I think that is a good approach but I do not think that an inclusion in GMA
plastic is required.
A well infiltrated FFPE tissue can be sectioned at 1-2 µm
René J.
From: Patsy Ruegg
To: 'Histonet'
Sent: Monday, November 12, 2012 4:16 PM
Subject: [Histonet] rad
Does anyone have any ideas on how we might demonstrate radiation injury to
peripheral nerves near the prostate (in a canine model). we would like to
evaluate demyelinating changes and it has been suggested we might do this by
embedding the tissue in GMA plastic and cutting 2 micron sections and do
ProPath, a progressive, CAP accredited, high volume, pathology practice,
located in Dallas, Texas, is seeking a Histotechnologist. In this position
you will be responsible for embedding tissue specimens, microtomy of
paraffin-embedded tissue, operation of automated stainer and coverslipper,
equipm
I think that instead trying to guess that "mysterious" step, you should contact
the company that developed the kit and ask them.
Maybe their reagents require that.
Never assume!
René J.
From: "Parker, Helayne"
To: "'histonet@lists.utsouthwestern.edu'"
Sent: Mo
Hi All,
Is there any specific reason why one iron stain kit I have recommends that
you air-dry the bone marrow and blood smears after the Fe stain is completed
and then coverslip them while other kits don't mention that at all. I have
always stained my smears then dehydrate, clear and cover
Anybody using EKI Safe-Clear (xlene substitute)? I just would like to
know what mounting media you are using with it. I like the stuff but,
just need to find a mounting media that goes with it :)
Nancy Heath, HT (ASCP)
Neuropathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology
Hi all,
I am posting this for a friend. If interested, please see the contact e-mail
listed below. Good luck!
Supervisor: Anatomic Pathology & Central Processing
Requirements: BS degree, 3 years experience as a Cytologist or Histologist,
and 2 years of management experience.
Since its f
A detection that works for rabbit should be OK. For the Bond you should be
able to use the Novolink polymer (on human) samples with great results. I
use it with the Biocare Nemesis and get excellent staining. You may need to
adjust your protocols when you go from one automated platform to anothe
I should specify and I apologize for not putting this detail in the original
email. I am looking for information pertaining to mouse hosted human xenograft
tissues. We are not having problems when it is species specific within a host
( e.g. rabbit mAB on human or rabbit mAB on mouse. )
Thank
Hello everyone.
I was wondering if anyone has any suggestions for rabbit mAB secondary kits?
We are currently using Biocare and Dako reagents and have recently started
switching many of our optimization projects from the Dako Autostainer platform
to the Bond III platform with research detect
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