Good point by Teri Johnson.
I don't decalcify, thus my results may well be compromised by this
procedureand, sure, the bone is disrupted.
I am only interested in CNS/PNS so, I don't worry about bone/cartilage
disruption.
I fix in std 10% NBF.
Imho, it is better to "overfix" rather than to wor
Hi Histonetters !
Can anyone suggest how to prevent adult mouse skin sections from falling
off during in situ hybridization? How to make sections adhere better to
slides? I am using poly-lysine coated slides for 5-10 micron thick skin
sections. The tissue is fixed in 4% PFA and embedded in paraffi
