Re: [Histonet] ATM IHC

I have done this in the research world, but I don't have any experience with clinical analysis of this protein. I will tell you that cell signalling have the best antibodies in this space, IMHO: https://www.cellsignal.com/browse/?Ntk=Products&N=4294956287&Ntt=atm+ A company that you can actually

[Histonet] ATM IHC

Anyone doing IHC testing for "Ataxia-telangiectasia-mutated protein" expression on FFPE tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant D

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background c

Re: [Histonet] Antibodies storage tips

We use freezer boxes and then record what is in the freezer boxes on a worksheet that is kept in an excel file. The excel file has all of the boxes in it. Here is an example of one of the pages BOX 6 - Full name vendor catalog # lot # date alqt ul/aliquot notes AGE Biorbyt

Re: [Histonet] Caspase 3 and Tunel Assay

Ronnie We have these protocols in place for porcine tissue. We use the roche kit for tunel 11684809910 and for CC3 we use the cell signaling antibody 9664. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060

[Histonet] Caspase 3 and Tunel Assay

Is anyone working on this just now? And more specifically on the pig species? Any pointers would be appreciated Thanks Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nati

Re: [Histonet] oil red in WAT frozen samples

Apply gently heated water on the sections in a way that the gelatin is washed out.René On Tuesday, August 16, 2016 4:48 AM, Monica Aguilera via Histonet wrote: Dears, I was wondering if some of you might have experience in the following: We have had a lot of problems cutting WAT tiss

Re: [Histonet] Antibodies storage tips

I used to have several compartmented plastic alphabetized boxes with enough empty spaces to accommodate "new arrivals". If the spaces were used-up I just added a new box. Since they occupied several shelves, each shelf had the lettering identifying the boxes in each one.René On Tuesday, Au

Re: [Histonet] Placing formalin on specimens

Usually you do not pour 10%NBF onto a specimen; you place the specimen onto a container/vial with the 10%NBF.René On Tuesday, August 16, 2016 9:29 AM, Mike Pence via Histonet wrote: I know this might sound a bit crazy, but does anyone have a written policy and procedure for dispensing

[Histonet] Placing formalin on specimens

I know this might sound a bit crazy, but does anyone have a written policy and procedure for dispensing 10% formalin onto a surgical specimen? If you do, would you be willing to share? Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlin

[Histonet] Antibodies storage tips

Hi all, In our lab we carry out quite a number of IHCs for research purposes. The number of mAbs and pAb stored in the frezeer is getting to the point that, when you need to do and IHC, you actually lose more time diving through the frezeer draws for the right vial than doing the IHC! is not

[Histonet] oil red in WAT frozen samples

Dears, I was wondering if some of you might have experience in the following: We have had a lot of problems cutting WAT tissue either directly embedded in OCT or pre-fixed with PFA or formalin (either cold or and them embedded with OCT. We solved this problem following Ryan Berry et al, 2014 pape