We have had issues like this in the past on occasion and it usually traces back
to a slide hydrophobicity problem, and completely random. It also seem to be
affected by certain tissues more than others when it occurred, namely breasts.
Sheila
-Original Message-
From: histonet-boun...@l
From: Artim, Kimberly [kimberly.ar...@sluhn.org]
Sent: Friday, August 09, 2013 2:41 PM
To: Davis, Cassie
Subject: RE: Rusty Histo stainer
Is anyone using bleach to clean the stainer? Bleach corrodes the stainless
steel.
Kimberly R. Artim AST, HT (ASCP)
St Luke's Specialty Lab
77 S. Commerce Wa
I've only seen it happen with regressive stains. The acid seems to get in the
air circulating in the stainer.
Cassandra Davis
cda...@che-east.org
302-575-8095
--
Date: Fri, 9 Aug 2013 15:00:13 +0400
From: Dmitry Kislov
Subject: [Histonet] Rusty histostainer, anyone
Hi all,
Looking for pros and cons on immunostainers for a small volume laboratory.
Thanks in advance,
Valerie
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Hi,
Leica 1850s are very reliable, 1860 is basically the same unit with very
precise electronic specimen feed.
UV lamp's lifetime is about 8k hours, and every switch on cycle adds
another hour to the counter. New lamp will cost you a few hundred euro,
though. Any operator can replace the lamp, no
Hi Cindy, I don't use the Dako system or any of the antibodies you mention, but
this kind of sounds like an antibody diluent problem, where either what you
dissolve the antibody in doesn't spread out evenly on the slide or the antibody
doesn't dissolve well in the diluents you use. In an automat
Toni, we use the Thermo NX70 Cryostar and really like it. It is has a
comfortable ergonomic shape, touch screen controls, a debris vacuum and an
elevator for different sized people. The refrigeration system is excellent and
cools the chuck and knife separately. The decon is easy and uses a solut
Has anybody had issues with the scent of the slides coming off the Symphony
Auto Stainer. Our pathologists are complaining of an odor from the slides , and
we just recently started using the stainer.
Sent from my iPhone
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Happy Friday Histonetters
I have a mystery. First a little background. We stain our immuno’s slides on a
Dako 48 with the PT modules for pretreatment. We have not changed any protocols
or antibodies since we optimized and validated the instruments. The majority of
our antibodies are Dako but
Hi All,
Thank you all for your suggestions. I finally figure out our issue. I wasn't
putting down a cover slip. That was it. I didn't realize the hematoxylin
wouldn't appear brilliant blue without one. I went back and added a cover slip
to all of the slides and it looks beautiful.
Thanks for
Has anybody had issues with the scent of the slides coming off the Symphony
auto stainer? Our pathologists are complaining of an odor from the slides, and
we just recently started using the Symphony.
Eileen Teran
Sent from my iPad
On Aug 7, 2013, at 5:18 PM, "Jay Lundgren" wrote:
> Sanjeet,
Happy Friday everyone!
I'm looking for some feedback on cryostat preferences. We are replacing one of
ours now and will be evaluating a few before making a final decision. The 2
I've looked into so far are the Leica 1860 and the HM525 by Thermo Scientific.
I'm interested in the UV feature that m
Hello,
Did anyone ever seen any histostainer got rusty? We have a strange issue
with one of ours - some parts which should be made of stainless steel
suddenly started to get rusty... I don't want to put a manufacturer's name
in here as we are trying to negotiate our way out of this issue now. We'r
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