Hello,
I have a Full Time/Permanent Pathologists Assistant position available in
the Omaha, NE area. This position requires ASCP certification as a
Pathologist Assistant. I am ideally looking for someone for a long
term/permanent position however, a temp to perm option may be available.
Relocation
"Hartford Hospital", Hartford, CT
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.car.
I am putting together a list of facilities that have discontinued the use of
negative reagent controls for IHC. After the CAP revised the requirement
related to negative controls when polymer-based detection systems are used, we
decided to investigate whether discontinuing the negative controls
Another solution, and one we did almost exclusively for murine T and B cell
IHC was use biotinylated Rat anti mouse CD4 and CD8. The isotype can be
purchased biotinylated from BD Bioscience/Invitrogen or you can buy Jacksons
Rat IgG-biotin.
This totally eliminates a secondary antibody hence
Thank you to those that sent me suggestion on my CD4 problem. I have recently
discovered the problem.
The tumors are mouse tumors but they are injected in-vivo with a test antibody
that is Rat anti-mouse, specifically Isotype (Rat IgG2a). Hence this is why my
secondary Rabbit anti-rat stained so
Dear Orla,
Post fixation, we have stored our bone specimens in 1x PBS while having them
sent out for MicroCT analysis. We have also stored them in 1x PBS at 4°C post
fixation when necessary until further processing without having adverse affects
on our staining. However, we chose conventional
Just curious-what do people consider the time frame for "long term" ?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barry
Rittman
Sent: Monday, December 09, 2013 7:50 AM
To: Orla M Gallagher
Cc: histonet@l
hi
I would recommend storage for long term in 70% ethanol. To prevent drying
out we used glycerin in the ethanol, about 20% of the volume.
Barry
On Mon, Dec 9, 2013 at 7:44 AM, Orla M Gallagher <
o.m.gallag...@sheffield.ac.uk> wrote:
> Thanks to everyone for your comments.
>
> I may not have b
Thanks to everyone for your comments.
I may not have been clear in my question - our researchers don't wish to
decalcify these formalin-fixed bones yet, but rather to store them for more
than a couple of weeks, in case they need to carry out MicroCT followed by
histology later. I'm aware that the
