[Histonet] Job availability

2014-08-27 Thread Nalesnik, Michael
Hello Histonetters! I have subscribed to this message board for awhile and would first like to thank you for all of the helpful suggestions I have picked up just by reading the posts. I am writing because we have an opening for a Histology Supervisor in our laboratory. This is at the University

[Histonet] Paper of possible (insect) histology interest, link inside

2014-08-27 Thread Jorge A. Santiago-Blay
http://blaypublishers.com/2014/07/18/leb-21-2014/ If interested, click on 4. LEB 2(1) Ozyurt et al. Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/indiv

[Histonet] RE: Slide remounting

2014-08-27 Thread James Watson
With slides this old you can usually spray the back of the slide with freeze spray and the coverslip will pop off. Then soak in xylene and once any remaining permount is gone, re-coverslip. We did this routinely at AFIP, in the old days. James Watson HT  ASCP GNF  Genomics Institute of the N

Re: [Histonet] Slide remounting

2014-08-27 Thread Bader Siddiki
Hello Soak slides in xylene or equivalent solvent for sometimes till the coverslip is loose, carefully remove the coverslip; you can remount the slides with Permount. You can dissolve the Permount in organic solvents, but the glue will be dissolved and you have to remount the slide. Bader On Wed

RE: [Histonet] protein A problem with Staph aureus immunostaining

2014-08-27 Thread Cartun, Richard
I don't have an answer for you, but I have observed this with IHC performed on human tissue where Staph is present (primarily skin biopsies). I have seen labeling of Staph organisms with many different types of antibodies (e.g., CD3, CD20, S-100, and more) and I have always attributed it to bin

[Histonet] Slide remounting

2014-08-27 Thread Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)
Does anyone have a procedure that removes coverslips (w/crystallized permount present) ? The slides are 10+ years old. And is there a process to liquify the crystallized permount and refill w/o removing the coverslip? The mounted slides are 3 x 1's Juan l. Bassett HT ASCP Histotechnician Ana

[Histonet] Ocular micrometers: recommendation of suppliers

2014-08-27 Thread Jorge A. Santiago-Blay
Dear Histonetters: I am about to request the purchase of two ocular micrometers, one for each of two compound microscopes. As there are many suppliers available, any recommendations on suppliers of ocular micrometers that offer them at a reasonable cost will be appreciated. Please, feel free to di

[Histonet] RE: Looking for suggestions, ideas

2014-08-27 Thread Tom McNemar
We did this a few years ago for our local coroner's office. They send us their tissues and we process them and cut the slides. We return the slides and blocks to them so there is nothing in our files. Are you returning the blocks with the slides? Pretty simple to do. I created a new specime