[Histonet] Corpora amylacea are destroyed after antigen retrieval?

Has anyone observed corpora amylacea on brain tissue being destroyed after antigen retrieval (for example microwaving in citrate buffer)? We are experiencing this problem, as we are interested to study these structures, and we are not sure if it is a common artifact when preparing slides for IHC

[Histonet] Lens vs sponges

We use sponges pretty regularly in our lab, though have had trouble with them sticking to particularly small and fragile samples. We have switched to using Telfa for the more fragile/delicate samples (works well, although the Telfa is sometimes difficult to cut down to the right size for the casset

[Histonet] re: Neutralization of AZF

Hi Jim, Do you know what exactly you are neutralizing? Does AZF stand for alcoholic Zinc Formalin? One of the best sources for information regarding zinc formalin products is Anatech, Ltd - www.anatechltdusa.com Call them and ask about their technique (and addition

[Histonet] Moh's Job

Hello everyone in the Histoland I am posting a job for a friend; they are looking for a fulltime Mohs' tech at a local dermatology practice in the Houston Texas Medical Center. Please contact Ms. Stephanie Huff at 281-799-0521. Thomas Thomas Huynh HT(ASCP) Dept. of Pathology Histology/ Bone

[Histonet] HISTOPALOOZA APRIL 25 - 27, 2014

Here's another teaser for the upcoming Histopalooza being held at The Lodge and Spa at Callaway Gardens. We're excited to have Wanda Jones, 2013 NSH HT of the year, conducting a workshop. This workshop is titled " Becoming a Better Person in the Workplace and in Life". This workshop is for e

[Histonet] Neutralization of AZF

It's been awhile but we have a procedure listed for neutralization of AZF fixative. One of the ingredients is listed as simply sodium carbonate. Does anyone know if this reagent is correct or should it be sodium bicarbonate? We are thinking it should be sodium anhydrous carbonate but can't

[Histonet] RE: Immunocal

I had good luck with Chelator Cal from BBC Biochemical. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@

[Histonet] 2014 Tri-State Histology Symposium

Registration is open for the Minnesota, Wisconsin and Iowa 2014 Tri-State Spring Histology Symposium that will be held at the DoubleTree Hotel Rochester, Minnesota April 30-May 2. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 30th and conclude

[Histonet] BBB permeability marker

Hello - does anyone have a recommendation for a marker of blood brain barrier disruption in fresh frozen cryostat sectioned tissue? Can I postfix and immunostain for albumin extravasation, or does albumin easily diffuse away from the vessels post-mortem? The tissue was flash frozen in isopent

[Histonet] RE: End time for fixation of breast tissue

Thanks all Glenn -Original Message- From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] Sent: Monday, February 17, 2014 6:54 AM To: 'Morken, Timothy'; Glenn Hauck Cc: Histonet Subject: RE: End time for fixation of breast tissue We have the nurses put the time the breast is pl

[Histonet] Does anyone use grossing canned messages?

Hello All, We are currently exploring changing our LIS, and one we are looking at relies heavily on the use of canned messages for grossing. We have a few canned messages that we currently use, but nothing like what we are seeing with this new system. Does anyone out there use these, and

[Histonet] New York State Histotechnological Society Educational Scholarship

Good morning! All members of New York State Histotechnological Society have the opportunity to apply for corporate educational scholarships, currently there are several available. These scholarships can be used to defray educational expenses, attend professional meetings or buy educational materia