Dear ynw...@u.washington.edu.
Boiling makes steam, with bubbles that greatly change tissue structure. Slow
freezing is just as bad; the ice crystals make holes that deform and replace
the tissue architecture. What are you tryng to find out? It has been known for
100+ years that boiling collagen
Hi Bonnie, we use some from Brain Research laboratories. They are cardboard
flats without the dividers that are in the 20-slide types .
They have 2x3 slide mailers as well.
http://brainresearchlab.com/product-category/slide-storage/slide-folders/
Tim Morken
Supervisor, Histology, Electron Mic
Short of using a bar-coded tracking system, does anyone use "color-coded"
formalin containers or cassettes for prostate biopsies (for the urology office
staff) to confirm the patient's identity? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospec
HI Renee- I had a major problem in the past with tissue staining for IHC
lifting off the slides no matter what I tried. It turned out that when we
precut our controls and put them in the oven and then used them later for the
patient tissue that the charge on the slide was altered. We currentl
Hi Renee,
Hope you don't mind but I have had a lot of experience in this area. I worked
for Ventana for 7 1/2 years as technical specialist and now as a Technical
Support Manager and I know that getting sections to stick to slides can be a
bit challenging especially during antigen retrieval.
Hi All,
What slide folders do any of you recommend for use with 2x3" slides (we use
them for whole-mount prostates)? If you have some that work well, could you
send me the ordering info?
Thanks,
Bonnie
Bonnie Whitaker
AP Operations Director
The Ohio State University
Wexner Medical Center
Dep
Utah is in NSH region 7 and your regional director is Jane Parr,
jane.p...@ucdenver.edu . I would contact her to double check.
Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wine...@novusint.com
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From: histonet-boun...@
Help, sections not sticking to charged slides. We use Mercedes Medical charged
slides. Lately have been using sta-on but still have occasional problems
especially during antigen retrieval. I need any suggestions.
Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
R
?Hello Histo folks. Are there any Utah Histo Tech subscribing or anyone with
the information that Utah has a Histo society? Would really like info.
Thanks in advance
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*Is your lab in need of a new microscope? We have several Olympus BX40 and
BX41 compound microscopes in stock! and offer a 1 year warranty on all
scopes. *
*All microscopes are fully refurbished and are priced at 40% off list. The
scopes are serviced by an Authorized Olympus Service Tech before
Thank you Dr. Kiernan, Nice and scientific explanation how to prepare gelatin.
I beleive that a lot of house wives and cooks know how to prepare meet or fish
jelly (long bouling of the bones with cartilage and tendons). very popular in
russian or easter europian cuisine, called "cholodez" which
Hello histonet,
Has anyone out there dealt with a busted Heat Resistor in a CBG reagent
recycler.
Any thoughts or insights appreciated.
Thanks in advance,
Ryan Roy HTL (ASCP)
Manchester Veterans Affairs Medical Center
Manchester New Hampshire
Disclosure: The content of this email does not re
If you are using an automated stainer the plexiglas cover that they all have
will block UV light. For instance we run all our IF in a Dako stainer with the
slightly tinted Plexiglas and have not had any problems. If staining manually
in a tray, covering with something to block light is ok. On
Hi Allison,
I would suggest going to the CDC website and pulling from there, they should
have the latest recommendations. thanks
Debbie Siena
800.442.3573 ext. 229 | www.statlab.com
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From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsou
Hello to all in histoland. Does anyone have a procedure for handling
creutzfeldt-jakob disease. Any help will be appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, pleas
As long as your microscope has a good Hg source light, it is not really
necessary to darken the room so much. I used to have a very small room were the
microscope was located but later on we moved the microscope to another area
were we did not dim the light at all and the vision was OK.Everythin
Thank you for your e-mail.
Apologies for not explaining "treated tissue". We treat the tissue with
high intensity focused ultrasound. It can raise the temperature of tissue
to boiling in a localized area (millimeter areas). I could use a
biochemical assay for collagen and gelatin if we treat a lar
Good Morning Netters,
While running immunofluorescence stains, how dark is dark enough?
I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.
I feel that dimmed lights are good enough.
What does the hi
That's interesting, I didn't realize gelatin is the water soluable protein of
collagen. No experience with staining gelatin, but have you considered
MassonTrichrome.
It is used to differentiate collagen from smooth muscle...
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