Re: [Histonet] Crystallized formalin

2015-05-26 Thread John Kiernan
The formaldehyde in formalin is present as low polymers. The depolymerize when the liquid is diluted. With storage, especially at lower temperatures, the small polymers join together anf form large insoluble polymers, known as paraformaldehyde. This reduces the effective concentration of formald

[Histonet] Who are you guys using to service equipment in the San Diego area?

2015-05-26 Thread Bea DeBrosse-Serra
Who are you guys using for servicing different equipment like microtome, cryostat, stainers in the San Diego area? Thank you in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 ___

[Histonet] NSH Awards

2015-05-26 Thread Elizabeth Chlipala
Hello Everyone It's getting really close to June 1st which is the deadline for nominations for NSH Awards. I know that you have heard me say over and over again how important it is to nominate yourself of other individuals for awards but I am going to keep doing it. So here it goes... Th

Re: [Histonet] Waterbath cleanliness

2015-05-26 Thread Sanders, Jeanine (CDC/OID/NCEZID)
Hi, Paraffin build-up should not be a common practice. I recall being told as a student decades ago, "The waterbath should be kept scrupulously clean". Some things are still true today and this is one of them. Does your waterbath have a glass insert? If so, simply wipe off all of the paraffin

[Histonet] Crystallized formalin

2015-05-26 Thread Julio Benavides
Hi there, we have a bottle of formaline 40% with crystals at the bottom. Can we used it? is there any way to re-suspend the crystals? Better dispose of it? and, BTW, do you know why it crystallized? by its side there is another bottle, same batch, with no crystals. thanks a lot your comments

Re: [Histonet] dehydration time for a relatively large sample

2015-05-26 Thread Rui TAHARA
Thank you for your advice! I was going to try if the current sample that was embedded by a technician at the histological service center. This is the first time that i asked for an expert to prepare my sample. Then I sectioned the sample by myself. However, the muscles in the section always look