[Histonet] RE: Ventana Ultra CD10

For Ventana CD10, (SP67), we use the extended CC1 (92 minutes), antibody for 36 minutes and amplify on the Ultra. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- Fr

[Histonet] RE: cilia for EM

Our clinicians use a brush of some sort. Unfortunately I don't know the brand. But the bristles do not come off; they are pretty stout, are in a spiral pattern and each bristle has a knob at the end. We just gently scrape the cells off the brush into fixative in a petri dish and then pipet up an

[Histonet] Ventana Ultra CD10

We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras. During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks. My Venta

[Histonet] RE: cilia for EM

My colleagues tell me a curette is the best instrument to use to collect ciliated cells from the nasal turbinates for electron microscopy. Presumably this would avoid the problem of bristles coming loose. I think this is the device described in this publication (please correct me if I'm wrong

Re: [Histonet] RE: Expert opinion vs. second opinion

I consider both as identical because you are not going to seek a "second opinion" from somebody is not an expert.Asking another person with not real expertise for an opinion is nonsensical.René J. On Thursday, February 26, 2015 12:41 PM, Sharon Scalise wrote: I am looking for clari

[Histonet] RE: cilia for EM

Will respondents please be sure to click "reply all" to this post? We're having the same issue here. Namely, remnants of the sample collection brush remain in the vial and damage our diamond knives. Any advice would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology

[Histonet] cilia for EM

Does anyone have a good technique/tool for cilia biopsy collection that we could share with our physicians. We have been having issues with poor quality specimens. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue

[Histonet] RE: Expert opinion vs. second opinion

I am looking for clarification about billing for expert opinion vs. second opinion. Can someone explain when and how each is used and billed so that I can pass this along to upper management. Thank you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

Re: [Histonet] CD68 and CD204 for dogs/pigs

Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue. The PI is > asking for additional CD markers to macrophages (CD68 and 204). I need > t

Re: [Histonet] CD68 and CD204 for dogs/pigs

Hi all, I do have MAC387 and it works great in dog and pig tissue. The PI is asking for additional CD markers to macrophages (CD68 and 204). I need them to work on dogs and pigs, but of course, there's not many vendors who list species cross-reactivities on their data sheets beyond the usual hum

[Histonet] Oil Red O

Hello Fellow Histonetters, We often get slides from another hospital for Oil Red O staining. On occasion, they send them at room temp. and until they get to us it's been a day or more. I'm worried that because they are unfixed the cells will be autolyzed and I don't want to charge the patient