[Histonet] RE: Renewing Histonet subscription

I have received notice so I would guess it is a phishing scheme. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Monday, January 19, 2015 2:24 PM To: 'histonet@lists.utsouthwestern.edu'

Re: [Histonet] Recycling alcohol

I used 200 proof ethanol and tried also to recycle it. After making a costs analysis I concluded it was not worth it. Too much waste, too much electricity, too much water and only about 50-60% recovery, at beast.If you have a good ethanol source I would advise not to embark in recycling it. Xyle

Re: [Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC)

Michael, Not sure from your explanation if your mCherry tag is less intense on the inside of sections or if you are referring to the P and RFP Alexa and Cy5 stain intensity  If the former, not sure about that.  If the later, having done very similar thing in grad school, but with different

Re: [Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC)

Is there a reason you probe with the two primaries independently? I stain rat spinal cord and brain, 35 microns thick free floating, with both primary's at the same time. I stain at room temp on a shaker overnight. This seems to work pretty good. Thanks Leslie Sent from my iPhone > On Jan

[Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC)

Hi All, I'm new to graduate school and the world of immunohistochemistry. I am looking for a way to change my protocols so that the tissue sections are stained/penetrated by the antibodies more effectively. I am doing an anti-substance P stain, followed by an anti-RFP stain in 50um sections

[Histonet] Renewing Histonet subscription

Regarding the message Alice sent below that went out to the list.. I have been the Histonet administrator since its inception and I have NEVER heard of a need to renew a subscription. I don't think the server is programmed to ask for that (at least I haven't been notified of such a thing) so

[Histonet] RE: Recycling alcohol

With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240

[Histonet] Recycling alcohol

We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol f

[Histonet] cutting a honeybee

It looks like I will be helping a student get started looking for the invertebrate iridovirus in honey bees that might be involved in colony collapse syndrome. Until we get up to speed, and set up a histolab in my basement, is there someone who will be willing to embed and cut a couple of normal h

Re: [Histonet] HSV I & II control slides

We have HSV I and HSV II control slides in BioPathx. All our control slides are validated. Please visit our website at Biopathx.com or contact us at i...@biopathx.com for more information. Best Regards Biopathx.com > On January 16, 2015 at 1:04 PM "Adesupo, Adesuyi (Banjo)" > wrote: > > > Hel

[Histonet] rodent eye

Good afternoon, I am currently working with Lewis rats performing corneal alkali injuries at varying strengths. Is there someone there that has prior experience working with a rat eye and would be willing to share information on the most effective ways to preserve, fix and cut the cornea sample.