[Histonet] RE: Paraffin-Plastic Stratification/Congo red problem

I believe amyloid does fade with age on cut slides. Frieda sited Bancroft and Cook that intensity decreases with age and "long-standing" deposits stain less intensely than smaller "Newly formed" deposits. She also lists a Crystal Violet method in the 3rd edition, but that it is more of a rapid s

RE: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab

Jim Its based upon the equipment requirements from each operating manual, we use the most critical instrument in the room and what the temp and humidity requirements are for that piece of equipment, we find those are usually the most stringent and the requirements for the other equipment in the

Re: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab

We base our ranges on the equipment specifications. That would be the requirement for the rooms. 30 - 80% humidity for most equipment and the room temp (see equipment manuals) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@met

Re: [Histonet] Paraffin-Plastic Stratification/Congo red problem

I also noticed stain fading with time - controls cut way ahead of staining? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/23/15, 1:00 PM, "Wheelock, Timothy R." wrote: >Hi Jeffery: > >Yes, I notice th

Re: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab

I forgot - reagent temps too! Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/23/15, 1:01 PM, "Vickroy, James" wrote: > >We have always measured the temperature and humidity of the histology >lab. Som

RE: [Histonet] HTL exam

Agree with your points and book recommendations. I do remember some slide ID on mine. Also there are more taxonomy II & III and operations on the HTL, which warrants a slightly different study approach, but completely acheivable. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jrob

[Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab

We have always measured the temperature and humidity of the histology lab. Someone today asked what the normal ranges for a histology lab were? Any ideas? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office:

[Histonet] Paraffin-Plastic Stratification/Congo red problem

Hi Jeffery: Yes, I notice the same "paraffin dust bunnies", perhaps especially since I use a embedding paraffin that has a fair amount of plastic. Before I embed my brain tissue, I mix the embedding media thoroughly, until the solution is clear. As for your Congo Red problem, I wish that I coul

[Histonet] RE: Histonet Digest, Vol 134, Issue 28

COLD plate is way to cold. Regards -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134

RE: [Histonet] HTL exam

I used the NSH booklets on the various Histology subjects. I don't know about their current availability- I think they were on a CD now but I haven't checked lately. I learned a lot from the booklets as they not only give the correct answer they also described why the other answers were wrong

[Histonet] RE: Problem with cracked paraffin blocks

Regarding the paraffin/plastic stratification: we used to get the reddish "paraffin dust bunnies" in the paraffin pots apparently from the separation and stratification of the paraffin and the plasticizers. I bought a big wood paddle from the industrial kitchen supply store (about $12) and we

[Histonet] HTL exam

Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the HT with just reading an older Frieda Carson book. Can someone give me some advice on books that really helped them? Thanks! Sent from my iPhone > On Jan 23, 2015, at 1:01 PM, histonet-requ...@lists.utsouthwestern.ed

RE: [Histonet] Amyloid by Congo Red

We do all of our Congo Red staining on Muscle and Nerve frozen sections. Sometimes when there is very little amyloid detected using the bright-field/polarizing microscope our pathologist will confirm under a fluorescent microscope with the Rhodamine Red filter. This shows the amyloid inclusio

[Histonet] HT Program Application

Hello Everyone, Florida State College at Jacksonville is now accepting applications for the 2015-2016 class of Histologic Technology. This is an online program with scheduled laboratory session at our north campus. For more information, please visit our website at http://www.fscj.edu/academic

Re: [Histonet] Amyloid by Congo Red

What protocol and reagents are you using for the stain? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/23/15, 10:43 AM, "Jeffrey Robinson" wrote: >Greetings to all Histotechs- Here's an amyloid ques

[Histonet] Problem with cracked paraffin blocks

Hi again everyone: I want to thank you all for your advice. The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. I will run some test blocks over the weekend, and then embed th

[Histonet] Thermo IHC

Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can anyone help us cut through the *88*888 and give me some helpful tips? We have practical experience on the Ventana systems only. Has anyone been successful at using only 1 HIER buffer? Any tips on how to shorten the

[Histonet] Amyloid by Congo Red

Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive fo

Re: [Histonet] Cracking paraffin blocks

Hello Tim, I also have had this cracking problem in the past but since resolved. I'm not sure about the specifics of the cracking problem but the cold plate might have something to do with it. Our cold plate is set to -5C and use Paraplast from McCormick Scientific. Our paraffin is roughly the s

RE: [Histonet] Cheap Disposable Blades for Facing In

We are doing the same. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] Cracking paraffin blocks

Hi Everyone: Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. I am having problems now with blocks developing cracks on the cold plate. The cracks run either through the wax right next to the specimen, or more frequently, r

RE: [Histonet] rodent eye

Hi Casie, Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet

[Histonet] hpv

We are having problems finding someone to do HPV staining. Does anyone do HPV staining? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CCK testing

Dear all, If there is anyone that is doing crystal identification in bile fluid could you contact me off list? Thanks, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com

Re: [Histonet] Cheap Disposable Blades for Facing In

We save our blade from the previous day to use for facing. We keep them in slide mailers. Sent from my iPad > On Jan 22, 2015, at 4:21 PM, Sandra Cheasty wrote: > > Hello everyone, > > Does anyone have a source for cheap, low-profile blades for facing in blocks? > > Thanks! > Sa