Re: [Histonet] Labeling formats

Thanks to all for your feedback. If anyone else has different formats, please let me know. Also….is anyone out there printing patient NAMES on the blocks? Just curious… THanks Dr. Ruby On Oct 2, 2015, at 3:09 PM, Kim Donadio mailto:kdona...@ymail.com>> wrote: Dr Ruby, All places I've worked a

Re: [Histonet] Missing specimen

I've had something like this occur before. Unfortunately things did not end well. The prostate needle biopsies were labeled for two separate patients were labeled the same accession number and patients name. After playing the guessing game and sent those results out without a DNA they had remove

Re: [Histonet] Labeling formats

Dr Ruby, All places I've worked at use similar as well. Also you need a second patient identifier as blocks and slides are considered an aliquot from the original specimen. Such as last name, date of birth etc And it doesn't have to be S, it can be any prefix meaningful to your

Re: [Histonet] Missing specimen

Stand your ground. You're right! Patients lives are not guessing games. I wish you the best and must forewarn you. Choosing integrity in this day and age is a lonely road and often doesn't pay well. But your conscious will be clear and it's the right thing to do. ___

[Histonet] Acid Phosphatase, Leukocyte (TRAP) Kit (387A SIGMA)

I was having issues using this TRAP Staining Kit for osteoclast activity. I followed the protocol, but did not counterstain. I checked the slides and saw no reactivity. I did notice golden cluster of cells within the bone marrow, but nothing red/purple. I am thinking that the (EDTA) decal proces

Re: [Histonet] Missing specimen

You can not speculate. You have no chose but to wait on the DNA results. This could become a legal case at some point so there is good reason to not speculate but have indisputable proof Sent from my iPhone > On Oct 2, 2015, at 10:47 AM, Jennifer Clark wrote: > > > I have a situation that has oc

Re: [Histonet] Labeling formats

Additionally, we are all moving to a digital world of accessioning and labeling. The days of leaving out the "A" or "1" to indicate that there is only one part or one cassette need to become a process of the past. LIS as sighed labeling can not accommodate this format. Sent from my iPhone > On Oc

Re: [Histonet] Labeling formats

This is a format that I would recommend. To expand on that, using an "alpha" character for the part and " numeric for the cassettes makes better sense because there are few cases that have more than 26 parts (causing AA, AB, AC labeling rare), but there are many parts that have more than 26 casset

[Histonet] Tempature Logging

Karen, We were hit with minor incident by CLIA for not having Temp and Humidity documentation as well. We also use the equipment manual to set the Temp and Humidity operation ranges. WE have a hi/ lo Temp and Humidity monitor on the door jam between both rooms. We hand document the time and the

Re: [Histonet] Labeling formats

Ours is very similar...only difference being ours is: 15S-1234A-1 (year-prefix-accession number-part(alpha)-block number(numeric). Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (

[Histonet] Missing specimen

I have a situation that has occurred and am unsure how to proceed. One MA called to get results for a young patient. We had no record of a specimen for this patient at all. The doctor was sure it was performed. With some research, we discovered that another patient, middle aged,(who was in

[Histonet] Labeling formats

I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device

Re: [Histonet] autoradiography frozen human brain sections fall off, white matter issue

Hi Esther, We do a lot of autoradiography on rodent, primate and human brains sections. Our protocol is similar to yours except we do not dry the slides after incubating in the buffer and before adding the radiotracer. The experiment is performed with the slides in a rack and totally immersed i

[Histonet] GMS and Gram stains

Hi All Netters, I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic. Thanks, Mehndi Helgren Dominion Pathology Labs Norfolk, VA (AKA Hurricane Central) :) ___ Histo

[Histonet] autoradiography frozen human brain sections fall off, white matter issue

Dear Histonetters, Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ? We have problems with falling off sections of human brain tissue. We have done the same experiments and cutting in rodent tissue without any problems

[Histonet] Opinions about the Intavis InsituPro system

Dear All, my department is considering the purchase of an Intavis InsituPro VSi system (http://intavis.com/products/automated-ish-and-ihc/insituprovsi), and I'd be very interested in any feedback I could get on this instrument. Our interests are in plant biology, for both immunofluorescence and i