[Histonet] Staining FFPE with biotinylated SNA - how to block?

2014-04-15 Thread M.O.
Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the dilu

RE: [Histonet] RE: Retirement

2014-04-15 Thread joelle weaver
Yes we all appreciate knowledgable, helpful & honest vendors. We all depend on each other. Never had the pleasure to meet you, but wish you many wonderful retirement years! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lbla...@digestivespecialists.com > To: wvantilb...@general-data.com; histo

[Histonet] RE: Retirement

2014-04-15 Thread Blazek, Linda
Congratulations on your retirement! May you enjoy your time and remember you're supposed to take a picture of the sunrise on your first day of retirement! And personally I don't think vendors are frowned upon. They are full of wise info. What's frowned upon is blatant advertising. Even the

RE: [Histonet] Retirement

2014-04-15 Thread Michael Mihalik
Walt we wish you the best. It was great working with you. We'll miss you. You were one of the few who really knew their 'stuff' and who was honest and a pleasure to be around. ..hard qualities to find. Good luck Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.

[Histonet] Retirement

2014-04-15 Thread VanTilburg, Walt
I know it is frowned upon for vendors to write to Histonet, but I hope you will indulge me as I have no easy way to say good bye to some great people. I am retiring next Tuesday the 22nd and for the past 11 year I have met many of you in histo land and have enjoyed the help you all have given m

[Histonet] RE: Waste Flammable Info Please

2014-04-15 Thread Teri Johnson
To clarify, we had to account for new solvent once it was put into waste stream, not what we purchased. Also had to account for Still Bottoms that was manifested out from the recycler. In pounds. Not gallons. Don't ask me to clarify further, because I don't understand it well enough to do so. N

[Histonet] RE: Waste Flammable Info Please

2014-04-15 Thread Walter Benton
I agree. We have to complete an EPA form for waste generated and hauled away, not purchased. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrol

[Histonet] RE: Waste Flammable Info Please

2014-04-15 Thread Elizabeth Chlipala
Teri That's interesting since we need to fill out a form every year that tabulates the waste generated and not what we purchase. I thought it was based upon the amount of waste generated. That was not our experience. The state came and audited us once we started disposing of waste at a rat

[Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 GEORGIA SOCIETY FOR HISTOTECHNOLOGY

2014-04-15 Thread Zimmerman, Billie
Just a reminder to register by today, if possible. If you are awaiting funds please put that on your registration form. For the last minute people (aka procrastinators) who are filing their taxes today...you can still reserve a cottage/cabin at the Southern Pine Cottages. Call Kit Reames-McCl

[Histonet] Waste Flammable Info Please

2014-04-15 Thread Teri Johnson
Hi Dennis, I don't think that recycling will fix your EPA license issue. They care about how much you GENERATE through use, not whether it is actually being disposed of or even recycled. We had an issue with the EPA regarding our xylene generation at another facility I used to work in. When I t

Re: [Histonet] Semen analysis training

2014-04-15 Thread Jennifer MacDonald
I would contact any clinical lab in your area. When I worked in the clinical lab we did semen analysis in Hematology. Jennifer From: Chris Duffett To: Cc: "histonet@lists.utsouthwestern.edu" Date: 04/15/2014 10:18 AM Subject:[Histonet] Semen analysis training Sent by:

RE: [Histonet] IHC imaging systems

2014-04-15 Thread Elizabeth Chlipala
Paula There are many companies that have software that will do this for you. I would check the DPA website - www.digitalpathologyassociation.org. The decision you will make is going to be dependent on software capabilities, cost, if you need it to be 510K cleared, etc. Here is a link to the

[Histonet] RE: Waste Flammable Info Please

2014-04-15 Thread Elizabeth Chlipala
Dennis First of all we are in Colorado. If you dispose of more than 650 gallons a year in waste you are considered a small quantity generator, that amounts to one 55 gallon drum a month. Once you become a SQG this requires being registered with the EPA, additional safety training, every othe

[Histonet] Semen analysis training

2014-04-15 Thread Chris Duffett
Looking for a location in the New York City or northern New Jersey area that might be able to help with training 1or2 techs to so semen analysis for motility and morphology. We know of the Cleveland clinic program but sea a bit too far to send staff. Any insight will be helpful Sent from my iPho

[Histonet] Cost of Tissue Processor

2014-04-15 Thread Adesupo, Adesuyi (Banjo)
Hi, How are you guys doing? I hope you are all doing great. Please we are in the market for tissue processor and I will appreciate it, if you guys could give me an idea of how much it cost. We are going to consider all the brands. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC,

[Histonet] Leica,Sakura Microtome Rebuilding

2014-04-15 Thread Klaus Dern
In reference to Ms. Weems, Joyce K. posting of Dec.17.2013 ( Microtome Service ). I would like to add that this information is specifically about retrofitting the advance mechanism on the following Microtomes which are not supported by the manufacturer anymore with an adjustment feature to ellimina

[Histonet] IHC imaging systems

2014-04-15 Thread Paula Sicurello
Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buy

[Histonet] Waste Flammable Info Please

2014-04-15 Thread Dennis Hahn
I have an inquiry specifically for Texas and/or Children's hospitals: What is your current volume of waste flammables and how do you handle it? Are you recycling, or is it removed by an outside vendor? A combination of the two? Suggestions on recycling instrumentation? Any drawbacks? We current

[Histonet] RE: ANP08216 Formaldehyde and xylene testing

2014-04-15 Thread Walter Benton
Annually for that as well Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthca

Re: [Histonet] ANP08216 Formaldehyde and xylene testing

2014-04-15 Thread Chris Duffett
Same annually Sent from my iPhone Chris Duffett Manager, Laboratory Development Pathline/Emerge 845-709-4246 > On Apr 15, 2014, at 12:12 PM, "Piche, Jessica" wrote: > > Thanks for all the responses so quickly. I'd also like to know the frequency > of formaldehyde testing as well? Thanks to th

[Histonet] RE: ANP08216 Formaldehyde and xylene testing

2014-04-15 Thread Vanessa Perez
We test annually for xylene, formaldehyde, and isopropyl Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche,

[Histonet] ANP08216 Formaldehyde and xylene testing

2014-04-15 Thread Piche, Jessica
Thanks for all the responses so quickly. I'd also like to know the frequency of formaldehyde testing as well? Thanks to those who included that as well. Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged.

[Histonet] RE: Xylene testing ANP 08216

2014-04-15 Thread Walter Benton
Annually Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ___

[Histonet] RE: Xylene testing ANP 08216

2014-04-15 Thread Hannen, Valerie
We test every six months. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lis

[Histonet] RE: Xylene testing ANP 08216

2014-04-15 Thread Elizabeth Chlipala
We test every year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premi

[Histonet] Xylene testing ANP 08216

2014-04-15 Thread Piche, Jessica
Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDEN

[Histonet] rare testing

2014-04-15 Thread Houston, Ronald
Looking for a lab(s) that can perform any or all of the following: IHC for nuclear estradiol Beta R, MYC-N, VDR (vitamin D receptor), PDGFR, EGFR, VEGFR, IL-4R Gene expression testing: MET, IGF, BAX (to determine sensitivity to Adriamycin and doxorubicin) and p53 Thanks Ronnie Ronnie Housto