) Clinical Application

Re: [Histonet] Embedding beads

I called on a lab in Las Vegas when I had my sales job that used sequins. Well, it was Vegas! Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu

Re: [Histonet] Re: Tolivia, Navarro and Tolivia

I have sympathy for your dilemma! While I have no experience with the procedure in question there have been many, many publications since the 1960's trying to address this issue. Personally, I have never seen one I though was much good/worth the effort so I have just stayed with toluidine blue i

[Histonet] RE: Muscle Biopsy Procedure

Ian, Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010

[Histonet] Re: Tolivia, Navarro and Tolivia

Hi Again Gayle, I'm sorry I was not very clear; I have already downloaded and read the publication, and I am trying the procedure out today. I was wondering about using a couple of substitutions and thought I might ask if anyone has tried this procedure before. I am on a four-week research projec

Re: [Histonet] Embedding beads

We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab. There has to be accountability for every job. Jen Campbell On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie < amber.mcken...@gastrodocs.net> w

[Histonet] Embedding beads

Does this slow the embedder down any having to pick a bead up each time to embed along with the tissue? I've never heard of this procedure before. The labs I've worked in have an embedding log and we initial each case we embed. What do you keep the beads in and are they set in a particular spo

[Histonet] Re: Tolivia, Navarro and Tolivia

My apologies Gayle, I am new at this... >From Histochemistry (1994) 101:51-55, "Polychromatic staining of epoxy semithin sections: a new and simple method," by Tolivia, Navarro and Tolivia. I am wondering if anyone out there has tried this technique. Thank you for your time in responding! --Shan

[Histonet] Tendon Processing

Morning all, I am taking over some work on tendon tissue and would like some advice regarding processing schedules. The tissue is 3-5mm thick cross sections from large tendons and is formalin fixed, paraffin wax embedded. At the moment the sections (although flat and wrinkle free) appear cracke