Re: [Histonet] PFA Fixed Tissue not sticking to slides?

Thank you Charles Scouton for responding. The slides are positive charged slides from IMEB. Thank you Elizabeth Chilpala for responding. The treatment after sectioning is one I’ve forwarded to the service that is processing for us. I only do the PFA/sucrose/embedding part. My understanding

Re: [Histonet] Question about prelim report for autopsy with neuropathology

Hi Tim, In the hospitals I have worked, we used the PAD simply to communicate gross pathologic diagnoses, so we would issue a gross description of the brain (like the brain weight compared to normal, edema, atrophy, herniation, hemorrhage, etc), "pending microscopic examination", and then follo

[Histonet] Question about prelim report for autopsy with neuropathology

How do you handle counting days to preliminary autopsy dx (PAD) when doing cases in which the brain autopsy results are the main component of disease? If we accession at the time of autopsy and then let the brain fix for several days before cutting in, then the PAD is many days past the 3-day re

[Histonet] B-5 Fixative

Hello! I am wondering how many labs are using B-5 fixatives and how many are using B-5 alternatives due to the mercury issue? For those who have switched to an alternative, have you noticed many differences from the traditional B-5 fixative? Any and all feedback would be greatly appreciated! :

Re: [Histonet] PFA Fixed tissue not sticking to slides?

Are you using a good plus slide? We have started using these Tru-Bond slides when we have tissue adherence problems, they seem to work really well. How are you handling the slides once you section? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 8

[Histonet] PFA Fixed tissue not sticking to slides?

Dear HistoFolks, Has anyone had experience with cryo-sectioned tissue that is PFA fixed not adhering to slides? The tissue is rabbit cornea culture, 4% PFA fixed O/N, then run through 20% sucrose gradient, then frozen in OCT. We think that the PFA fix is much longer than needed for a tissue th

[Histonet] Automated Rotary Microtome

Hi, We are in the process of purchasing Automated Rotary Microtomes and I will appreciate recommendations from you guys. Have a great weekend. Sincerely, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307-

Re: [Histonet] Distilled Water vs DI water for IHC and H&E staining

Caroline, As a vendor I have had the privilege to visit several hundred labs throughout the United States and in other countries. From what I have seen, I would say the the answer to your question is it depends on the quality of your local water source. In most cases city water systems a rather

Re: [Histonet] Feedback on Immunohistochemistry stainers

Request a DAKO demo.René On Thursday, July 28, 2016 8:01 PM, Gmail via Histonet wrote: Hi all, We are looking into getting a new staining platform for our IHC lab. I would appreciate any feedback from your experience regarding ease of use, how long maintenance;daily , monthly takes t

[Histonet] Christmas in July?

Hi Histonetters! How are you? I hope you have a great weekend! It's Christmas in July! First of all THANK YOU for taking the time to read my post. Every couple of weeks I post a career bulletin listing all of my current openings and I appreciate the space you allow me on the forum and in your