RE: [Histonet] Re: Cooling paraffin blocks with ice

2012-09-30 Thread joelle weaver
Glad if that is some of the information you were hinting at. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: carl.ho...@kcl.ac.uk > To: histonet@lists.utsouthwestern.edu > Date: Sun, 30 Sep 2012 17:27:49 + > Subject: [Histonet] Re: Cooling paraffin blocks with ice > > Nice one, Joelle. >

[Histonet] Re: Cooling paraffin blocks with ice VS. Freezing Spray

2012-09-30 Thread Hobbs, Carl
I don't like ice-trays. Well, I tried them and.they swelled me blocks! Stick them blocks in the fridge, instead! Sure, cooling them blocks is damn fine to get very thin kidney sections... Diane! The wonderful Chief Tech of Gt Ormond St kids Hospital Where are you??? You know all this stuf

[Histonet] Re: Cooling paraffin blocks with ice

2012-09-30 Thread Hobbs, Carl
Nice one, Joelle. Good points I thank you for that sophisticated paper! Yep, wax is not JUST wax. It indeed has a crystalline, sophisticated structure. Yes, cutting good sections depends upon temp/speed of cutting/angle of knife/additives/effectiveness of processing. May be more variables .

Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

2012-09-30 Thread Akemi Allison
Well said Rene!  In my 40 plus years as a supervisor, manager, technical support manager, and consultant; I have come across a broad spectrum of experienced and inexperienced histologists.  Their knowledge base may be limited to their own experiences, or lack of it.  A good supervisor or ma

[Histonet] freeze spray vs ic for microtomy There is a petient on the other side of the coverslip

2012-09-30 Thread madary
&nb= sp;Perhaps you can tell your mgr that you respect her style and method, and= show her that your style and method is just as effective. When Lee Luna wa= s my manager he said time after time "techniiques are as varied as technici= ans". If at the end of the day you are

Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

2012-09-30 Thread Rene J Buesa
Jenny: There is a saying that "life is too short to drink cheap wine". In the same way life is too short to be frustrated daily working at a place where work is like a daily uphill battle. For what you describe you know your trade. Start looking for another place although do not expect that your

Re: [Histonet] Negative Controls in IHC

2012-09-30 Thread Rene J Buesa
Negative controls for IHC has been discussed before many times but it keeps pupping up every noew and then. This is my take on it: 1- if CAP requires them, so then  do it. You do not want to have a negative issue in your inspection 2- it is always good to have them for quality control of your pro