Hi.
Back when I was in research we did Hale's Colloidal Iron stain. Making the
dialyzed iron was really the big part and back then we were using a
cellulose tubing that we had from another group. I've been trying to find
the right type but there is just too many choices. I've also been trying
t
We add 50mls of 1% eosin to our first alcohol after the formalin.
1% EOSIN
FOR PROCESSING BIOPSIES
100mL ACETIC ACID
2g EOSIN
100mL DISTILLED WATER
Stir well!
Lisa Ryan
Histology
St James hospital
Dublin Ireland
From: histonet-requ...@lists.utsouth
Hello Rebecca,
Reguarding the following message:
"I had a biopsy today that was nearly impossible to see on the sponges during
embedding or in the block. I've heard mention of marking these with eosin to
make them easier to see. Has anyone done this? Or do you use some other type
of markin
We do the simple, quick crystal violet for amyloid and one of the Drs. would
like for it to be lighter. We make stock soln from powder, and then fresh
working soln each time. Using a procedure from the 1990 Carson (pg 134-135).
Basically the slides get dipped and then rinsed. There's no diff
Rebecca Ashley at the Wyoming State Vet Lab in Laramie asks >> I had a
biopsy today that was nearly impossible to see on the sponges during
embedding or in the block. I've heard mention of marking these with eosin
to make them easier to see. Has anyone done this? Or do you use some other
type of ma
We use safranin at the grossing station and it is a dark pink at embedding.
Works really well in our hands. Added plus is no fluorescent issues that you
can have with eosin.
Cindi
From: Cindy Bird via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Th
