It's endogenous biotin, Tyrone.
Make your own biotin blocking kit or buy RTU kit.
Also, endog. peroxidases are eliminated by 3% aq H2O2 ( saves methanol) for
10mins at RT
Also, don't bother with permeabilisation.you've done all that by processing
the tissue to Pwax
Also, use 10% Formalin.
Get a good night's sleep and eat a good, high protein breakfast!
There's always a question about which fixative to use or not use with uric
acid crystals/gout, so I just gave you a free one!
Good Luck!
Jay A. Lundgren, M.S., HTL (ASCP)
On Wed, Sep 14, 2016 at 7:56 AM, Van
Back in the 1960's when I was learning all about the PAS method, I got my hands
on a new CRYO-Stat (-30_!!!) and tried my 'stuff' on fresh frozen
sections. Having been in the habit of controlling every variable I could name,
I included a 'Schiff' control with each PAS.
Good morning Mr. Genade
Try using acetone/H202 in your blocking step. It may solve your problem with
endogenous peroxide.
Use the same amounts you would use for your blocking solution. I hope this
helps.
Cynthia James
Sent from Mail for Windows 10
From: Tyrone Genade via Histonet
Hello,
I am doing IHC on fish tissues, in particular the head kidney.
I am using DAB as chromogen. The tissue was fixed in Davidson's Fixative
and processed for wax. Sections were dried in an oven overnight and then
dewaxed and citrate was used for heat antigen retrieval. The sections were
then p
Good morning all!
I am getting ready to take my HT Certification Exam and was wondering if
anyone would be willing to share some advice or thoughts on the exam which
may help.
Thanks in advance!!
Vanessa Keeton
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