May I ask please, has anyone heard how many Dako Omnis systems are out there?
And where?
Who are these brave early adaptors?
Thanks,
Michael Farmer
McEvoy & Farmer Pathology
NY, NY
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For registry quality slides, rotate everything except paraffin every 200
blocks (basically every day unless you do tiny runs). Rotate paraffin
weekly, sooner if it starts smelling strongly of clearant. But then, I'm
conservative, and I was trained where reagents were free.
In the past we had a fewer number of processors and fewer variables so we based
changing our chemicals on the day of the week. Now since we have more
machines for better workflow purposes we believe changing should be based on
number of cassettes run through each machine. Of course then we
Hi Patrick,
It sounds as though you have a lot going on. It's important to gauge whether
your frozen sections are moth-eaten due to trimming/facing the tissue too
aggressively and tearing chunks of tissue out, if it's freezing artifact, or if
your sample is too cold and you are getting chatter
Formaldehyd binds as methylol-group to basic functional groups on proteins.
The longer the fixation the longer the possibility to bind. The same basic
groups bind acid dyes like eosin.
On the other side leads acidic formalin to degradation of DNA. This can lead
to lower affinity of hematoxylin.
So
Hello fellow Techs,
Has anyone noticed a change in the Henry's Wonder Wax,
Enhanced? (Used for embedding, not infiltrating.) We have noticed that it is a
lot "stickier" when we are sectioning. (To our picks, forceps, and fingers.) We
have checked our temperatures, and all is wel
