Shelia
I am sorry that you have received conflicting information on this question.
What we should all have asked you to start with is what did you finally want to
end up with?
We all assumed that you just wanted to stain frozen sections.
If just looking at frozen section then I do agree that Oi
Hello,
Does anyone have experience with reusing the BMDS transponder chips?
Most importantly, are they autoclaveable? If not, I'm looking for suggestions
to sterilize them (and the inplantation device) for reimplantation.
Thanks,
Steve
___
Histonet
Sheila, You can't normally use osmium lipid fixation for paraffin sections
because the lipid you want to see will usually have been dissolved out by the
paraffin processing. You need to either post-fix in osmium (after formalin)
before paraffin processing or use frozen sections. As mentioned, on
I don't pay $1 per slide for the leica slidesso you might want to ask your
salesrep.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.uts
We can buy locally made plus slides for $1.25 per box of 50 and we can
buy imported Leica slides for about $1 per slide.
The Leica slides are very nice indeed, but the locally made ones provide
similar performance.
E. Wayne Johnson
Enruikang AgTech
Beijing.
On 8/24/2012 11:34 PM, Bass, Caroli
We use the Leica Biosystems X-tra slides with a lot of success for Brain and
bone.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwe
Hi Everyone,
I'm trying to buy super frost plus slides (or color frost) for adhering brain
sections. Fisher is very confusing on this, and I don't really remember buying
slides for $1 each. Can someone recommend a source for plus slides that is
cheaper?
Also, I just started using labels for my
Osmium tetroxide solutions will fix your skin on contact, but skin grows
back. Osmium tetroxide fumes will fix your corneas which are irreplaceable.
Osmium tetroxide is dangerous, but it is manageable. First, make sure your
fume hood draws really, really well. Put 100 ml of pH 7.2 phosphate-
Two other arguments against osmium, it is very expensive and is must be
disposed of as hazardous waste.
Geoff
On 8/24/2012 10:50 AM, Sheila Adey wrote:
Thank you so much for this response. :)
Subject: RE: [Histonet] Osmium tetroxide staining for lipids
Date: Fri, 24 Aug 2012 08:42:34 -060
Thank you so much for this response. :)
> Subject: RE: [Histonet] Osmium tetroxide staining for lipids
> Date: Fri, 24 Aug 2012 08:42:34 -0600
> From: billodonn...@catholichealth.net
> To: rjbu...@yahoo.com; sa...@hotmail.ca; histonet@lists.utsouthwestern.edu
>
> I agree w Rene on this one - d
Barry,
I suspect they are wanting to employ this on paraffin sections as Sheila
mentions using it on routine 4 micron sections>
If that is the case, there are published articles using Sudan Black B and Oil
Red O (Histopathology 2002, 41, 75-79) in paraffin sections, and I believe Tim
Morken
I agree w Rene on this one - do not use this stuff if you do not have to - and
in 2012 (or at least in the last 20 years or more) - you do not HAVE to! - Oil
Red O is far safer - Bill
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsou
For this difficult tissue, and for any other for that matter, add to your
regular round water bath at 45ºC, 2 mL of liquid dish washer soap. The soap
will reduce the surface tension of the water and will allow the sections to
extend completely, eliminating any folds.
René J.
__
Osmium tetroxide is one of the most dangerous substances you can use in the
laboratory. Your pathologist probably read some article or found an old photo
of fat "stained" with osmium tetroxide and now wants you to do the same thing.
The problem is that the fat is allowed to react to the fumes of
238 bed acute care hospital with affiliated reference laboratory in the MidWest
is currently looking for a histotechnician or histotechnologist (ASCP
registered or equivalent preferred). Responsibilities will include, but are
not limited to, specimen processing of surgical and cytology specimen
I know that animal tissue is very different than human and we deal only with
human. But we share the same issues here. We have found that floating the
tissue in a room temperature dish of 30% alcohol and then transferring them to
the water bath has helped us.
Interesting to hear what others are
Sheila Hi
I am assuming that you are using frozen sections?
The easiest way is to put a small amount of aqueous osmium tetroxide in the
bottom of a dish that has a tight fitting lid.
Place the slide with section into the dish but not touching the fluid.
Place tightly fitting lid and leave - not su
Try floating your sections on room temperature (in a shallow dish) water then
carefully, on a slide, transfer to the waterbath. Sections will spread better
when they hit the warm water.
Good luck!
Sheila Haas
Laboratory Manager
MicroPath Laboratories, Inc.
From: Jennifer Sipes
To: Histonet
S
Good morning/afternoon/evening Histonetters!
I'm having a problem with my tissue folding. It's tiny folds in mouse tissue
that are too little to see without the use of a microscope. I was wondering if
anyone has any tricks that might eliminate this so I can ensure that I'm
turning out the be
Hi Everyone:
One of my pathologists wants me to look into Osmium Tetroxide for staining
lipids. From what I can gather on the internet, it looks like it is used in
Electron microscopy for fixation and staining.
Is anyone using this procedure for routine 4 micrometer sections?
Thanks
:)
Sheil
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