Hmmm...I have raised this issue beforeno answer.
All answers to datepure conjecture!
Lol...if you meant "Holly".rather than "Holy" or even "Holey"...nice one,
Batwoman!
Caped Crusader.
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The reference for the "Nuclear bubbling" article is:
Journal of Histotechnology, 1990; 13(2):135-6
René J.
From: Patsy Ruegg
To: gu.l...@gmx.at
Cc: histonet@lists.utsouthwestern.edu
Sent: Sunday, January 6, 2013 12:34 PM
Subject: RE: [Histonet] holly nuclei bat man
Yes this all makes good sens
Yes this all makes good sense and explains why I may not be able to have
control of this because I do not always do the tissue fixation and
processing myself. That first step of adequately fixing (cross linking the
proteins) to protect them from tissue processing and AR are so
important. The
Filter your Giemsa solution before using it or better yet, get a newer staining
solution
René J.
From: Shahram Sabeti
To: "histonet@lists.utsouthwestern.edu"
Sent: Sunday, January 6, 2013 10:07 AM
Subject: [Histonet] a problem with giemsa stain for h.pylori
hello every body,
on giema stai
According with some tests I made and an article published in the JOH, the so
called "nuclear holes" occur when a section with some remaining amount of water
underneath is dried in an oven at 60ºC or more.
The "explanation" is that the heated underlying water "dissolves" nuclear
material and henc
hello every body,
on giema staining of gastric tissue slides for h.pylori,we have encounterd
a major problem for a while.the problem is deposition of large flakes on the
mucosal surface and we do not know why?
do you know how to get rid of this problem.
thank you in advance for your kind help
I have a quiet semi-scientific explanation. Formalin-fixation stabilizes
proteins via crosslinking. AR "solves" methylol-adducts due to formaldehyde
and perhaps also some crosslinks. Nuclei also have a protein-scaffold,
filled with nucleinacids. It seems like a "ladder" or "hole in tights". It
seem
