Re: [Histonet] block scrapers

Use a regular one blade pocket knife (as I used to do).René On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet wrote: Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me?

Re: [Histonet] Reticulin Stain

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[Histonet] H&E staining on tape

Hi everyone, I have a colleague that has been asked to do H&E staining on a frozen specimen collected on tape. The tape has been attached to the slide using nail polish. Can't use the CryoJane system due to the light flash. Her question is "will the nail polish disintegrate during the immersion

Re: [Histonet] block scrapers

Yes, search paratrimmer you will love it. It melts excess paraffin off blocks. JB On Thu, Jul 21, 2016, 11:52 AM Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of the > blocks after embeddi

Re: [Histonet] block scrapers

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Re: [Histonet] Reticulin Stain

Hi All Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral Red

Re: [Histonet] block scrapers

I bought everyone really old pocket knives from flea markets--basically the same thing as what Jay said. For the most part, these old knives were already really dull and didn't require any assistance to dull them further. Good luck. Brian -Original Message- From: Jay Lundgren via His

Re: [Histonet] block scrapers

Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of the > blocks after embedding. Does anyone

Re: [Histonet] block scrapers

Lauren I have always used a very dull steak knife (sharp ones are too sharp and will cut the cassette) mine is probably over 20 years old (now I'm dating myself). I'm old school so I still use that but the rest of the techs will use the para trimmer. The para trimmer is more ergonomically fri

[Histonet] block scrapers

Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.

[Histonet] Unscribe

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Re: [Histonet] MCH IHC

Hi Estela- I had the same problem with the Bond multicytokeratin. I currently have 3 vials of the "old" one on backorder. I had to validate the new "reformulation" as I have now run out of the old one (PA0909). Have your rep send you the new one (PA0012). My rep sent me one for free. It se

Re: [Histonet] Reticulin Stain

Gomori's René On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet wrote: Does anyone out there still do the retic by hand?  If so, can you share which procedure you use?  Thanks ___ The information contained

[Histonet] MCH IHC

Hello My Histo Friends!! Does anybody have an extra MCK RTU Antibody-- Catalog No. PA0909-- Clone AE1 and AE3, for the Leica Bond Max machine. that we can borrow until we get ours in. Apparently it's been back ordered til Aug 1 and we are out. Please let me know if someone can help us out. T

[Histonet] Best semi-automated/automated TMA instrument to buy?

Dear all amazing histology people, Our lab is looking to buy a New great semi-automated/automated TMA instrument. I worked before on manual Beecher and semi automated Veridiam. They are just ok. Do you have any suggestions? I think Beecher has an automatic one? Thank you and have a wonderfu

[Histonet] Reticulin Stain

Does anyone out there still do the retic by hand? If so, can you share which procedure you use? Thanks ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for

Re: [Histonet] Fading of H&E controls -long response with portions of SOP

James We use recycled xylene in our H&E stainer and have never experienced any fading of the eosin. When you recycle your clearing agent do you check for purity and contamination, you have to also take into consideration that xylene substitutes may behave differently than xylene. We have an

Re: [Histonet] [External] Crystals in PAS from Ventana

Hi Mark, We still have this issue after two years of complaining to Roche. We've had multiple TAS's come in and we've sent slides to Tucson. They've gone over the instruments, etc. Long story short, their fix was adding something to their package insert that says "Schiff's Reagent is known to

[Histonet] Fading of H&E controls

We are getting ready for our first CAP inspection in the new laboratory.I was checking over the H&E controls that we do daily and made a discovery. Several of the first slides we stained, over a year ago, are now washed out. The eosin staining is probably worse than the hematoxylin howeve