Hi Monica,
The majority of fluorescent proteins (eGFP, RFP, Cherry, Tomato) are well
preserved after 4-6h fixation with 4% formaldehyde in PBS at +4 C, following
PBS washing, sucrose cryo-protection and embedding in OCT. Bright fluorescence
is easily seen on cryo-sections.
Alternatively, card
Happy weekend to all
http://www.chicagonow.com/downsize-maybe/2016/03/march-madness-bracketology-presidential-style/
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
The Region 1 Histology Meeting sponsored by the Connecticut Society for
Histotechnologists, Inc. is being held April 15th and 16th at the Mystic Hilton
in Mystic, Connecticut. We have three lecture rooms going both days for a total
of 24 speakers, plus a full vendor hall.
Local attractions incl
For those of you who do FISH...
http://www.chicagonow.com/downsize-maybe/2016/03/fish-a-little-known-lab-test-for-an-important-disease/
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
Decolorize Congo Red by soaking in a 1% alcoholic solution of Sodium
Hydroxide. Heat and stirring might help, be careful not to soak your
sections off the slides, NaOH is a strong base.
Jay A. Lundgren, M.S., HTL (ASCP)
On Thu, Mar 17, 2016 at 1:13 PM, Nancy Schmitt
wrote:
> Hi
Does anyone have a knife holder for an old JB4 microtome that they would be
willing to part with, for a price of course?
I was given an old JB4, I have the base and the specimen holder, but need the
glass knife clamp.
Thanks,
Sharon
sbled...@iupui.edu
This email has been scanned by BullGuar
Are you sure you don't mean Oil Red O stain for fat? Just checking.
Congo Red is for amyloid.
Destain Congo Red by soaking in 100% Propylene Glycol, warmth and stirring
would speed it up.
Jay A. Lundgren, M.S., HTL (ASCP)
On Thu, Mar 17, 2016 at 12:41 PM, Nancy S
Karen,
I agree with Lester, you need support. Unfortunately, histology often
gets "lumped in" with clinical laboratory medicine (in my experience) and our
leadership is often a med tech. I have nothing but sincere respect for medical
techs, anatomical pathology is just a different animal
Hello, I'm looking for a source for acquiring or purchasing good positive
controls (slides or blocks) for the following antibodies:
- Pan Cytokeratin (AE1/AE3)
- Cytokeratin 5/6 (CK 5/6/)
- HPV Cocktail Broad Spectrum (1, 6, 11, 16, 18 & 31)
- HMW CK + p
