[Histonet] double staining alkaline phosphatase

Dear Histonetters, Is it technically possible to perform a double staining using alkaline phosphatase in both stainings ? I was thinking to perform a seqeuntial staining and develop the staining for the first protein and detect with a blue AP substrate and then perform staining for the second

RE: [Histonet] Flames at embedding centers

My very first Histo job was with Metpath in Hackensack NJ. We had Bunsen burners right next to a xylene bath for dirty molds. One day a tech accidentally had a xylene Bunsen burner collision and started a fire. We had another tech who was quick with head and feet who was able to extinguish it -

RE: [Histonet] Flames at embedding centers

We have days when one of the histologist who has been here for years and I (going in 50years) start talking about the good days and everyone here is under 40. They look shocked and then disbelief and then they think we are kidding. They are so sure we have always known which chemicals were dan

RE: [Histonet] Flames at embedding centers

We're well preserved! -Original Message- From: Horn, Hazel V [mailto:hor...@archildrens.org] Sent: Tuesday, November 18, 2014 4:23 PM To: 'Paula Pierce'; Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flames at embedding centers

RE: [Histonet] Flames at embedding centers

Yes, the good old days!And, we survived! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children’s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original

Re: [Histonet] Flames at embedding centers

The open flames were great because they incinerated any residual tissue fragments, like from EMCs. Hardy ever had cross contamination back in those days. Lab fires, yes - I remember one in the late 1960's at St. Elizabeth Hospital in Chicago. (Just heard about it, wasn't there at the time)

Re: [Histonet] Flames at embedding centers

Like! & ditto! Paula Pierce,BS,HTL(ASCP)HT President Excalibur Pathology, Inc.  5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: "Blazek, Linda" To: Jay Lundgren ; Ludlow Patricia Cc: "histonet@lists.utsouthwestern.edu" Sent: Tues

RE: [Histonet] Flames at embedding centers

Grinning and remembering the "good old days". What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tue

Re: [Histonet] Flames at embedding centers

Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forcep

[Histonet] Flames at embedding centers

Is anyone out there still using flames at the embedding centers for their forceps? If not, what are you using instead? Pat Ludlow Technical Specialist/Supervisor Histology HRLMP 905-522-1155 X35954 This information is directed in confidence solely to the person named above and may not otherw

[Histonet] Microtome ergonimic evaluation

Has anyone ever had an ergonomic evaluation specific to their microtome station? If so, could you please give me the contact information of the evaluator? Thanks, Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory

[Histonet] tissue tek 5 embedding center

Can anyone tell me if the Tissue Tek 5 has a work light? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org

[Histonet] RE: Rubber "mats" for pinning specimens

We use Styrofoam and break it to the right size. Get them out of shipping boxes. Free! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archil

[Histonet] RE: Rubber "mats" for pinning specimens

We make up paraffin trays, either in a cafeteria tray (sssh) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin. Berni

[Histonet] RE: Rubber "mats" for pinning specimens

Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthweste

[Histonet] Rubber "mats" for pinning specimens

Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something

RE: [Histonet] competency checklist

We actually have two. We have the simplified one CAP wants and the comprehensive one we need for our histologists. They are very different. CAP just wants to know basics in a short form as far as our administration was concerned. We developed our own internal to measure how well people are d