Thanks to all who responded to my question regarding PAS staining!
We got much better staining after following you guys suggestion:
a. we purchased reagent from American Mastertech
b. we used 1% periodic acid
c. we rinse slides under warm water after Schiff's step.
Thanks again,
Amy
A position is open for a lead technologist at UCSF Medical Center, San Francisco
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Search "Histotechnologist"
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Tim Morken
Pathology Site Manager, Parnassus
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Cover it loosely, and warm it up a little with a stir bar, under a hood.
You should be able to tell the difference between alcoholic and aqueous
formalin by smell. Why isn't it labelled?
Sincerely,
RE:
we have a bottle of formaline 40% with crystals at the bottom. Can we used it?
is there any way to re-suspend the crystals? Better dispose of it? and, BTW, do
you know why it crystallized? by its side there is another bottle, same batch,
with no crystals.
>
> thanks a lot your comments
>
> R
