[Histonet] HT Positions in Greenville, SC

2014-03-19 Thread JERRY SANTIAGO
Hello Histonetters, I have been contacted with a site that is looking to fill several HT positions at their Grenville, SC lab. If interested, please contact me and I will give you the contact information. No recruiters please! Jerry Santiago Email: sant...@bellsouth.net

[Histonet] re Electron microscope choices ?

2014-03-19 Thread Steven Weston
Hi all, I'm still looking for ideas about the best types of TEM around at the moment. If you have recently purchased one or have been a regular user of a late model digital TEM please share your experience with me. regards Steve Weston Lab Manager Breathe-Well Centre of Research Excellence for Chr

[Histonet] RE: Slide Consults

2014-03-19 Thread Mitchell Jean A
Amber: I find this EXTREMELY irritating when an outside institution either writes their accession number on the label or puts another label over the top of my hospitals labels. It is not rude to ask other hospitals to not do this; that doesn't mean they won't do it. I will forward you my slid

[Histonet] RE: Slide Consults

2014-03-19 Thread Bitting, Angela K.
I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19,

[Histonet] Slide Consults

2014-03-19 Thread Amber McKenzie
Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand wr

[Histonet] Question on Fluorochromes for Histomorphometry

2014-03-19 Thread Herrick, James L. (Jim)
Dear Histonet Colleagues, I hope everyone is doing well-- We are going to be using a goat mandible model (embedded in MMA) for a project and will need to administer tetracycline and/or calcein (others if suggested) for histomorphometric analysis. Would anyone be kind enough to share their know

[Histonet] AUTO: Bruce Palmatier is out of the office (returning 03/21/2014)

2014-03-19 Thread Bruce_Palmatier
I am out of the office until 03/21/2014. I will be out of the office from March 18th until March 21st. I will be checking emails periodically and will attempt to reply to your message within 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmat...@vwr.com mob

[Histonet] Re: Processing frozen brain samples

2014-03-19 Thread Teri Johnson
Hi Valerie, Why would you need to remove the sucrose? I would place the frozen blocks in room temperature fixative or buffer with one or two changes, especially if you have OCT that needs also to thaw. Why fixative? You may want to consider adding additional fixation if the tissue was minimall

[Histonet] Re: Histonet Digest, Vol 124, Issue 20

2014-03-19 Thread Mark Elliott
We have one tech who does all of the steps in EM processing, staining etc., but we are a research lab. Mark Elliott Centre for Heart Lung Innovation Vancouver, BC Date: Wed, 19 Mar 2014 14:25:15 + From: "Gauch, Vicki" Subject: [Histonet] Question for Labs performing Electron Microscop

Re: [Histonet] Question for Labs performing Electron Microscopy

2014-03-19 Thread kgrobert
It's the same for me here. But then again, we're pure research. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 > We used to do TE

Re: [Histonet] Question for Labs performing Electron Microscopy

2014-03-19 Thread Rene J Buesa
We used to do TEM at our lab. The work volume was not high and one of histotechnologists was trained to do ALL tasks (fixation → printing the photos of the areas selected by the pathologist). When not doing TEM tasks, he took care of any of any of all HTL tasks for which he was also trained. Ren

[Histonet] Question for Labs performing Electron Microscopy

2014-03-19 Thread Gauch, Vicki
Hi everyone, I have been asked to post the following question regarding EM... If you are providing EM services in your laboratory, who is performing the EM, what is their job title and what are their job duties ( do they process, embed, cut,stain,etc. or just certain portions ). Any information w

[Histonet] Histology Laboratory Staffing

2014-03-19 Thread Pam Barker
Hi Histonetters!! I hope everyone is having a great day. I wanted to put up a quick post because I have been talking to some managers who have told me they have been having trouble finding "the right techs" for the positions they have open in their labs. I know it is tricky at best to find that p

RE: [Histonet] Thrombomodulin positive controls

2014-03-19 Thread Curt
We have plenty, we just use placenta, I can send you a block or two if still needed. What's everyone else using? Curt -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of S hay Sent: Tuesday, March 18, 2014

[Histonet] Processing frozen brain samples

2014-03-19 Thread Murphy, Valerie
Hi, I am new to Histonet. Here is my question. We have brain samples that were fixed in 4% paraformaldehyde then placed in 30% sucrose (cryoprotectant) prior to freezing on dry ice. These samples have been stored at -80 for a couple of years. We want to thaw these samples then process them to