[Histonet] How to do validation of PLA2R antibody on serum

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Re: [Histonet] Buffer pH for IHC

pH meter; Corning. On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert wrote: > What does everyone use to test the pH of their buffers for IHC? Are pH > strips adequate or do you have to have an actual pH meter? If you use a > meter, any suggestions on a make and model? > > Laurie Colbert, HT (ASC

[Histonet] Buffer pH for IHC

What does everyone use to test the pH of their buffers for IHC? Are pH strips adequate or do you have to have an actual pH meter? If you use a meter, any suggestions on a make and model? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-

[Histonet] lyophilizing company

Histonetters: My lab is needing to lyophilize some skin tissue. Is there a company that offers such services? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Retaining unstained glass slides

Follow the guidelines of your accrediting agency. CAP = 10 years minimum ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than local, st

[Histonet] Retaining unstained glass slides

How long due most labs retain glass slides? Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.com | www.uro.com Disclaimer: The email and files transmit

[Histonet] RE: Zebra-fish embryo histology

Patty, Our lab has processed zebra fish embryos for paraffin sectioning. The embryos were fixed overnight in 4% paraformaldehyde and processed on a biopsy program without any heat. The key is to pop the swim bladders of the fish so that they will lay flat during embedding. We put multiple pi

Re: [Histonet] zebrafish embryos histology

Hi Patty, You sort of piqued my curiosity of what is going on in the zebra fish world and found this youtube, 6 minute film  http://www.youtube.com/watch?v=kZDwo20hl1E&feature=youtu.be About what is going on at Welcome Trust Research on zebra fish but maybe you know all this already.  Anyway,

Re: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

Thank you everyone! You've all been so helpful regarding the mammaglobin. Ventana has called me and I think we got things figured out (at least tenatively) and the wheels are in motion. If it still doesn't work, our rep will come out and see us. Thanks again for your input! Great ideas abound!! O

[Histonet] Re: zebrafish embryos histology

Hi Patty, We did some of these in paraffin in my other life in Kansas City. Essentially for these we processed them in paraffin, and would embed them in Ls, letting gravity do what it does best. We would line them up side by side, as perpendicular to the edge as we could. We had a stereomicrosc

Re: [Histonet] zebrafish embryos histology

Patty, did some zebrafish work years ago, either pure histology or sections after we did whole mount ISH on the embryo's.  As per Jack Ratliff's post, paraffin I found was really tough for orientation and for getting enough sections of such small embryo's.  But would use, as Jack suggested, JB-

RE: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

Hi Abby, Since the concentration is about a third of the original, I would try programming the XT to run the primary for 2 hrs instead of 32 min. You may also need to up the secondary time if that isn't sufficient. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] zebrafish embryos histology

Patty, I have personally never performed histology on zebra fish embryo's, but if I was to test it out on my own I might try using the JB4 Plus GMA kit. It just seems to me that paraffin might cause too much shrinkage and maybe using an MMA protocol might be too harsh on the tissue. Besides, giv

Re: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

Diagnostic Biosystems has Rabbit Monos Website below Mike Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com joelle weaver wrote: >also have IVD mammoglobin, clone 31A5 rabbi

RE: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

also have IVD mammoglobin, clone 31A5 rabbit monoclonal, works really well for me at 1:75- if you need/prefer IVD from cancer diagnostics, the other I posted is ASR. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mrs.abby.map...@gmail.com > Date: Wed, 22 Jan 2014 08:11:11 -0600 > To: histon

RE: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

don't use Ventana, but I use the Abcam concentrate at 1:100, citrate buffer- HIER and it works great. Usually cell marque AB are good, but had good luck with this one, maybe give it a try? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mrs.abby.map...@gmail.com > Date: Wed, 22 Jan 2014 08:1

[Histonet] zebrafish embryos histology

Can anyone give me a method for zebra-fish embryo histology? The papers I've read show photos, but no description of histology in M & M. I need to put several embryos in each block, and get the orientation correct, and put multiple sections on each slide, in hopes of getting one or two that ar

RE: [Histonet] Histogel

Thank you, John! For some strange reason, the URL in your message got garbled/multiplied, but I just took this part: http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf and that worked. The enrobing I do is to trap debris on the surface of a coral skeleton at the interface between "a

[Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

Fellow histotechs, We've been running mammoglobin testing on our Ventana Benchmark XT. However, Cell Marque (our supplier of mammoglobin antibody) has changed the concentration. Our old concentration was 0.12 mg/mL, the new concentration is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal

[Histonet] RE: Thermo Scientific Embedding Centre Histostar

We had one screen replaced early on but no problems since. However, we've had to replace the plastic lids several times and the parts are always on back order. The one that covers the molds breaks right off. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:hist

[Histonet] Sakura DRS 601 basket hook and slide basket

Hello Let me first thank the list for the nice help I have always found here. (for exemple with Perkins Biomedical Services and autostainer bottles) I am looking now for spares for Sakura DRS™ 601. 4368 : Basket Hook (3 of them if possible) 4768 : 20-Slide Basket (at least 3 to 6 of them) Best